Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus

Abstract

Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to virus infection. In this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (Zea mays L.) in response to Sugarcane mosaic virus (SCMV) infection. Based on isotope tags for relative and absolute quantification technology, TiO2 enrichment method and LC-MS/MS analysis, we identified 65 and 59 phosphoproteins respectively, whose phosphorylation level regulated significantly in susceptible and resistant plants. Some identified phosphoproteins were shared by both genotypes, suggesting a partial overlapping of the responsive pathways to virus infection. While several phosphoproteins are well-known pathogen response phosphoproteins, virus infection differentially regulates most other phosphoproteins, which has not been reported in literature. Changes in protein phosphorylation status indicated that response to SCMV infection encompass a reformatting of major cellular processes. Our data provide new valuable insights into plant-virus interactions.

Quantitative analysis of changes in the phosphoproteome of maize induced by the plant hormone salicylic acid

“…Ferritin gene expression is modulated by various environmental factors, including the intensity of drought, cold, light and pathogenic attack 41 . It has also been reported that the phosphorylation level of ferritin was significantly changed under virus infection in maize, which indicated that the phosphorylation of ferredoxin might play a positive roles in maize for the resistance to pathogen infection 33 .…”

“…The expression of SR protein under different environmental cues suggests they have an important role in plant defense and HR-like cell death 32 . It has been reported that the phosphorylation level of arginine SR splicing factor was significantly changed both under biotic and abiotic stresses 26 33 . In this study, the phosphorylation level of the arginine SR splicing factor was relatively stable from 1 to 4 h after SA treatment, but from 4 to 9 h, there was a sharp increase ( Fig.…”

“…It is also responsible for rapid adjustments in plant growth patterns under environmental changes 40 . Phosphorylation levels of two ribosomal proteins were differentially regulated in maize after virus infection, with 40S ribosomal protein up-regulated, and 60S ribosomal protein down-regulated 33 . In this study, the phosphorylation level of bZIP transcription factor showed a trend similar to that of the 40S ribosomal protein, increasing from 1 to 4 h and declining from 4 to 9 h after SA treatment.…”

The Genetics and Genomics of Virus Resistance in Maize

“…For example, the large subunit of RuBisCO accumulated in resistant maize inoculated with SCMV (Wu et al 2013a;Wu et al 2015). Chloroplast localized ferredoxin V and thioredoxins was also upregulated (Cao et al 2012;Cheng et al 2008;Wu et al 2015). Taken together with interactions of SCMV viral proteins with the RuBisCo small subunit and ferredoxin (Cheng et al 2008;Leng et al 2017), the results suggest an intimate relationship between photosynthetic activity and potyvirus infection.…”

“…Increased expression of transcripts for genes important for photosynthesis has been noted in some systems. For example, the large subunit of RuBisCO accumulated in resistant maize inoculated with SCMV (Wu et al 2013a;Wu et al 2015). Chloroplast localized ferredoxin V and thioredoxins was also upregulated (Cao et al 2012;Cheng et al 2008;Wu et al 2015).…”