Phosphorus and Nitrogen Limitation as a Part of the Strategy to Stimulate Microbial Lipid Biosynthesis

Wierzchowska, Katarzyna; Zieniuk, Bartłomiej; Nowak, Dorota; Fabiszewska, Agata

doi:10.3390/app112411819

Cited by 30 publications

(21 citation statements)

References 49 publications

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“…For the described purposes, especially Yarrowia lipolytica yeast can be used due to its ability to grow in media with various energy and carbon waste sources. The yeast use hydrophobic substrates, e.g., waste oils and other lipid-rich materials [11][12][13]. In recent years, many efforts have been made to produce microbial lipids using waste materials as substrates to lower cost of the biotechnological production.…”

Section: Introductionmentioning

confidence: 99%

Brine and Post-Frying Oil Management in the Fish Processing Industry—A Concept Based on Oleaginous Yeast Culture

et al. 2022

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Waste management solutions including the valorization of waste materials in biotechnological processes is an important issue needing to be explored. A significant amount of waste is being generated by the food industry. In this study, an attempt was made to utilize two fish industry wastes simultaneously—waste brine and post-frying oil from frying fish fillets in Yarrowia lipolytica culture with high single cell oil synthesis yield. Oxygenation in the culture medium had a positive effect on the biosynthesis efficiency of microbial oil, resulting in the highest content of lipids in yeast cells at the level of 0.431 g/g dm (dry mass). Y. lipolytica yeast preferentially accumulated oleic acid and linoleic acid, and the high content of linolenic acid, valuable from a nutritional point of view, was also found in microbial oil. This study proved that the use of post-frying rapeseed oil gives a chance to obtain valuable storage lipids in Y. lipolytica yeast cells via ex novo biosynthesis pathway. Furthermore, the wastewater stream could be limited using a waste brine as a solvent in medium preparation, but the brine share should not exceed 30% so as not to inhibit yeast cell growth.

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Section: Introductionmentioning

confidence: 99%

Brine and Post-Frying Oil Management in the Fish Processing Industry—A Concept Based on Oleaginous Yeast Culture

et al. 2022

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“…To screen for oleaginous yeast strains, all the yeast strains were determined for their lipid accumulation in a limited nitrogen source condition using 2G2M medium containing 20 g L −1 glucose and 20 g L −1 malt extract, which was modified from the medium used by Yamazaki et al [ 43 ]. The cultivation of yeast in nitrogen-limited medium has been reported to promote lipid accumulation of the oleaginous yeast strains [ 10 , 11 , 33 , 36 , 44 ]. To prepare an inoculum, one loopful of isolated yeast strain was inoculated in 50 mL of YM broth in a 250 mL Erlenmeyer flask and incubated on a rotary shaker (ZWYR-211C Labwit, Victoria, Australia) at 30 °C, 150 rpm for 24 h. Cells were collected by centrifugation (3700 Kubota, Tokyo Japan) at 4 °C, 5000 rpm, 10 min, washed with sterile normal saline solution, and adjusted to OD 1 at 600 nm.…”

Section: Methodsmentioning

confidence: 99%

Discovery of Oleaginous Yeast from Mountain Forest Soil in Thailand

Sapsirisuk

Polburee

Lorliam

et al. 2022

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As an interesting alternative microbial platform for the sustainable synthesis of oleochemical building blocks and biofuels, oleaginous yeasts are increasing in both quantity and diversity. In this study, oleaginous yeast species from northern Thailand were discovered to add to the topology. A total of 127 yeast strains were isolated from 22 forest soil samples collected from mountainous areas. They were identified by an analysis of the D1/D2 domain of the large subunit rRNA (LSU rRNA) gene sequences to be 13 species. The most frequently isolated species were Lipomyces tetrasporus and Lipomyces starkeyi. Based on the cellular lipid content determination, 78 strains of ten yeast species, and two potential new yeast that which accumulated over 20% of dry biomass, were found to be oleaginous yeast strains. Among the oleaginous species detected, Papiliotrema terrestris and Papiliotrema flavescens have never been reported as oleaginous yeast before. In addition, none of the species in the genera Piskurozyma and Hannaella were found to be oleaginous yeast. L. tetrasporus SWU-NGP 2-5 accumulated the highest lipid content of 74.26% dry biomass, whereas Lipomyces mesembrius SWU-NGP 14-6 revealed the highest lipid quantity at 5.20 ± 0.03 g L−1. The fatty acid profiles of the selected oleaginous yeasts varied depending on the strain and suitability for biodiesel production.

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“…Parameters for yeast cultures were calculated according to Fabiszewska et al [16]. Culture conditions were chosen on the basis of our previous researches [4,9,16].…”

Section: Culture Conditionsmentioning

confidence: 99%

“…Sci. 2022, 12, 12877 2 of 14 Y. lipolytica make the species able to utilize both simple and complex wastes as sources of carbon, nitrogen, phosphorus and other essential components [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the ability of oleaginous Y. lipolytica cells to grow and accumulate significant amounts of microbial lipids in media with hydrophobic carbon sources, there was established, that waste cooking oils (WCOs) may be considered as an efficient source of carbon stimulating lipase activity and promoting the Single Cell Oils (SCOs) accumulation process [9,12,13]. In the food industry, microbial oils have gained popularity as additives in infant formula and animal feed.…”

Section: Introductionmentioning

confidence: 99%

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Concept of Batch and Fed-Batch Cultures of Yarrowia lipolytica as a Valuable Source of Sterols with Simultaneous Valorization of Molasses and Post-Frying Rapeseed Oil

et al. 2022

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Food byproduct streams can potentially be transformed into value-added products such as microbial lipids in bioprocesses based on the non-conventional Yarrowia yeast. The effect of culture conditions of Y. lipolytica KKP 379 wild strain in waste media on the efficiency of lipid accumulation, fatty acid composition, presence of selected sterols, yield and elemental composition of biomass has been studied. Batch and fed-batch bioreactor cultures were carried out in media with molasses hydrolysate (MH) and post-frying rapeseed oil. It was determined that biomass grown in MH contained more minerals than in medium with rapeseed post-frying oil. Considering the PDSC study, the Tmax of oxidation induction ranged from 10.04–26.36 min for the analyzed samples. The biomass from fed-batch cultures with MH had the highest total sterol content (68.40 mg/goil), dominated by ergosterol at 60.16 mg/g. Feeding with post-frying rapeseed oil with new doses of mineral medium promoted maintaining the cellular lipid content at a high level (30.75–31.73%) for 50 h, with maximum yield at 37.50%. The results of the experiment showed that the cellular lipid accumulation efficiency of Y. lipolytica yeast and the content of sterols in the cell membrane can be manipulated by selecting waste substrates and culture mode.

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Phosphorus and Nitrogen Limitation as a Part of the Strategy to Stimulate Microbial Lipid Biosynthesis

Cited by 30 publications

References 49 publications

Brine and Post-Frying Oil Management in the Fish Processing Industry—A Concept Based on Oleaginous Yeast Culture

Brine and Post-Frying Oil Management in the Fish Processing Industry—A Concept Based on Oleaginous Yeast Culture

Discovery of Oleaginous Yeast from Mountain Forest Soil in Thailand

Concept of Batch and Fed-Batch Cultures of Yarrowia lipolytica as a Valuable Source of Sterols with Simultaneous Valorization of Molasses and Post-Frying Rapeseed Oil

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