Photo-reactive polyvinylalcohol for photo-immobilized microarray

Ito, Yoshihiro; Nogawa, Masayuki; Takeda, Masami; Shibuya, Tohru

doi:10.1016/j.biomaterials.2004.02.019

Cited by 42 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell-substrate interaction is mainly based on the recognition of Arg-Gly-Asp (RGD) sequences by receptors located on the cell surface. As AWP allows easy immobilization of specific proteins, it has been studied for application to microarray chips and cell adhesion assays [37]. It is generally accepted that hydrophilic polymers such as poly(ethylene oxide)-based polymers and phosphorylcholine-functioned polymers do not allow protein adsorption at their surface.…”

Section: Discussionmentioning

confidence: 99%

Surface immobilization of biocompatible phospholipid polymer multilayered hydrogel on titanium alloy

Choi

Konno

Matsuno

et al. 2008

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Surface immobilization of biocompatible phospholipid polymer multilayered hydrogel on titanium alloy

Choi

Konno

Matsuno

et al. 2008

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…36 An AWP solution was spin coated on a glass slide, and then pattern-immobilized in the presence of a photomask. When the adsorption onto the glass slide was measured, the pattern formed was stable in organic solvents including methanol, acetone, and tetrahydrofuran.…”

Section: Proteinsmentioning

confidence: 99%

Photoreactive Polymers For Microarray Chips

Zhou

Sivakumar

Son

et al. 2014

Photocured Materials

Self Cite

View full text Add to dashboard Cite

A photoimmobilization technology using photoreactive and nonbiofouling polymers was developed for the preparation of microarray biochips. This photoimmobilization enabled the convenient covalent immobilization of various types of biological components. In addition, by using nonbiofouling polymers as matrixes it was possible to reduce the nonspecific interactions with the biological components. Various proteins, allergens, autoantigens, viruses, and cells were microarrayed using this technique. This microarray technology will be useful not only for fundamental investigations of biological interactions, but also for applications in clinical analysis.

show abstract

“…However, covalent attachment generally requires special functional groups, such as amino or carboxyl groups. Hence, immobilization by photo-irradiation was found to be a suitable alternative and has been used in the preparation of microarray formats by our group and other researchers [11]–[19].…”

Section: Introductionmentioning

confidence: 96%

Novel Microarrays for Simultaneous Serodiagnosis of Multiple Antiviral Antibodies

et al. 2013

Self Cite

View full text Add to dashboard Cite

We developed an automated diagnostic system for the detection of virus-specific immunoglobulin Gs (IgGs) that was based on a microarray platform. We compared efficacies of our automated system with conventional enzyme immunoassays (EIAs). Viruses were immobilized to microarrays using a radical cross-linking reaction that was induced by photo-irradiation. A new photoreactive polymer containing perfluorophenyl azide (PFPA) and poly(ethylene glycol) methacrylate was prepared and coated on plates. Inactivated measles, rubella, mumps, Varicella-Zoster and recombinant Epstein-Barr viruse antigen were added to coated plates, and irradiated with ultraviolet light to facilitate immobilization. Virus-specific IgGs in healthy human sera were assayed using these prepared microarrays and the results obtained compared with those from conventional EIAs. We observed high correlation (0.79–0.96) in the results between the automated microarray technique and EIAs. The microarray-based assay was more rapid, involved less reagents and sample, and was easier to conduct compared with conventional EIA techniques. The automated microarray system was further improved by introducing reagent storage reservoirs inside the chamber, thereby conserving the use of expensive reagents and antibodies. We considered the microarray format to be suitable for rapid and multiple serological diagnoses of viral diseases that could be developed further for clinical applications.

show abstract

Photo-reactive polyvinylalcohol for photo-immobilized microarray

Cited by 42 publications

References 27 publications

Surface immobilization of biocompatible phospholipid polymer multilayered hydrogel on titanium alloy

Surface immobilization of biocompatible phospholipid polymer multilayered hydrogel on titanium alloy

Photoreactive Polymers For Microarray Chips

Novel Microarrays for Simultaneous Serodiagnosis of Multiple Antiviral Antibodies

Contact Info

Product

Resources

About