Phosphoproteome analysis reveals chitosan-induced resistance to osmotic stress in rice (<i>Oryza sativa</i> L.) seedlings

Pongprayoon, Wasinee; Maksup, Sarunyaporn; Phaonakrop, Narumon; Jaresitthikunchai, Junthima; Uawisetwathana, Umaporn; Panya, Atikorn; Roytrakul, Sittiruk

doi:10.1080/17429145.2022.2114556

Cited by 10 publications

(4 citation statements)

References 91 publications

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“…As an adaptive strategy, salinity-responsive proteins are produced to counterbalance damaging impacts on sub-cellular processes and for the induction of stress tolerance through the readjustment of biochemical attributes [ 73 , 74 ]. The CS applications further boost the production of proteins and other important biomolecules under stress conditions [ 47 , 48 ]. CS-priming treatments induced an increase in TSP as a salinity-responsive strategy in rice seedlings [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Nanopriming-mediated memory imprints reduce salt toxicity in wheat seedlings by modulating physiobiochemical attributes

et al. 2022

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Background Around the globe, salinity is one of the serious environmental stresses which negatively affect rapid seed germination, uniform seedling establishment and plant developments restricting sustainable agricultural productivity. In recent years, the concepts of sustainable agriculture and cleaner production strategy have emphasized the introduction of greener agrochemicals using biocompatible and natural sources to maximize crop yield with minimum ecotoxicological effects. Over the last decade, the emergence of nanotechnology as a forefront of interdisciplinary science has introduced nanomaterials as fast-acting plant growth-promoting agents. Results Herein, we report the preparation of nanocomposite using chitosan and green tea (CS-GTE NC) as an ecofriendly nanopriming agent to elicit salt stress tolerance through priming imprints. The CS-GTE NC-primed (0.02, 0.04 and 0.06%), hydroprimed and non-primed (control) wheat seeds were germinated under normal and salt stress (150 mM NaCl) conditions. The seedlings developed from aforesaid seeds were used for physiological, biochemical and germination studies. The priming treatments increased protein contents (10–12%), photosynthetic pigments (Chl a (4–6%), Chl b (34–36%), Total Chl (7–14%) and upregulated the machinery of antioxidants (CAT (26–42%), POD (22–43%)) in wheat seedlings under stress conditions. It also reduced MDA contents (65–75%) and regulated ROS production resulting in improved membrane stability. The priming-mediated alterations in biochemical attributes resulted in improved final germination (20–22%), vigor (4–11%) and germination index (6–13%) under both conditions. It reduced mean germination time significantly, establishing the stress-insulating role of the nanocomposite. The improvement of germination parameters validated the stimulation of priming memory in composite-treated seeds. Conclusion Pre-treatment of seeds with nanocomposite enables them to counter salinity at the seedling development stage by means of priming memory warranting sustainable plant growth and high crop productivity.

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Section: Discussionmentioning

confidence: 99%

Nanopriming-mediated memory imprints reduce salt toxicity in wheat seedlings by modulating physiobiochemical attributes

et al. 2022

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“…The Chl a , Chl b , and carotenoid contents were measured following the methods of Pongprayoon et al [ 19 ]. Briefly, 200 mg of fresh leaves were homogenized with 5 mL of 80% ( v/v ) acetone, then wrapped in aluminum foil and placed in a refrigerator for 48 h. The Chl a and Chl b were quantified at wavelengths of 662 and 644 nm, whereas carotenoids were determined at 470 nm on a microplate spectrophotometer (Multiscan GO; Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The extraction procedure for phosphoproteomic analysis was conducted in accordance with the method described by Pongprayoon et al [ 19 ]. Approximately 0.5 g of fresh leaf tissue was ground into a fine powder in liquid nitrogen and resuspended in 900 mL of 0.5% ( w/v ) sodium dodecyl sulfate (SDS).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, phosphoproteomes of drought-sensitive rice cultivars, Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105), were investigated. LPT123 and KDML105 cultivars were reported to respond to CTS pretreatment before exposure to osmotic stress [ 18 , 19 ]. However, limited studies report the function of CTS in improving drought resistance and CTS-induced changes in phosphoproteins in LPT123.…”

Section: Introductionmentioning

confidence: 99%

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Phosphoprotein Profile of Rice (Oryza sativa L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan

Pongprayoon

Panya

Jaresitthikunchai

et al. 2022

Plants

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This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.

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Plant-Environment Interactions: Proteomics, Metabolomics and Genetic Engineering Perspective

Desa,

Saidin,

Azeyanty

et al. 2023

Environmental Science and Engineering

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Phosphoproteome analysis reveals chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.) seedlings

Cited by 10 publications

References 91 publications

Nanopriming-mediated memory imprints reduce salt toxicity in wheat seedlings by modulating physiobiochemical attributes

Nanopriming-mediated memory imprints reduce salt toxicity in wheat seedlings by modulating physiobiochemical attributes

Phosphoprotein Profile of Rice (Oryza sativa L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan

Plant-Environment Interactions: Proteomics, Metabolomics and Genetic Engineering Perspective

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