2001
DOI: 10.1016/s0092-8674(01)00362-2
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Phosphorylation of the Cohesin Subunit Scc1 by Polo/Cdc5 Kinase Regulates Sister Chromatid Separation in Yeast

Abstract: At the onset of anaphase, a caspase-related protease (separase) destroys the link between sister chromatids by cleaving the cohesin subunit Scc1. During most of the cell cycle, separase is kept inactive by binding to an inhibitory protein called securin. Separase activation requires proteolysis of securin, which is mediated by an ubiquitin protein ligase called the anaphase-promoting complex. Cells regulate anaphase entry by delaying securin ubiquitination until all chromosomes have attached to the mitotic spi… Show more

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Cited by 334 publications
(325 citation statements)
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“…In contrast to vertebrate cells, which remove chromatinbound cohesin in a two-step fashion, yeast cells remove cohesin in one step through separase-mediated cleavage. During cohesin displacement in S. cerevisiae, the cohesin subunit SCC1 is phosphorylated by the Polo-homolog Cdc5 (Alexandru et al, 2001). This phosphorylation event strongly enhances SCC1-cleavage by separase and is essential for proper chromosome segregation.…”
Section: Plk1 and Condensation/separation Of Chromosomesmentioning
confidence: 99%
“…In contrast to vertebrate cells, which remove chromatinbound cohesin in a two-step fashion, yeast cells remove cohesin in one step through separase-mediated cleavage. During cohesin displacement in S. cerevisiae, the cohesin subunit SCC1 is phosphorylated by the Polo-homolog Cdc5 (Alexandru et al, 2001). This phosphorylation event strongly enhances SCC1-cleavage by separase and is essential for proper chromosome segregation.…”
Section: Plk1 and Condensation/separation Of Chromosomesmentioning
confidence: 99%
“…Also it phosphorylates cohesin Scc1 and facilitates its cleavage by the separase Esp1 (Alexandru et al, 2001). The lethality in cdc5-1 mutants caused by the overexpression of PP2A subunits may result from the negative effects of PP2A on mitotic exit or on other Cdc5 related cell cycle processes.…”
Section: Genetic Screen For Genes Lethal To Cdc5-1 When Overexpressedmentioning
confidence: 99%
“…The many functions of Plk1 at mitosis [87] are well accounted for by the variety of substrates so far identified for this kinase. These consist of proteins involved with chromosome segregation, such as cohesin [88], microtubules dynamics [89] and nucleation [90] as well as cytokinesis [91]. In addition to the regulation of mechanistic aspects of mitosis, Plk1 contributes to set the timing of entry and transition through mitosis by phosphorylating APC/C subunits [92,93].…”
Section: Polo-like Kinasementioning
confidence: 99%