Phosphonate production by marine microbes: exploring new sources and potential function

Acker, Marianne; Sl, Hogle; Berube, Paul M.; Stepanauskas, Ramunas; Dj, Repeta

doi:10.1101/2020.11.04.368217

Cited by 9 publications

(15 citation statements)

References 112 publications

(170 reference statements)

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“…Modeled dFe was averaged over 12 months at a 0.5 degree grid in 5 m 3/23 depth bins from the upper 250 meters. Metagenome sequence data and associated metagenomes with environmental variables were quality controlled as described earlier [34,35].…”

Section: Data Sourcesmentioning

confidence: 99%

“…All Prochlorococcus genomes were searched for siderophore biosynthesis potential using antiSMASH v5.0 [42], which searches for known non-ribosomal peptide synthetases and polyketide synthase siderophore biosynthesis pathways. The true proportion of genomes with the siderophore transport cluster was estimated by accounting for genome incompleteness in the single-cell genomes as described earlier [34].…”

Section: Data Sourcesmentioning

confidence: 99%

“…Emmeans [49] v1.6.0 R package was used to estimate the magnitude and statistical significance of marginal means for each covariate in the model. The seasonal effects in time-series metagenomes were estimated using Generalized Additive Mixed Models and Linear Mixed-Effect Models with the mgcv v1.8-26 and nlme v3.1-148 libraries in R as described earlier [34].…”

Section: Data Sourcesmentioning

confidence: 99%

See 2 more Smart Citations

Siderophores as an iron source for Prochlorococcus in deep chlorophyll maximum layers of the oligotrophic ocean

Hogle

Hackl

Bundy

et al. 2021

Preprint

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Prochlorococcus is one of the most abundant photosynthesizing organisms in the oligotrophic oceans. Gene content variation among Prochlorococcus populations in separate ocean basins often mirrors the selective pressures imposed by the region's distinct biogeochemistry. By pairing genomic datasets with trace metal concentrations from across the global ocean, we show that the genomic capacity for siderophore-mediated iron uptake is widespread in low-light adapted Prochlorococcus populations from iron-depleted regions of the oligotrophic Pacific and S. Atlantic oceans: Prochlorococcus siderophore consumers were absent in the N. Atlantic ocean (higher iron flux) but constituted up to half of all Prochlorococcus genomes from metagenomes in the N. Pacific (lower iron flux). Prochlorococcus siderophore consumers, like many other bacteria with this trait, also lack siderophore biosynthesis genes indicating that they scavenge exogenous siderophores from seawater. Statistical modeling suggests that the capacity for siderophore uptake is endemic to remote ocean regions where atmospheric iron fluxes are the smallest, particularly at deep chlorophyll maximum and primary nitrite maximum layers. We argue that abundant siderophore consumers at these two common oceanographic features could be a symptom of wider community iron stress, consistent with prior hypotheses. Our results provide a clear example of iron as a selective force driving the evolution of Prochlorococcus.

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Section: Data Sourcesmentioning

confidence: 99%

Section: Data Sourcesmentioning

confidence: 99%

Section: Data Sourcesmentioning

confidence: 99%

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Siderophores as an iron source for Prochlorococcus in deep chlorophyll maximum layers of the oligotrophic ocean

Hogle

Hackl

Bundy

et al. 2021

Preprint

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“…Thus, they are ubiquitous in terrestrial and aquatic ecosystems [10][11][12][13][14] . Phosphonates also represent a major fraction of the marine organic phosphorus pool 11,[15][16][17] and recent studies have now identified several cosmopolitan marine microorganisms capable of synthesising significant quantities of these compounds 6,7,9,18,19 . Collectively, this synthesis drives a vast global oceanic phosphorus redox cycle with reduced phosphorus input in the surface ocean estimated to be an order of magnitude greater than pre-anthropogenic riverine phosphorus input 9 .…”

Section: Introductionmentioning

confidence: 99%

“…However, emerging evidence suggests that Pi-insensitive 2AEP catabolism occurs in nature 22,23 . Notably, the absence of 2AEP from otherwise phosphonate rich high molecular weight (HMW) DOM 16,17 , despite its supposed ubiquitous production 9,19,24,25 , suggests preferential catabolism of this molecule in comparison to alkylphosphonates.…”

Section: Introductionmentioning

confidence: 99%

Aminophosphonate mineralisation is a major step in the global oceanic phosphorus redox cycle

Murphy¹,

Scanlan²,

Yin³

et al. 2020

Preprint

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The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Thus, we hypothesised unknown pathways for the uptake of aminophosphonates must exist in seawater. Here, we identify three novel bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose expression is independent of phosphate concentrations (phosphate-insensitive). AepXVW, is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphate-regulated phosphonate binding protein, PhnD, the newly identified AepX is heavily transcribed (~100-fold>PhnD) in the global ocean independently of phosphate and nitrogen concentrations. Collectively, our data identifies a mechanism responsible for the oxidative step in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate, which is required for oceanic primary production.

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Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle

et al. 2021

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The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Here, we identify three bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose synthesis is independent of phosphate concentrations (phosphate-insensitive). AepXVW is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphonate binding protein, PhnD, AepX has high affinity and high specificity for 2AEP (Stappia stellulata AepX Kd 23 ± 4 nM; methylphosphonate Kd 3.4 ± 0.3 mM). In the global ocean, aepX is heavily transcribed (~100-fold>phnD) independently of phosphate and nitrogen concentrations. Collectively, our data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production.

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Phosphonate production by marine microbes: exploring new sources and potential function

Cited by 9 publications

References 112 publications

Siderophores as an iron source for Prochlorococcus in deep chlorophyll maximum layers of the oligotrophic ocean

Siderophores as an iron source for Prochlorococcus in deep chlorophyll maximum layers of the oligotrophic ocean

Aminophosphonate mineralisation is a major step in the global oceanic phosphorus redox cycle

Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle

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