Phosphorylation of OsRbohB by the protein kinase OsDMI3 promotes H2O2 production to potentiate ABA responses in rice

Wang, Qingwen; Shen, Tao; Ni, Lan; Chen, Chao; Jiang, Jingjing; Cui, Zhenzhen; Wang, Shuang; Xu, Fengjuan; Yan, Runjiao; Jiang, Mingyi

doi:10.1016/j.molp.2023.04.003

Cited by 30 publications

(19 citation statements)

References 76 publications

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“…Plasma membrane‐localized RBOHs are thought to be “the engines behind ROS signaling,” as ROS produced by these RBOHs regulate many different stress responses in plants (Miller et al, 2009). The binding of Ca 2+ or phosphatidic acid as well as other modifications such as phosphorylation, persulfidation, and S ‐nitrosylation greatly influence RBOH‐mediated ROS production and the formation of ROS signatures in the apoplast, which may act as signaling molecules to transduce extracellular signals into cells (Gao et al, 2022; Wang et al, 2023). The roles of various receptor‐like kinases in mediating signaling from extracellular ROS have been revealed.…”

Section: Ros Signalingmentioning

confidence: 99%

“…As RBOH‐mediated ROS production plays a vital role in drought stress tolerance by inducing stomatal closure, RBOH activity must be tightly regulated in plants. The calmodulin (CaM)‐dependent protein kinase OsDMI3 (DOESN'T MAKE INFECTION S3) was recently shown to phosphorylate OsRBOHB and promote H 2 O 2 production to potentiate ABA signaling and drought stress tolerance in rice (Wang et al, 2023) (Figure 6). The important role of NADPH oxidases in plant drought stress responses was confirmed by another recent study showing that natural variation Similar to RCD1 One 1d ( ZmSRO1d ) confers drought tolerance in maize by promoting stomatal closure by stimulating ZmRBOHC activity and ROS production in guard cells (Gao et al, 2022) (Figure 6).…”

Section: Roles Of Ros In Plant Responses To Abiotic Stressmentioning

confidence: 99%

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Reactive oxygen species: Multidimensional regulators of plant adaptation to abiotic stress and development

Wang,

Liu,

Han

et al. 2024

JIPB

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Reactive oxygen species (ROS) are produced as undesirable by‐products of metabolism in various cellular compartments, especially in response to unfavorable environmental conditions, throughout the life cycle of plants. Stress‐induced ROS production disrupts normal cellular function and leads to oxidative damage. To cope with excessive ROS, plants are equipped with a sophisticated antioxidative defense system consisting of enzymatic and non‐enzymatic components that scavenge ROS or inhibit their harmful effects on biomolecules. Nonetheless, when maintained at relatively low levels, ROS act as signaling molecules that regulate plant growth, development, and adaptation to adverse conditions. Here, we provide an overview of current approaches for detecting ROS. We also discuss recent advances in understanding ROS signaling, ROS metabolism, and the roles of ROS in plant growth and responses to various abiotic stresses.This article is protected by copyright. All rights reserved.

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Section: Ros Signalingmentioning

confidence: 99%

Section: Roles Of Ros In Plant Responses To Abiotic Stressmentioning

confidence: 99%

Reactive oxygen species: Multidimensional regulators of plant adaptation to abiotic stress and development

Wang,

Liu,

Han

et al. 2024

JIPB

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“…Ca 2+ signal also activates RBOHs through protein phosphorylation mediated by Ca 2+ ‐dependent kinases like CDPKs and Ca 2+ /CaM‐dependent protein kinases (CCaMKs). In rice, the CCaMK, also known as doesn't make infections 3 (OsDMI3), phosphorylates OsRbohD at Ser‐191 to activate its NADPH oxidase activity for ROS generation (Wang, Shen, et al, 2023). Knockout of RbohD or application of inhibitors to eliminate ROS accumulation results in decreased Ca 2+ spiking; meanwhile, stress‐induced ROS production can be severely attenuated by treatment with Ca 2+ channel blockers (Ranf et al, 2011).…”

Section: Cam/cml‐mediated Regulation Of Ros Homeostasismentioning

confidence: 99%

Calmodulin and calmodulin‐like protein‐mediated plant responses to biotic stresses

Zeng

Zhu

Yuan

et al. 2023

Plant Cell & Environment

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Plants have evolved a set of finely regulated mechanisms to respond to various biotic stresses. Transient changes in intracellular calcium (Ca2+) concentration have been well documented to act as cellular signals in coupling environmental stimuli to appropriate physiological responses with astonishing accuracy and specificity in plants. Calmodulins (CaMs) and calmodulin‐like proteins (CMLs) are extensively characterized as important classes of Ca2+ sensors. The spatial‐temporal coordination between Ca2+ transients, CaMs/CMLs and their target proteins is critical for plant responses to environmental stresses. Ca2+‐loaded CaMs/CMLs interact with and regulate a broad spectrum of target proteins, such as ion transporters (including channels, pumps, and antiporters), transcription factors, protein kinases, protein phosphatases, metabolic enzymes and proteins with unknown biological functions. This review focuses on mechanisms underlying how CaMs/CMLs are involved in the regulation of plant responses to diverse biotic stresses including pathogen infections and herbivore attacks. Recent discoveries of crucial functions of CaMs/CMLs and their target proteins in biotic stress resistance revealed through physiological, molecular, biochemical, and genetic analyses have been described, and intriguing insights into the CaM/CML‐mediated regulatory network are proposed. Perspectives for future directions in understanding CaM/CML‐mediated signalling pathways in plant responses to biotic stresses are discussed. The application of accumulated knowledge of CaM/CML‐mediated signalling in biotic stress responses into crop cultivation would improve crop resistance to various biotic stresses and safeguard our food production in the future.

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“…AtRbohC, AtRbohH, and AtRbohJ are involved in the polar growth of root hair through auxin regulation of ROS homeostasis (Mangano et al, 2017) . In rice, OsRbohB promotes apoplastic H 2 O 2 production in response to abiotic stress (Shi et al, 2020; Wang et al, 2023) . Mutation of different OsRbohs leads to decreased rice blast, bacterial blight, and sheath blight resistance, and all osrboh mutants display one or more compromised yield traits (Zhu et al, 2024) .…”

Section: Introductionmentioning

confidence: 99%

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

Shen,

Wang,

Chen

et al. 2024

Preprint

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In plants, reactive oxygen species maintain strictly low intracellular levels that is prerequisite for their function as second messengers. However, abiotic and biotic stresses are also long-term processes, requiring rapid scavenging of excess intracellular ROS. Plant Class III peroxidases, as multifunctional enzymes, are core enzymes in regulating intracellular ROS homeostasis and are key to regulating the complicated signaling network. Here, we found a rice Class III peroxidase OsPrx20 maintains intracellular ROS homeostasis in different conditions. OsPrx20 is a target of Ca2+/calmodulin-dependent protein kinase (OsDMI3) and phosphorylates its Thr-244 site by OsDMI3. Overexpression of OsPrx20 enhances osmotic stress tolerance but reduces blast resistance, while lack of OsPrx20 is opposite, suggesting OsPrx20 positively regulates osmotic stress tolerance but negatively regulates blast resistance in rice. Meanwhile, overexpression of OsPrx20 enlarges spike size and grain fullness, whereas lack of OsPrx20 leads to dwarfism and smaller spike. In ABA signaling, OsPrx20 Thr-244 phosphorylation is specifically dependent on OsDMI3 to reduce the sensitivity of ABA to seed germination and root growth and to enhance osmotic stress tolerance without affecting spike and grain development. Our study reveals an essential regulatory mechanism that directly activates OsPrx20 in ABA signaling, highlighting the multi-functionality of OsPrx20 in different biological processes.

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Phosphorylation of OsRbohB by the protein kinase OsDMI3 promotes H2O2 production to potentiate ABA responses in rice

Cited by 30 publications

References 76 publications

Reactive oxygen species: Multidimensional regulators of plant adaptation to abiotic stress and development

Reactive oxygen species: Multidimensional regulators of plant adaptation to abiotic stress and development

Calmodulin and calmodulin‐like protein‐mediated plant responses to biotic stresses

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

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