Photoacoustic and fluorescent effects in multilayer plasmon‐dye interfaces

Новоселова, М. В.; Bratashov, Daniil N.; Sarimollaoglu, Mustafa; Nedosekin, Dmitry A.; Harrington, Walter N.; Watts, Alex; Han, Mi‐Kyung; Khlebtsov, Boris N.; Galanzha, Ekaterina I.; Gorin, Dmitry A.; Zharov, Vladimir P.

doi:10.1002/jbio.201800265

Cited by 16 publications

(11 citation statements)

References 60 publications

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“…The chromophore role can play inorganic nanoparticles like gold, magnetite nanoparticles, or fluorescent dyes like indocyanine green. [262,264,266,305] It is necessary to remark that indocyanine green is already FDA improved for clinical applications. [365] The presence of magnetite nanoparticle in the shell provides an opportunity to realize the MRI contrast in both T1 and T2 modes, as well as navigation mode obtained by the magnetic field gradient.…”

Section: Discussionmentioning

confidence: 99%

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

et al. 2021

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Wireless nano‐/micromotors powered by chemical reactions and/or external fields generate motive forces, perform tasks, and significantly extend short‐range dynamic responses of passive biomedical microcarriers. However, before micromotors can be translated into clinical use, several major problems, including the biocompatibility of materials, the toxicity of chemical fuels, and deep tissue imaging methods, must be solved. Nanomaterials with enzyme‐like characteristics (e.g., catalase, oxidase, peroxidase, superoxide dismutase), that is, nanozymes, can significantly expand the scope of micromotors’ chemical fuels. A convergence of nanozymes, micromotors, and microfluidics can lead to a paradigm shift in the fabrication of multifunctional micromotors in reasonable quantities, encapsulation of desired subsystems, and engineering of FDA‐approved core–shell structures with tuneable biological, physical, chemical, and mechanical properties. Microfluidic methods are used to prepare stable bubbles/microbubbles and capsules integrating ultrasound, optoacoustic, fluorescent, and magnetic resonance imaging modalities. The aim here is to discuss an interdisciplinary approach of three independent emerging topics: micromotors, nanozymes, and microfluidics to creatively: 1) embrace new ideas, 2) think across boundaries, and 3) solve problems whose solutions are beyond the scope of a single discipline toward the development of micro‐bio‐chemo‐mechanical‐systems for diverse bioapplications.

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Section: Discussionmentioning

confidence: 99%

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

et al. 2021

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“…Based on the safety of PA technology, and the successful use of PAFC in our clinical trials to detect B-CTCs in melanoma patients with anunprecedented 1000-fold improvement in sensitivity [ 17 ], we are positioned to rapidly translate the knowledge gained from our studies into clinical practice, initially by using label-free PAFC detection of melanoma L-CTCs integrated with PA lymphography. The translation of PAFC to other tumors that absorb low levels of light can be achieved by molecular labeling of L-CTCs using low-toxicity, highly absorbing contrast agents such as gold nanoparticles, spasers, layered composite structures (core-shells) and biocompatible natural magnetic nanoparticles [ 10 , 12 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. For molecular labeling, contrast agents should be conjugated with molecules (e.g., antibodies, nanobodies and proteins) specific to CTCs.…”

Section: Discussionmentioning

confidence: 99%

“…PAFC does this by using high pulse-repetition-rate lasers (up to 0.5 MHz) and a focused ultrasound transducer which are insensitive to light scattering and autofluorescence background [ 10 , 11 , 12 , 17 , 18 ]. As a result, PAFC identifies single cells with endogenous (e.g., melanin in melanoma CTCs) or exogenous (e.g., low toxic gold nanoparticles, spasers, and layered composite structures) PA contrast agents in multicellular flow without skin incision and extraction of blood samples (i.e., noninvasively) [ 10 , 11 , 12 , 18 , 19 , 20 , 21 , 22 , 23 ]. Furthermore, the high sensitivity of PAFC requires only a small amount of absorbing contrasts inside a cell to make them detectable [ 18 , 19 , 20 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Lymphatic Circulating Tumor Cells and Progression of Metastatic Disease

Han

Watts

Jamshidi‐Parsian

et al. 2020

Cancers

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The dissemination of circulating tumor cells (CTCs) by lymph fluid is one of the key events in the development of tumor metastasis. However, little progress has been made in studying lymphatic CTCs (L-CTCs). Here, we demonstrate the detection of L-CTCs in preclinical mouse models of melanoma and breast cancer using in vivo high-sensitivity photoacoustic and fluorescent flow cytometry. We discovered that L-CTCs are be detected in pre-metastatic disease stage. The smallest primary tumor that shed L-CTCs was measured as 0.094mm×0.094mm, its volume was calculated as 0.0004 mm3; and its productivity was estimated as 1 L-CTC per 30 minutes. As the disease progressed, primary tumors continued releasing L-CTCs with certain individual dynamics. The integrated assessment of lymph and blood underlined the parallel dissemination of CTCs at all disease stages. However, the analysis of links between L-CTC counts, blood CTC (B-CTC) counts, primary tumor size and metastasis did not reveal statistically significant correlations, likely due to L-CTC heterogeneity. Altogether, our results showed the feasibility of our diagnostic platform using photoacoustic flow cytometry for preclinical L-CTC research with translational potential. Our findings also demonstrated new insights into lymphatic system involvement in CTC dissemination. They help to lay the scientific foundation for the consideration of L-CTCs as prognostic markers of metastasis and to emphasize the integrative assessment of lymph and blood.

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“…Despite the development of many advanced spasers, most of the research in this area still focuses on exploring new schematics 2–9 rather than new applications 4,5 . Recently, we demonstrated the first biological applications of spasers with a fixed wavelength (i.e., with one color) that consisted of plasmonic nanoparticle cores as the optical resonator, with a silica or polymer layer doped with a low-toxicity active-gain medium such as uranine and indocyanine green (ICG), which are both approved for use in humans 5,7 . Here, we introduce photoswitchable spasers that can further extend the applications of spasers, with a focus on their multicolor capacity.…”

Section: Discussionmentioning

confidence: 99%

Photoswitchable Spasers with a Plasmonic Core and Photoswitchable Fluorescent Proteins

Harrington

Новоселова

Bratashov

et al. 2019

Sci Rep

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Photoswitchable fluorescent proteins (PFPs) that can change fluorescence color upon excitation have revolutionized many applications of light such as tracking protein movement, super-resolution imaging, identification of circulating cells, and optical data storage. Nevertheless, the relatively weak fluorescence of PFPs limits their applications in biomedical imaging due to strong tissue autofluorecence background. Conversely, plasmonic nanolasers, also called spasers, have demonstrated potential to generate super-bright stimulated emissions even inside single cells. Nevertheless, the development of photoswitchable spasers that can shift their stimulated emission color in response to light is challenging. Here, we introduce the novel concept of spasers using a PFP layer as the active medium surrounding a plasmonic core. The proof of principle was demonstrated by synthesizing a multilayer nanostructure on the surface of a spherical gold core, with a non-absorbing thin polymer shell and the PFP Dendra2 dispersed in the matrix of a biodegradable polymer. We have demonstrated photoswitching of spontaneous and stimulated emission in these spasers below and above the spasing threshold, respectively, at different spectral ranges. The plasmonic core of the spasers serves also as a photothermal (and potentially photoacoustic) contrast agent, allowing for photothermal imaging of the spasers. These results suggest that multimodal photoswitchable spasers could extend the traditional applications of spasers and PFPs in laser spectroscopy, multicolor cytometry, and theranostics with the potential to track, identify, and kill abnormal cells in circulation.

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Photoacoustic and fluorescent effects in multilayer plasmon‐dye interfaces

Cited by 16 publications

References 60 publications

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

In Vivo Lymphatic Circulating Tumor Cells and Progression of Metastatic Disease

Photoswitchable Spasers with a Plasmonic Core and Photoswitchable Fluorescent Proteins

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