Phosphonate utilization by bacteria

Cook, Alasdair M.; Daughton, Christian G.; Alexander, Martin

doi:10.1128/jb.133.1.85-90.1978

Cited by 127 publications

(51 citation statements)

References 17 publications

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“…For the mobilization of Po, P mineralizing bacteria exude nonspecific phosphatase (phosphomonoesterase, phosphodiesterase, phosphotriesterase), phytase, phosphonatase or C-P lyase to hydrolyze Po and release orthophosphate (Eivazi and Tabatabai, 1977;Nannipieri et al, 2011). Some P mineralizing bacteria can also hydrolyze Po via C-P lyase enzymes (Cook et al, 1978;Quinn et al, 1989;Dyhrman et al, 2006). The genes encoding these phosphatases have been intensively characterized, for example, phoD, olpA, suhB, php, glpQ and phn (Bergkemper et al, 2016;Ragot et al, 2016;Luo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Contrasting P acquisition strategies of the bacterial communities associated with legume and grass in subtropical orchard soil

Yang

Zhu

Yao

2018

Environ Microbiol Rep

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Phosphorus (P) cycling is a fundamental process driven by microorganisms, and plants can regulate P cycling directly or via their influence on the soil microbial community. However, the differential P cycling patterns associated with legumes and grass are largely unknown. Therefore, we investigated the microbial community involved in P cycling in subtropical soil grown with stylo (Stylosanthes guianensis, legume) or bahiagrass (Paspalum notatum, grass) using metagenomic sequencing. P fractionation indicated that sparingly soluble inorganic P (Pi) accounted for approximately 75% of P pool. Bacteria involved in sparingly soluble Pi solubilization (pqq, gad, JEN) were more abundant in bahiagrass soil, with Candidatus Pelagibacter, Trichodesmium, Neorickettsia, Nitrobacter, Paraburkholderia, Candidatus Solibacter, Burkholderia as major contributors. In contrast, bacteria involved in organic P (Po) mineralization (php, glpQ, phn) were more abundant in stylo soil, consistent with phosphatase activity and Frankia, Kyrpidia, Thermobispora, Streptomyces, Rhodococcus were major contributors. Bacteria taking up low molecular-weight Po were more abundant in stylo soil than in bahiagrass soil, while those taking up Pi were less abundant. These data suggest that bacterial communities associated with legumes and grass develop contrasting P acquisition strategies, highlighting the possibility of intercropping with legumes and grass for better P cycling.

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Section: Introductionmentioning

confidence: 99%

Contrasting P acquisition strategies of the bacterial communities associated with legume and grass in subtropical orchard soil

Yang

Zhu

Yao

2018

Environ Microbiol Rep

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“…They showed that Escherichia coli could sustain growth on the synthetic organophosphonates, methylphosphonate and ethyl phosphonate, as sole sources of phosphorus. Since then, there have been a number of reports of bacteria utilizing organophosphonates as sole phosphorus sources (2-4, 8, 19) or as the sole carbon, nitrogen, or phosphorus source (3). LaNauze et al (14,15) were the first to find direct evidence for the enzymatic cleavage of a C-P bond.…”

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confidence: 99%

Isolation of a Pseudomonas sp. Which Utilizes the Phosphonate Herbicide Glyphosate

III

Braymer

Larson

1983

Appl Environ Microbiol

122

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A strain of bacteria has been isolated which rapidly and efficiently utilizes the herbicide glyphosate ( N -phosphonomethylglycine) as its sole phosphorus source in a synthetic medium. The strain (PG2982) was isolated by subculturing Pseudomonas aeruginosa ATCC 9027 in a synthetic broth medium containing glyphosate as the sole phosphorus source. Strain PG2982 differs from the culture of P. aeruginosa in that it is nonflagellated, does not produce pyocyanin, and has an absolute requirement for thiamine. Strain PG2982 has been tentatively identified as a Pseudomonas sp. strain by its biochemical activities and moles percent guanine plus cytosine. Measurements of glyphosate with an amino acid analyzer show that glyphosate rapidly disappears from the medium during exponential growth of strain PG2982. In batch culture at 30�C, this isolate completely utilized 1.0 mM glyphosate in 96 h and yielded a cell density equal to that obtained with 1.0 mM phosphate as the phosphorus source. However, a longer lag phase and greater generation time were noted in the glyphosate-containing medium. Strain PG2982 can efficiently utilize glyphosate as an alternate phosphorus source.

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“…Metabolism of AEP is not unique to ruminal bacteria. Cook et al (1978) isolated bacteria from sewage and soil that were capable of using different alkylphosphonates as phosphorus sources. One of the isolates, identified as Pseudomonas putida, grew with AEP as its sole carbon, nitrogen and phosphorus source and released nearly all of the organic phosphorus as orthophosphate and 72% of the AEP nitr0ge0 ~ as ammonia.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of 2-Aminoethylphosphonic Acid in Feeds, Ruminal Bacteria and Duodenal Digesta from Defaunated Sheep

Ankrah

Loerch

Dehority

1989

Journal of Animal Science

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A quantitative method of analysis for 2-aminoethylphosphonic acid (AEP) was developed using reverse-phase HPLC. The detection limit for AEP was 15 nM, and the detector response (peak area) was linear from AEP levels up to 100 microM (R = .99). Mean recovery of AEP added to strained ruminal fluid from faunated sheep was 98.2%. When AEP was added to a fermentation mixture at a concentration of 22.6 micrograms/ml, 78% disappeared during a 24-h incubation. 2-Aminoethylphosphonic acid was readily detected in preparations of mixed ruminal ciliate protozoa as well as in mixed and pure strains of ruminal bacteria, feedstuffs, and ruminal fluid and duodenal digesta from defaunated sheep. The occurrence of AEP in feed and bacterial hydrolysates was confirmed by organic phosphorus analyses. The concentration of AEP in mixed ruminal protozoa was three times greater than its concentration in mixed ruminal bacteria (4,304 vs 1,383 micrograms/g DM, respectively). The AEP values for pure ruminal bacterial cultures ranged from 733 micrograms/g DM in Bacteroides succinogenes B21a to 1,166 micrograms/g DM in Butyrivibrio fibrisolvens H17c. Ruminal fluid and duodenal digesta from defaunated sheep contained AEP concentrations of 30 micrograms/ml and 90 micrograms/g DM, respectively. The concentration of AEP in feedstuffs ranged from 25 micrograms/g DM in wheat straw to 263 micrograms/g DM in oats. Because AEP occurrence is not limited to ruminal ciliate protozoa, it is of little value as a marker for protozoal presence in or passage out of the rumen.

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Phosphonate utilization by bacteria

Cited by 127 publications

References 17 publications

Contrasting P acquisition strategies of the bacterial communities associated with legume and grass in subtropical orchard soil

Contrasting P acquisition strategies of the bacterial communities associated with legume and grass in subtropical orchard soil

Isolation of a Pseudomonas sp. Which Utilizes the Phosphonate Herbicide Glyphosate

Occurrence of 2-Aminoethylphosphonic Acid in Feeds, Ruminal Bacteria and Duodenal Digesta from Defaunated Sheep

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