Phospholipids and L-Carnitine Screening in Children with Congenital Heart Diseases Undergoing Surgical Correction

Abstract

Objectives: Congenital heart defect (CHD) represents almost 33% of all major congenital deformities, representing a worldwide health problem. The aim of the study is to identify the value of lecithin, cephalin, sphingomyelin & other phospholipids screening in the pathogenesis and prognosis of CHDs and consequently improve their management. Methods: A total of 89 child with CHDs were included [35 atrial septal defect (ASD), 27 ventricular septal defect (VSD) and 27 patent ductus arteriosus (PDA)] and 34 child as a control group. Biochemical analysis of the plasma levels of total and different component of phospholipids for both CHD and control group were done by colorimetry and two-dimensional thin layer chromatography. Assay of plasma L-carnitine level was done by ELISA for both patients and control group. Results: The overall results of the present study revealed a significant reduction in the total phospholipids among CHD patients in comparison to the control group; also, a significant change in the phospholipid profile. A significant lower plasma L-carnitine levels in the CHDs group when compared with the control group (p < 0. 001). Conclusions: Disturbed total and differential types of phospholipids &plasma L-carnitine levels occurs in children with CHDs. Moreover, cell-specific targeting of L-carnitine and phospholipid biosynthetic pathways might serve as a possible strategy for helping favorable outcome in management of CHDs.

Predictors of Spontaneous Closure of Atrial Septal Defect and Ventricular Septal Defect in Children

“…There was a important reduction in lecithin levels in CHD group in contrast to normal kids (p value <0.001). The lecithin/cephalin ratio for the control group was 5.62, for ASD was 2.77 and for VSD was 3.19 [19]. Farouk et al [19] established that the findings of this study show that the phospholipids and the Lcarnitine levels have a valued and significant role in the pathogenesis and prognosis of congenital heart illness with right or left ventricular size or pressure overload as in ASD or VSD.…”

“…The lecithin/cephalin ratio for the control group was 5.62, for ASD was 2.77 and for VSD was 3.19 [19]. Farouk et al [19] established that the findings of this study show that the phospholipids and the Lcarnitine levels have a valued and significant role in the pathogenesis and prognosis of congenital heart illness with right or left ventricular size or pressure overload as in ASD or VSD. Cellspecific targeting of L-carnitine and phospholipid biosynthetic paths could help as a potential strategy for helping in treatment of congenital heart illnesses.…”

“…There was statistically important higher total phospholipid between the patients with natural closure than reduction to ≤ 3 ml than residual >3 ml. This study were maintained by study of Farouk et al [19] as they informed that CHD children had a important low plasma L-carnitine level other than the control group (3.8±1.7pg/ml). Also, there was low plasma L-carnitine level between kids with VSD(1.3±0.7pg/ml) other than those with ASD(1.6±0.5pg/ml) and PDA(1.4±0.6pg/ml), p value < 0.001for all.…”