Phosphorylation of chitosan to improve osteoinduction of chitosan/xanthan-based scaffolds for periosteal tissue engineering

Souza, Renata Francielle Bombaldi de; Souza, Fernanda Carla Bombaldi de; Thorpe, Andrea S.; Mantovani, Diego; Popat, Ketul C.; Moraes, Ângela Maria

doi:10.1016/j.ijbiomac.2019.12.004

Cited by 76 publications

(31 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ALP is an enzyme present in bone-related cells and is considered key to mineralization [ 74 ]. Its activity is related to the level of inorganic phosphate, a component of the bone mineral phase [ 75 ]. Therefore, ALP activity has been considered as an early indicator of osteoblast differentiation.…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma

Haseli

Castilla‐Casadiego

Pinzón-Herrera

et al. 2022

Materials Today Bio

View full text Add to dashboard Cite

Human mesenchymal stromal cells (hMSCs) are multipotent cells that have been proposed for cell therapies due to their immunosuppressive capacity that can be enhanced in the presence of interferon-gamma (IFN-γ). In this study, multilayers of heparin (HEP) and collagen (COL) (HEP/COL) were used as a bioactive surface to enhance the immunomodulatory activity of hMSCs using soluble IFN-γ. Multilayers were formed, via layer-by-layer assembly, varying the final layer between COL and HEP and supplemented with IFN-γ in the culture medium. We evaluated the viability, adhesion, real-time growth, differentiation, and immunomodulatory activity of hMSCs on (HEP/COL) multilayers. HMSCs viability, adhesion, and growth were superior when cultured on (HEP/COL) multilayers compared to tissue culture plastic. We also confirmed that hMSCs osteogenic and adipogenic differentiation remained unaffected when cultured in (HEP/COL) multilayers in the presence of IFN-γ. We measured the immunomodulatory activity of hMSCs by measuring the level of indoleamine 2,3-dioxygenase (IDO) expression. IDO expression was higher on (HEP/COL) multilayers treated with IFN-γ. Lastly, we evaluated the suppression of peripheral blood mononuclear cell (PBMC) proliferation when co-cultured with hMSCs on (HEP/COL) multilayers with IFN-γ. hMSCs cultured in (HEP/COL) multilayers in the presence of soluble IFN-γ have a greater capacity to suppress PBMC proliferation. Altogether, (HEP/COL) multilayers with IFN-γ in culture medium provides a potent means of enhancing and sustaining immunomodulatory activity to control hMSCs immunomodulation.

show abstract

Section: Resultsmentioning

confidence: 99%

Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma

Haseli

Castilla‐Casadiego

Pinzón-Herrera

et al. 2022

Materials Today Bio

View full text Add to dashboard Cite

show abstract

“…Pectin is highly available, biodegradable, and non-toxic, which makes it a potential material for biomedical applications [ 22 , 23 ]. Moreover, the mixture of chitosan and pectin forms a polyelectrolyte complex mainly conformed by electrostatic interactions between the amino groups of chitosan and the carboxylic groups of pectin [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

3D Printed Chitosan-Pectin Hydrogels: From Rheological Characterization to Scaffold Development and Assessment

Zarandona

Bengoechea

Álvarez‐Castillo

et al. 2021

Gels

View full text Add to dashboard Cite

Chitosan-pectin hydrogels were prepared, and their rheological properties were assessed in order to select the best system to develop scaffolds by 3D printing. Hydrogels showed a weak gel behavior with shear thinning flow properties, caused by the physical interactions formed between both polysaccharides, as observed by FTIR analysis. Since systems with high concentration of pectin showed aggregations, the system composed of 2 wt% chitosan and 2 wt% pectin (CHI2PEC2) was selected for 3D printing. 3D printed scaffolds showed good shape accuracy, and SEM and XRD analyses revealed a homogeneous and amorphous structure. Moreover, scaffolds were stable and kept their shape and size after a cycle of compression sweeps. Their integrity was also maintained after immersion in PBS at 37 °C, showing a high swelling capacity, suitable for exudate absorption in wound healing applications.

show abstract

“…XAN has been used for clinical applications because of its excellent biocompatibility, biodegradability, nontoxicity, resistivity toward mechanical degradability, and stability over a broad range of pH and temperature. Apart from these, properties that have attracted our attention toward the harnessing of XAN as an anionic counterpart in the polyelectrolyte matrix include the presence of a large number of carboxylic groups in XAN which allows its interaction with calcium and thus serves as nucleation points for hydroxyapatite mineralization which in turn is known to promote the osteoinduction of implants . XAN has also been reported to mimic the environment of the extracellular matrix (ECM) because of its high liquid retention ability.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite Nanoparticles Fortified Xanthan Gum–Chitosan Based Polyelectrolyte Complex Scaffolds for Supporting the Osteo-Friendly Environment

Zia

Jolly

Mirza

et al. 2020

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Nanoparticle-reinforced polymer-based scaffolding matrices as artificial bone-implant materials are potential suitors for bone regenerative medicine as they simulate the native bone. In the present work, a series of bioinspired, osteoconductive tricomposite scaffolds made up of nanohydroxyapatite (NHA) embedded xanthan gum−chitosan (XAN−CHI) polyelectrolyte complex (PEC) are explored for their bone-regeneration potential. The Fourier transform infrared spectroscopy studies confirmed complex formation between XAN and CHI and showed strong interactions between the NHA and PEC matrix. The X-ray diffraction studies indicated regulation of the nanocomposite (NC) scaffold crystallinity by the physical cues of the PEC matrix. Further results exhibited that the XAN−CHI/NHA5 scaffold, with a 50/50 (polymer/NHA) ratio, has optimized porous structure, appropriate compressive properties, and sufficient swelling ability with slower degradation rates, which are far better than those of CHI/NHA and other XAN−CHI/NHA NC scaffolds. The simulated body fluid studies showed XAN− CHI/NHA5 generated apatite-like surface structures of a Ca/P ratio ∼1.66. Also, the in vitro cell−material interaction studies with MG-63 cells revealed that relative to the CHI/NHA NC scaffold, the cellular viability, attachment, and proliferation were better on XAN−CHI/NHA scaffold surfaces, with XAN−CHI/ NHA5 specimens exhibiting an effective increment in cell spreading capacity compared to XAN−CHI/NHA4 and XAN−CHI/ NHA6 specimens. The presence of an osteo-friendly environment is also indicated by enhanced alkaline phosphatase expression and protein adsorption ability. The higher expression of extracellular matrix proteins, such as osteocalcin and osteopontin, finally validated the induction of differentiation of MG-63 cells by tricomposite scaffolds. In summary, this study demonstrates that the formation of PEC between XAN and CHI and incorporation of NHA in XAN−CHI PEC developed tricomposite scaffolds with robust potential for use in bone regeneration applications.

show abstract

Phosphorylation of chitosan to improve osteoinduction of chitosan/xanthan-based scaffolds for periosteal tissue engineering

Cited by 76 publications

References 49 publications

Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma

Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma

3D Printed Chitosan-Pectin Hydrogels: From Rheological Characterization to Scaffold Development and Assessment

Hydroxyapatite Nanoparticles Fortified Xanthan Gum–Chitosan Based Polyelectrolyte Complex Scaffolds for Supporting the Osteo-Friendly Environment

Contact Info

Product

Resources

About