Phosphorylation of PSII proteins in maize thylakoids in the presence of Pb ions

Romanowska, Elżbieta; Wasilewska, Wioleta; Fristedt, Rikard; Vener, Alexander V.; Zienkiewicz, Maksymilian

doi:10.1016/j.jplph.2011.10.006

Cited by 28 publications

(7 citation statements)

References 58 publications

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“…The mechanism of inhibitory action of Pb(NO 3 ) 2 and Pb(CH 3 CHOO) 2 consists in the formation of complexes between Pb 2+ and amino acid residues in photosynthetic proteins as was confirmed by fluorescence spectroscopy. Our results are in a good accordance with former findings of Miles et al (1972), Rashid and Popovic (1990), Prasad et al (1991) and Romanowska et al (2012). …”

Section: Resultssupporting

confidence: 94%

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Effect of Pb2+ ions on photosynthetic apparatus

Šeršeň¹,

Kráľová²,

Peško³

et al. 2014

gpb

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Abstract. Using model lead compounds Pb(NO 3 ) 2 and Pb(CH 3 CHOO) 2 the mechanism and the site of action of Pb 2+ ions in the photosynthetic apparatus of spinach chloroplasts were studied. Both compounds inhibited photosynthetic electron transport (PET) through photosystem 1 (PS1) and photosystem 2 (PS2), while Pb(NO 3 ) 2 was found to be more effective PET inhibitor. Using EPR spectroscopy the following sites of Pb 2+ action in the photosynthetic apparatus were determined: the water-splitting complex and the Z• /D • intermediates on the donor side of PS2 and probably also the ferredoxin on the acceptor side of PS1, because cyclic electron flow in chloroplasts was impaired by treatment with Pb 2+ ions. Study of chlorophyll fluorescence in suspension of spinach chloroplasts in the presence of Pb 2+ ions confirmed their site of action in PS2. Using fluorescence spectroscopy, also formation of complexes between Pb 2+ and amino acid residues in photosynthetic proteins was confirmed and constants of complex formation among Pb 2+ and aromatic amino acids were calculated for both studied lead compounds.

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Section: Resultssupporting

confidence: 94%

“…Similar inhibition of DCPIP photoreduction caused by Pb 2+ was also observed by Miles at al. (1972), Rashid and Popovic (1990), Prasad et al (1991) and Romanowska et al (2012 . For Pb(CH 3 COO) 2 , k 1 = 141 and k 2 = 3162 .…”

Section: Resultsmentioning

confidence: 99%

Effect of Pb2+ ions on photosynthetic apparatus

Šeršeň¹,

Kráľová²,

Peško³

et al. 2014

gpb

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“…The movements may also be modified by other environmental factors [ 19 , 106 ]. In particular, lead ions are especially dangerous to the correct functioning of the photosynthetic apparatus [ 107 , 108 , 109 ]. Therefore, the toxicity of the metal in the context of chloroplast functioning in various light conditions should be determined—especially when lead is an additional stressor.…”

Section: Discussionmentioning

confidence: 99%

Pb-Induced Avoidance-Like Chloroplast Movements in Fronds of Lemna trisulca L.

et al. 2015

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Lead ions are particularly dangerous to the photosynthetic apparatus, but little is known about the effects of trace metals, including Pb, on regulation of chloroplast redistribution. In this study a new effect of lead on chloroplast distribution patterns and movements was demonstrated in mesophyll cells of a small-sized aquatic angiosperm Lemna trisulca L. (star duckweed). An analysis of confocal microscopy images of L. trisulca fronds treated with lead (15 μM Pb2+, 24 h) in darkness or in weak white light revealed an enhanced accumulation of chloroplasts in the profile position along the anticlinal cell walls, in comparison to untreated plants. The rearrangement of chloroplasts in their response to lead ions in darkness was similar to the avoidance response of chloroplasts in plants treated with strong white light. Transmission electron microscopy X-ray microanalysis showed that intracellular chloroplast arrangement was independent of the location of Pb deposits, suggesting that lead causes redistribution of chloroplasts, which looks like a light-induced avoidance response, but is not a real avoidance response to the metal. Furthermore, a similar redistribution of chloroplasts in L. trisulca cells in darkness was observed also under the influence of exogenously applied hydrogen peroxide (H2O2). In addition, we detected an enhanced accumulation of endogenous H2O2 after treatment of plants with lead. Interestingly, H2O2-specific scavenger catalase partly abolished the Pb-induced chloroplast response. These results suggest that H2O2 can be involved in the avoidance-like movement of chloroplasts induced by lead. Analysis of photometric measurements revealed also strong inhibition (but not complete) of blue-light-induced chloroplast movements by lead. This inhibition may result from disturbances in the actin cytoskeleton, as we observed fragmentation and disappearance of actin filaments around chloroplasts. Results of this study show that the mechanisms of the toxic effect of lead on chloroplasts can include disturbances in their movement and distribution pattern.

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“…The stress resulting from heavy metals causes poorer efficiency in the PSII reaction, triggering inhibition in the Acta Scientiarum. Agronomy, v. 40, e30392, 2018 phosphorylation reaction (Romanowska, Wasilewska, Fristedt, Vener, & Zienkiewicz, 2012) at the association of thylakoids with polyamine molecules, in turn inducing a process of readaptation in the photosynthetic apparatus at the molecular level (Abreu, Coscione, Pires, & Paz-Ferreiro, 2012). Measuring chlorophyll fluorescence is nondestructive, and it can evaluate changes in chloroplast membranes and elucidate damage in the photosynthetic apparatuses of plants (Krause & Weis, 1991).…”

Section: Chlorophyll Fluorescencementioning

confidence: 99%

<b>Metabolic alterations and X-ray chlorophyll fluorescence for the early detection of lead stress in castor bean (<i>Ricinus communis</i>) plants

Nascimento

Marques

2018

Acta Sci. Agron.

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The remediation of lead-contaminated areas poses a serious challenge to soil chemists because Pb has low solubility in soil. Thus, Pb phytostabilization is considered to be an attractive remediation technique. Castor bean (Ricinus communis) is an oilseed crop known for its tolerance to heavy metals, and our aim was to assess the early detection of Pb toxicity and the effects of Pb on the biomass, photosynthetic pigments, antioxidative enzyme activities, and total soluble proteins of this plant. Specimens were grown in a nutrient solution spiked with Pb concentrations of 25, 50, 100, 150, or 200 µmol L-1. A control without Pb was also grown. The results show that X-ray chlorophyll fluorescence is an efficient technique for the early detection of photosystem II alterations driven by Pb toxicity. Castor bean was tolerant to the Pb doses tested; plants presented no changes in photosynthetic pigments, defense enzyme activities, or total soluble proteins in leaves. Given its ability to tolerate and accumulate Pb in its roots, castor bean is a viable alternative for phytostabilization and phytoattenuation of lead-contaminated areas. It is also economically attractive for industrial and biofuel oil production while being used for remediation.

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Phosphorylation of PSII proteins in maize thylakoids in the presence of Pb ions

Cited by 28 publications

References 58 publications

Effect of Pb2+ ions on photosynthetic apparatus

Effect of Pb2+ ions on photosynthetic apparatus

Pb-Induced Avoidance-Like Chloroplast Movements in Fronds of Lemna trisulca L.

<b>Metabolic alterations and X-ray chlorophyll fluorescence for the early detection of lead stress in castor bean (<i>Ricinus communis</i>) plants

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