Phospholipids are synthesized in the G2/M phase of the cell cycle

Lin, Weiyang; Arthur, Gilbert

doi:10.1016/j.biocel.2006.10.011

Cited by 16 publications

(15 citation statements)

References 19 publications

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“…It has been shown that previously internalized plasma membrane is recycled to the cell surface as the cells divide; 13 however, de novo synthesis and degradation of different PL at the end of mitosis has also been documented. 7,[14][15][16] In addition, nuclear envelope reassembly and expansion occurs upon mitotic exit and involves extensive membrane reorganization. 17,18 Fatty acids (FA) are essential building blocks of PL, thus a correct supply is required for membrane synthesis.…”

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De novo fatty acid synthesis at the mitotic exit is required to complete cellular division

et al. 2014

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De novo fatty acid synthesis at the mitotic exit is required to complete cellular division

et al. 2014

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“…The old medium was removed and replaced with 10% FBS-supplemented growth medium containing 100 μg/mL nocodazole (Sigma-Aldrich) and the cells were incubated for approximately 24 h for synchronization to G 2 /M phase. After the observation of round cells, the dishes were gently shaken and the cells at M phase were collected in the old medium (Lin and Arthur 2007).…”

Section: Cultivationmentioning

confidence: 99%

Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy

2009

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The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) revealed that the laser phase shift of cells in the G 2 /M phase was markedly higher than that of cells in the G 0 /G 1 phase. Even in the synchronous cultures to G 0 /G 1 and G 2 /M cell cycle phases, the laser phase shift of the cells in the G 2 /M phase was markedly higher than that of the cells in the G 0 /G 1 phase. The analysis of the cultures of MSCs from different donors with the addition of FGF2 at different concentrations revealed that there was a marked negative correlation between the average phase shift and mean generation time. In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM.

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“…13 This forms the basis for the avidity of tumors for this tracer. In general, rapidly replicating tumor cells appear more avid on 18 FDG PET. 14 Therefore, it is not difficult to grasp the importance of PET imaging on survival in various tumors such as Hodgkin and non-Hodgkin lymphoma, 15 lung and colon cancer.…”

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confidence: 99%

“…prostate cancer). Moreover, given the high glucose uptake of normal brain tissue, imaging cerebral tumors with 18 FDG has proved to be very difficult.…”

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confidence: 99%

“…Therefore, phospholipid biosynthesis is necessary prior to cell division and occurs both during S-phase of the cell cycle (DNA replication) 17 and during the G2M phase. 18 The two most abundant membrane phospholipids are the amino alcohols phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Recent work has shown that tumor cells have an abnormal PE to PC ratio with their membranes enriched in PE.…”

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