Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

Camp, Roel G.L. op den; Kuhlemeier, Cris

doi:10.1093/nar/26.9.2058

Cited by 22 publications

(13 citation statements)

References 34 publications

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“…A pheromone-induced cell cycle arrest correlates with phosphorylation of eIF4A at the very N-terminal serine of the protein (62). eIF4A phosphorylation also seems to be part of developmental processes in other organisms including pollen tube germination in tobacco (77).…”

Section: Discussionmentioning

confidence: 99%

The Saccharomyces Homolog of Mammalian RACK1, Cpc2/Asc1p, Is Required for FLO11-dependent Adhesive Growth and Dimorphism

Valerius¹,

Kleinschmidt²,

Rachfall

et al. 2007

Molecular & Cellular Proteomics

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Nutrient starvation results in the interaction of Saccharomyces cerevisiae cells with each other and with surfaces. Adhesive growth requires the expression of the FLO11 gene regulated by the Ras/cAMP/cAMP-dependent protein kinase, the Kss1p/MAPK, and the Gcn4p/general amino acid control pathway, respectively. Proteomics two-dimensional DIGE experiments revealed post-transcriptionally regulated proteins in response to amino acid starvation including the ribosomal protein Cpc2p/Asc1p. This putative translational regulator is highly conserved throughout the eukaryotic kingdom and orthologous to mammalian RACK1. Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells. In addition, the diploid Flo11p-dependent pseudohyphal growth during nitrogen limitation was CPC2/ASC1-dependent. A more detailed analysis revealed that a CPC2/ASC1 deletion caused increased sensitivity to cell wall drugs suggesting that the gene is required for general cell wall integrity. Phosphoproteome and Western hybridization data indicate that Cpc2p/ Asc1p affected the phosphorylation of the translational initiation factors eIF2␣ and eIF4A and the ribosome-associated complex RAC.

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Section: Discussionmentioning

confidence: 99%

The Saccharomyces Homolog of Mammalian RACK1, Cpc2/Asc1p, Is Required for FLO11-dependent Adhesive Growth and Dimorphism

Valerius¹,

Kleinschmidt²,

Rachfall

et al. 2007

Molecular & Cellular Proteomics

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“…Eukaryotic genomes contain a multitude of specialised RNA helicases that may show specificity to substrate sequences or structures [19]. Some members of the eIF-4A subfamily, might be specifically involved in pollen tube germination, since there is a pollen specific isoform and phosphorylation of other isoforms occurs during germination [20]. Likewise, this DEAD box RNA helicase could have a specific function in appressoria formation.…”

Section: Discussionmentioning

confidence: 99%

A proteomics study ofin vitro cyst germination and appressoria formation inPhytophthora infestans

2005

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A proteomics study using two-dimensional gel electrophoresis (2-DE) and mass spectrometry was performed on Phytophthora infestans. Proteins from cysts, germinated cysts and appressoria grown in vitro were isolated and separated by 2-DE. Statistical quantitative analysis of the protein spots from five independent experiments of each developmental stage revealed significant up-regulation of ten spots on gels from germinated cysts compared to cysts. Five spots were significantly up-regulated on gels from appressoria compared to germinated cysts and one of these up-regulated spots was not detectable on gels from cysts. In addition, one spot was significantly down-regulated and another spot not detectable on the gels from appressoria. The corresponding proteins to 13 of these spots were identified with high confidence using tandem mass spectrometry and database searches. The functions of the proteins that were up-regulated in germinated cysts and appressoria can be grouped into the following categories: protein synthesis (e.g. a DEAD box RNA helicase), amino acid metabolism, energy metabolism and reactive oxygen species scavenging. The spot not detected in appressoria was identified as the P. infestans crinkling- and necrosis-inducing protein CRN2. The identified proteins are most likely involved in the establishment of the infection of the host plant.

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“…In Xenopus, eIF4A-II seems to be involved in the development of neuroectodermus (Morgan and Sargent, 1997), and in tobacco, eIF4A-8 may play a role in the development of gametophyte (op den Camp and Kuhlemeier, 1998). Moreover, the activity of specific eIF4A-related proteins has been directly related to the growth status of the cell.…”

Section: Ancient Eif4amentioning

confidence: 99%

Functional diversity of the eukaryotic translation initiation factors belonging to eIF4 families

Hernández

Vázquez-Pianzola

2005

Mechanisms of Development

131

105

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Protein synthesis in eukaryotic cells is fundamental for gene expression. This process involves the binding of an mRNA molecule to the small ribosomal subunit in a group of reactions catalyzed by eukaryotic translation initiation factors (eIF) eIF4. To date, the role of each of the four eIF4, i.e. eIF4E, eIF4G, eIF4A and eIF4B, is well established. However, with the advent of genome-wide sequencing projects of various organisms, families of genes for each translation initiation factor have been identified. Intriguingly, recent studies have now established that certain eIF4 proteins can promote or inhibit translation of specific mRNAs, and also that some of them are active in processes other than translation. In addition, there is evidence of tissue- and developmental-stage-specific expression for some of these proteins. These new findings point to an additional level of complexity in the translation initiation process. In this review, we analyze the latest advances concerning the functionality of members of the eIF4 families in eukaryotic organisms and discuss the implications of this in the context of our current understanding of regulation of the translation initiation process.

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Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

Cited by 22 publications

References 34 publications

The Saccharomyces Homolog of Mammalian RACK1, Cpc2/Asc1p, Is Required for FLO11-dependent Adhesive Growth and Dimorphism

The Saccharomyces Homolog of Mammalian RACK1, Cpc2/Asc1p, Is Required for FLO11-dependent Adhesive Growth and Dimorphism

A proteomics study ofin vitro cyst germination and appressoria formation inPhytophthora infestans

Functional diversity of the eukaryotic translation initiation factors belonging to eIF4 families

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