Phosphorylation of dolichol by insect enzymes

Allué, Luis Alberto Quesada

doi:10.1016/0014-5793(79)80089-7

FEBS Letters

1979

DOI: 10.1016/0014-5793(79)80089-7

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Phosphorylation of dolichol by insect enzymes

Luis Alberto Quesada Allué¹

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1979

1989

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Cited by 12 publications

References 23 publications

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Dolichol phosphorylation occurs via a CTP‐dependent reaction in Artemia larvae

Horst

1989

J. Exp. Zool.

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The phosphorylation of dolichol in larval stages of the brine shrimp, Artemia salina, has been investigated. The dolichol kinase has been assayed in crude microsomes; the enzyme requires CTP as phosphoryl donor and calcium as divalent cation. Activity increases with both incubation time and added microsomal protein. The product of the reaction has been characterized by chromatographic and enzymatic procedures. With gamma-32P CTP as substrate, the apparent Km for CTP is 24 microM. Enzymatic activity is stimulated fivefold by exogenous dolichol. The specific activity of the enzyme increases with the frequency of molting. Dolichol kinase activity was detectable in membranes prepared from dormant Artemia cysts. The low level in dormancy may anticipate the critical role of the enzyme during hatching.

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Dolichol phosphorylation occurs via a CTP‐dependent reaction in Artemia larvae

Horst

1989

J. Exp. Zool.

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Lipid-bound sugars in malignant human breast tumors

1983

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Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl- and glucosyl-transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described.

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A differentiation-dependend polyisoprenol kinase in Dictyostelium discoideum

1982

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Crude membrane fractions of Dictyostelium discoideum show the capacity to synthesize (1--3H)dolicholphosphate from (1--3H)dolichol. Formation of dolicholphosphate increased continuously over the first 15 min. The reaction rate was nearly linear with respect to the dolichol content up to 150 microM. The phosphate donor for the reaction is CTP. The optimum concentration of CTP is about 0,75 mM. The reaction is dependent on divalent metal ions, magnesium being more effective than calcium or manganese. The activity of the polyisoprenol kinase depends on the course of the early development. Maximum enzyme activities are present 4--6 h after the induction of the differentiation.

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Phosphorylation of dolichol by insect enzymes

Cited by 12 publications

References 23 publications

Dolichol phosphorylation occurs via a CTP‐dependent reaction in Artemia larvae

Dolichol phosphorylation occurs via a CTP‐dependent reaction in Artemia larvae

Lipid-bound sugars in malignant human breast tumors

A differentiation-dependend polyisoprenol kinase in Dictyostelium discoideum

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