Phosphorylcholine‐bearing components in homogenates of nematodes

Pery, P.; Petit, Agnès; Poulain, J; Luffau, G.

doi:10.1002/eji.1830040914

Cited by 77 publications

(35 citation statements)

References 10 publications

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“…A major post-translational modification of parasitic helminth antigens is apparently PC. This antigenic determinant has been detected in nematodes (5,8,34,35), in trematodes, including Schistosoma mansoni (9), and in the cestode Bothriocephalus scorpii (36). In fact, the frequency of serological cross-reactivity between cestodes, trematodes and, in particular, nematodes (37) may be accounted for by the broad distribution of PCbearing molecules.…”

Section: Discussionmentioning

confidence: 99%

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Lochnit¹,

Dennis²,

Ulmer³

et al. 1998

Journal of Biological Chemistry

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The isolated neutral glycosphingolipid fraction from the pig parasitic nematode, Ascaris suum, was fractionated by silica gel chromatography to yield a neutral and a zwitterionic glycosphingolipid fraction, the latter of which mainly contained two zwitterionic glycosphingolipids termed components A and C. Preliminary chemical characterization with hydrofluoric acid treatment and immunochemical characterization with a phosphocholine-specific monoclonal antibody indicated that both components contained phosphodiester substitutions: phosphocholine for component A, and phosphocholine and phosphoethanolamine for component C. Both components were biologically active in inducing human peripheral blood mononuclear cells to release the inflammatory monokines tumor necrosis factor ␣, interleukin 1, and interleukin 6. Component A was the more bioactive molecule, and its biological activity was abolished on removal of the phosphocholine substituent by hydrofluoric acid. The glycosphingolipid components were structurally analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, liquid secondary ion mass spectrometry, methylation analysis, 1 H NMR spectroscopy, exoglycosidase cleavage, and ceramide analysis. Their chemical structures were elucidated to be (see Structure I below), The carbohydrate moiety oligosaccharide core was characterized as belonging to the arthro series of protostomial glycosphingolipids. The ceramide moiety was distinguished by (R)-2-hydroxytetracosanoic acid as the dominant fatty acid species and by the C17 iso-branched sphingosine and sphinganine bases, 15-methylhexadecasphing-4-enine and 15-methylhexadecasphinganine, respectively.Analyses of the immunoreactivity between neutral fraction glycolipids derived from various species of parasitic nematodes have indicated a high degree of serological cross-reactivity (1, 2). The structural basis for this immunological cross-reactivity between parasitic nematodes at the level of glycolipids is at present unknown. Structural studies on the neutral fraction glycosphingolipids from adults of the porcine parasitic nematode Ascaris suum have revealed that the identified arthro series oligosaccharide chain was not immunogenic, i.e. did not exhibit immunoreactivity toward infection sera from A. suuminfected mice (2, 3). However, a zwitterionic glycosphingolipid fraction was also isolated from A. suum that demonstrated a phosphodiester sidechain as a structural modification of possibly phosphocholine (PC) 1 and phosphoethanolamine (PE). In addition, these zwitterionic glycolipids were immunogenic/antigenic, i.e. exhibited immunoreactivity toward infection sera from A. suum-infected mice (2).PC-containing macromolecules have been regularly detected * This project was supported by the Deutsche Forschungsgemeinschaft (SFB 535 and Graduiertenkolleg Molecular Biology and Pharmacology) and by the Bundesministerium fü r Bildung, Wissenschaft, Forschung und Technologie Grant 01 KI 9471. The costs of publication of this article were defrayed in...

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Section: Discussionmentioning

confidence: 99%

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Lochnit¹,

Dennis²,

Ulmer³

et al. 1998

Journal of Biological Chemistry

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“…PC is a widely distributed hapten that reacts with C-reactive protein -an acute phase protein which appears in serum during certain infections, inflammatory conditions and tissue damage (Anderson & McCarty, 1950). PC-like determinants have been found in bacteria (Brundish & Baddiley, 1968), parasites (Pery et al, 1974) and in fungi such as the dermatophytes E.Jloccosum and T. rnentagrophytes as well as Aspergillus fumigatus (Pepys & Longbottom, 1971). The PC-like component of E. joccosurn and A .…”

Section: Discussionmentioning

confidence: 99%

Production and Immunological Characterization of a Monoclonal Antibody to Trichophyton quinckeanum: Interaction with Phosphorylcholine-bearing Components

Calderón¹,

Shennan²

1987

Microbiology

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A monoclonal antibody (Tq-1) that interacts with the phosphorylcholine (PC)-bearing antigens of Trichophyton quinckeanum was produced by fusion of myeloma cells (JKAg-8) with spleen cells of BALB/c mice immunized with an alum-precipitated fraction of T . quinckeanum cytoplasmic antigen. It was characterized as an IgM class antibody by immunodiffusion using anti-Ig heavy chain specific reagents, ELISA using immunoglobulin-specific peroxidaseconjugated antibodies, and by gel filtration chromatography; it showed high affinity for Staphylococcus aureus protein-A. Interaction of Tq-1 with PC-like antigens of T. quinckeanum was demonstrated by inhibition studies using ELISA, immunoblotting, immunoprecipitation and immuno electron microscopy techniques. The binding activity of Tq-1 antibody with a range of dermatophyte proteins was completely inhibited by prior incubation with PC hapten. Moreover, dermatophyte antigens reacting with the monoclonal antibody reacted strongly with sera from chronically infected mice. Dermatophyte antigens derived from both young (24 h) and old (20 d) cultures reacted with Tq-1 and this binding was inhibited by PC, suggesting that Tq-1 target antigen PC appears at an early stage during fungal growth and remains throughout its life.

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“…In our accompanying paper (Calderon & Shennan, 1987) the results indicated that the hapten PC, a structural component of dermatophytes (Baldo et al, 1977), appeared to be a dominant antigen during dermatophyte infections in mice. The hapten PC, found also in bacteria (Brundish & Baddiley, 1968) and parasites (Pery et al, 1974), binds to the acute phase C-reactive protein and the resulting complex is known to elicit some immunological reactions (Kaplan & Volanakis, 1974).…”

Section: Discussionmentioning

confidence: 99%

Circulating Antigens and Antibodies in Human and Mouse Dermatophytosis: Use of Monoclonal Antibody Reactive to Phosphorylcholine-like Epitopes

Calderón

Hay²,

Shennan³

1987

Microbiology

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Phosphorylcholine‐bearing components in homogenates of nematodes

Cited by 77 publications

References 10 publications

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Structural Elucidation and Monokine-inducing Activity of Two Biologically Active Zwitterionic Glycosphingolipids Derived from the Porcine Parasitic Nematode Ascaris suum

Production and Immunological Characterization of a Monoclonal Antibody to Trichophyton quinckeanum: Interaction with Phosphorylcholine-bearing Components

Circulating Antigens and Antibodies in Human and Mouse Dermatophytosis: Use of Monoclonal Antibody Reactive to Phosphorylcholine-like Epitopes

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