Phosphoric acid concentration affects dentinal MMPs activity

DeVito-Moraes, André Guaraci; Francci, Carlos Eduardo; Vidal, Cristina; Scaffa, Polliana Mendes Cándia; Nesadal, Douglas; Yamasaki, L.C.; Nicolau, José Carlos; Nascimento, Fábio D.; Pashley, David H.; Carrilho, Marcela Rocha de Oliveira

doi:10.1016/j.jdent.2016.06.002

Cited by 32 publications

(19 citation statements)

References 36 publications

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“…The control groups (phosphoric and maleic) depicted the highest enzymatic activity while glycolic had the lowest level of gelatinolytic activation of demineralized dentin powder. Several studies have demonstrated that activation and functionality of dentin MMPs are very much dependent on pH [42][43][44][45]. Indeed, our results are consistent with previous findings, since control groups exhibited the lowest pH value and the maximum enzymatic activity.…”

Section: Discussionsupporting

confidence: 93%

“…Among the control groups, phosphoric exhibited the lowest enzymatic activity and pH, although not statistically significant. An interestingly observation was that phosphoric acid reduced the inherent dentin gelatinolytic activity until 14 h. It was shown that higher concentrations of phosphoric (35% -40%), exhibiting a pH as low as 0.4, elicit transient MMPs inactivation [43,45,46]. In contrast, maleic displayed an average pH of 0.8 and kept the highest enzymatic activity of dentin powder along the 24 h incubation period.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of five α-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity

et al. 2019

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Objectives: Surface conditioning of enamel and dentin is a key step during adhesive restorative procedures and strategies. The aim of this study was to investigate the effectiveness of five αhydroxy-acids (AHAs) as enamel and dentin surface etchants. Methods: Enamel and dentin specimens were prepared from human molars to determine the depth of demineralization by optical profilometry (Δz), the resin bond strength to enamel and dentin (μTBS), the micro-permeability of dentin-resin interfaces, and the gelatinolytic activity of dentin matrix induced by AHAs [glycolic (GA), lactic (LA), citric (CA), malic (MI) and tartaric (TA)] and controls [phosphoric (PA) and maleic (MA)]. All acids were prepared at 35% concentration. Adhesion studies employed Adper Single Bond Plus bonding system. Data were individually processed and analyzed by ANOVA, post-hoc tests and Pearson correlations (α=0.05). Results: AHA exhibited statistically lower depth of demineralization of enamel and dentin (average 4 fold) than controls (p<0.001). In enamel, MA and PA etching resulted in higher μTBS than AHA groups (p˂0.001). In dentin, GA, TA, CI and LA etching resulted in statistically similar μTBS than PA (p<0.05). The hybrid-layer (HL) thickness and interfacial micro-permeability intensity were statistically lower for AHA groups (p<0.05). A significant positive correlation was observed between the intensity of micro-permeability and the thickness of HL (p<0.05). AHA etchants elicited lower dentin enzymatic activity than controls (p<0.05).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Investigation of five α-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity

et al. 2019

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“…All discs of dentin (SD and CAD) were separated and frozen at −80°C for 24 hr (DeVito‐Moraes et al, 2016) and then powdered by a ball mill (MM400, Retsch GmbH, Haan, DE). The powdered dentin was stored frozen in tubes until needed.…”

Section: Methodsmentioning

confidence: 99%

Effects of a biomimetic analog‐based experimental bonding system on caries‐affected and sound dentin

Gomes

Rossi

Silva

et al. 2020

Microscopy Res & Technique

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This study compared the ultrastructure, chemical composition, and proteases activity (PA) of sound (SD) and caries-affected dentin (CAD) in the dentin hybrid layer after using an experimental bonding system containing pyromellitic dianhydride glycerol methacrylate and biomimetic analogs. The bonding system used a three step and a total-etch procedure. Polyacrylic acid (5%) and sodium trimetaphosphate (5%) were added to the primer and monocalcium phosphate monohydrate (9%), beta-tricalcium phosphate (10.5%), and calcium hydroxide (0.5%) were added to the adhesive. Transmission electron microscopy (TEM) was used to evaluate the resultant structure, particularly the adhesive-dentin and the demineralized-SD interfaces. The chemical composition was evaluated through energy-dispersive X-ray spectroscopy (EDS) and selected area electron diffraction (SAED). The PA was measured with the Coomassie Blue-G250 coloring test, and the PA data were analyzed by ANOVA. EDS identified the presence of isolated calcium phosphate nanoparticles in the demineralized region; however, the SAED analysis did not show any evidences of hydroxyapatite (HA) neoformation in SD and CAD. The biomimetic analog-based adhesive system inhibited the activities of dentin proteases immediately after treatment. Additionally, the proteolytic activity on the affected dentin resembled that of the SD. In conclusion, no HA formed in the demineralized SD and CAD although there were calcium and phosphate deposits. The experimental adhesive system inhibited dentin proteases. • The present study uses a new approach to investigate the hybrid layer behavior in dentin. • The experimental adhesive system was synthesized and used on sound and affected-caries dentin as the substrate to reproduce real clinical conditions.

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“…1 Demineralization of dental substrates occurs mainly by acids from diet (foods and drinks), 1 bacteria, 4 or dental restorative procedures such as acid etching. 5,6 Despite the high mineral concentration (~96 wt.%), enamel has a very low organic content (~1 wt.%) predominantly concentrated in the inner region of this substrate. 7 The post-eruptive enamel organic matrix contains little percent of type IV and VII collagen, proteolyzed fragments and matrix metalloproteinase (MMP)-20 7 also known as enamelysin.…”

Section: Introductionmentioning

confidence: 99%

Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Scheffel

Cury

Tenuta

et al. 2020

Int J Paed Dentistry

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Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates.

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Phosphoric acid concentration affects dentinal MMPs activity

Cited by 32 publications

References 36 publications

Investigation of five α-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity

Investigation of five α-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity

Effects of a biomimetic analog‐based experimental bonding system on caries‐affected and sound dentin

Proteolytic activity, degradation, and dissolution of primary and permanent teeth

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