Photo-patterning of porous hydrogels for tissue engineering

Bryant, Stephanie J.; Cuy, Janet L.; Hauch, Kip D.; Ratner, Buddy D.

doi:10.1016/j.biomaterials.2006.11.033

Cited by 249 publications

(178 citation statements)

References 25 publications

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“…Since pore size has a big effect on tissue regeneration for different tissue types, 4,39,40 the primary goal of the present study was to demonstrate the ability to tune the porosity and pore size of the macroporous OPF hydrogels through varying the porogen content and size, respectively, during fabrication. Indeed, we were able to fabricate macroporous OPF hydrogels by leveraging the sol-gel reversibility of uncrosslinked gelatin at physiological temperature (Fig.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Cell-Laden Macroporous Biodegradable Hydrogels with Tunable Porosities and Pore Sizes

Wang

Lam

et al. 2015

Tissue Engineering Part C: Methods

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In this work, we investigated a cytocompatible particulate leaching method for the fabrication of cell-laden macroporous hydrogels. We used dehydrated and uncrosslinked gelatin microspheres as leachable porogens to create macroporous oligo(poly(ethylene glycol) fumarate) hydrogels. Varying gelatin content and size resulted in a wide range of porosities and pore sizes, respectively. Encapsulated mesenchymal stem cells (MSCs) exhibited high viability immediately following the fabrication process, and culture of cell-laden hydrogels revealed improved cell viability with increasing porosity. Additionally, the osteogenic potential of the encapsulated MSCs was evaluated over 16 days. Overall, this study presents a robust method for the preparation of cell-laden macroporous hydrogels with desired porosity and pore size for tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Fabrication of Cell-Laden Macroporous Biodegradable Hydrogels with Tunable Porosities and Pore Sizes

Wang

Lam

et al. 2015

Tissue Engineering Part C: Methods

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“…Bryant et al 33) reported elongation, spreading and fibrillar formation of C2C12 cells cultured on a poly-HEMA hydrogel with immobilized type I collagen, demonstrating its benefit as a degradable scaffold. It is considered that, by controlling the polymerization rate, resinous materials can be applied diversely for tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

et al. 2010

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This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.

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“…Diffusion studies demonstrated that cell viability and function were improved in cells that were closer to the perfused channels, presumably because of increased nutrient exchange. 95 The combination of controlled pore size throughout a poly(2-hydroxyethyl methacrylate) hydrogel, created with spherical sacrificial elements, with photopatterned microchannels led to improved elongation, spreading, and fibrillar formation of C2C12 myoblasts. 96 Similarly, focused photoablation achieved using pulsed lasers in PEGylated fibrinogen hydrogels successfully created microchannels of controlled size that were shown to drive the directional growth of neurites.…”

mentioning

confidence: 99%

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Annabi

Nichol

Zhong

et al. 2010

Tissue Engineering Part B: Reviews

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Photo-patterning of porous hydrogels for tissue engineering

Cited by 249 publications

References 25 publications

Fabrication of Cell-Laden Macroporous Biodegradable Hydrogels with Tunable Porosities and Pore Sizes

Fabrication of Cell-Laden Macroporous Biodegradable Hydrogels with Tunable Porosities and Pore Sizes

Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

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