Photo-dynamics of BLUF domain containing adenylyl cyclase NgPAC3 from the amoeboflagellate Naegleria gruberi NEG-M strain

Penzkofer, Alfons; Tanwar, Meenakshi; Veetil, Sindhu Kandoth; Kateriya, Suneel; Stierl, Manuela; Hegemann, Peter

doi:10.1016/j.jphotochem.2014.04.017

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…FEBS Letters 590 (2016) 2146-2157 ª 2016 Federation of European Biochemical Societies photodynamic characteristic of the protein but modulate the photocycle properties of the protein. Such alteration in the photocycle of the BLUF domain could also be applied in other BLUF-coupled complex protein, e.g., PAC [36][37][38][39][40]. These BLUF-coupled adenylyl cyclases (PAC) have been used as the optogenetic tool to manipulate the cAMP level in the cells [37,41,42].…”

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confidence: 99%

Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor

et al. 2016

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Edited by Richard CogdellBLUF domains are flavin-based photoreceptors which receive the blue light signal and are involved in the sensory transduction. We report a short BLUF photoreceptor (SnfB) in Stenotrophomonas sp. We have investigated photodynamic properties of C terminus truncated and several mutated SnfB proteins. Deletion of the extended C-terminal residues alters the thermal recovery kinetics and also affects the integrity of the SnfB protein. Mutagenesis studies demonstrated that the conserved residues within and outside the flavinbinding pocket also regulates the photocycle properties of the protein. These studies suggest that the C-terminal residues outside the BLUF domain can tune the photodynamic properties of the BLUF protein.

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confidence: 99%

Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor

et al. 2016

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“…PACs (NgPAC1, NgPAC2, NgPAC3, and bPAC) exhibit typical BLUF photochemistry with varying recovery kinetics from signaling state to dark state after photoactivation with blue light. It also showed in vitro light-induced cyclase activity with cyclase activities variable in the amplitude 48 , 50 , 65 , 66 . EuPAC and bPAC have been used for modulation of cAMP level in cells 47 , 48 , 67 .…”

Section: Discussionmentioning

confidence: 89%

“…It represents the time required for a photoreceptor to come back from signaling state to ground state. Biophysical studies demonstrated the fast recovery kinetics of bPAC 48 and NgPAC1 65 , while slower recovery kinetics of NgPAC2 66 and NgPAC3 50 have been reported. These PACs with varying recovery kinetics would enable researchers to manipulate the cAMP levels for brief or extended times.…”

Section: Discussionmentioning

confidence: 95%

“…The first photoactivated adenylyl cyclase (PAC) reported was euPAC from Euglena gracilis and consisted of two BLUF followed by cyclase domain to mediate photo avoidance in the organism 64 . Subsequently, PACs described from Beggiotoa 48 and N. gruberi 50 , 65 , 66 are smaller than euPAC and composed of single BLUF and cyclase domains. PACs (NgPAC1, NgPAC2, NgPAC3, and bPAC) exhibit typical BLUF photochemistry with varying recovery kinetics from signaling state to dark state after photoactivation with blue light.…”

Section: Discussionmentioning

confidence: 99%

“…Our approach harnesses the photoactivated adenylyl cyclases from amoeboflagellate protozoa, Naegleria gruberi (NgPACs) and bacteria Beggiatoa (bPAC). The N. gruberi genome has four PACs (NgPAC1, NgPAC2, NgPAC3, and NgPAC4) genes 50 . We employed the PACs to manipulate the cAMP-signaling pathway and analyzed the cAMP-mediated CREB-dependent gene expression in response to blue light.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool

Tanwar

Khera

Haokip

et al. 2017

Sci Rep

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Cyclic nucleotide signaling pathway plays a significant role in various biological processes such as cell growth, transcription, inflammation, in microbial pathogenesis, etc. Modulation of cyclic nucleotide levels by optogenetic tools has overcome certain limitations of studying transduction cascade by pharmacological agents and has allowed several ways to modulate biological processes in a spatiotemporal manner. Here, we have shown the optogenetic modulation of the cyclooxygenase 2 (Cox-2) gene expression and their downstream effector molecule (PGE2) in HEK-293T cells and the development process of Dictyostelium discoideum via modulating the cyclic nucleotide (cAMP) signaling pathway utilizing photoactivated adenylyl cyclases (PACs) as an optogenetic tool. Light-induced activation of PACs in HEK-293T cells increases the cAMP level that leads to activation of cAMP response element-binding protein (CREB) transcription factor and further upregulates downstream Cox-2 gene expression and their downstream effector molecule prostaglandin E2. In D. discoideum, the light-regulated increase in cAMP level affects the starvation-induced developmental process. These PACs could modulate the cAMP levels in a light-dependent manner and have a potential to control gene expression and their downstream effector molecules with varying magnitude. It would enable one to utilize PAC as a tool to decipher cyclic nucleotide mediated signaling pathway regulations and their mechanism.

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Photoactivated Adenylyl Cyclases: Fundamental Properties and Applications

Iseki

Park

2021

Advances in Experimental Medicine and Biology

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Photo-dynamics of BLUF domain containing adenylyl cyclase NgPAC3 from the amoeboflagellate Naegleria gruberi NEG-M strain

Cited by 10 publications

References 33 publications

Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor

Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor

Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool

Photoactivated Adenylyl Cyclases: Fundamental Properties and Applications

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