Photoacoustic ocular imaging

Zerda, Adam de la; Paulus, Yannis M.; Teed, Robert; Bodapati, Sunil; Dollberg, Yosh; Khuri‐Yakub, Butrus T.; Blumenkranz, Mark S.; Moshfeghi, Darius M.; Gambhir, Sanjiv S.

doi:10.1364/ol.35.000270

Cited by 132 publications

(111 citation statements)

References 11 publications

Supporting

Mentioning

111

Contrasting

Order By: Relevance

“…The retina of the eye absorbs some of the delivered laser energy, generates heat, undergoes transient thermoelastic expansion, and produces ultrasonic signal. An ultrasound transducer is focused on the retinal surface and detects ultrasonic signals, which are then imaged for both anatomic PAI and functional analysis [16][17][18].…”

Section: Principle Of Paimentioning

confidence: 99%

See 1 more Smart Citation

Novel Retinal Imaging Technologies

Y¹,

Ym²

2017

IJOES

View full text Add to dashboard Cite

Section: Principle Of Paimentioning

confidence: 99%

“…Our group performed PAI of an enucleated pig eye and the eye of living rabbits. We acquired 3D images of the posterior pole (retina, choroid, optic nerve) of the rabbit eye by ultrasound and photoacoustic imaging [18][19][20]. Silverman et al, [21] imaged fresh ex vivo pig eyes by PAI and US.…”

Section: Applications Of Paimentioning

confidence: 99%

Novel Retinal Imaging Technologies

Y¹,

Ym²

2017

IJOES

View full text Add to dashboard Cite

“…[15][16][17][18][19][20] In this letter, we developed a near-real-time virtual intraoperative surgical photoacoustic microscope (VISPAM) by integrating PAM and conventional surgical microscopy. The VISPAM system could obtain, process, and display both PAM and microscopic images at the same time.…”

Section: -5mentioning

confidence: 99%

“…[12][13][14] Because PAM can provide label-free optical absorption information in a non-invasive manner with high contrast and resolution, this modality has widely been used to image tumor angiogenesis, tumor metabolism, brain function, ocular structures, molecular information, etc. [15][16][17][18][19][20] In this letter, we developed a near-real-time virtual intraoperative surgical photoacoustic microscope (VISPAM) by integrating PAM and conventional surgical microscopy. The VISPAM system could obtain, process, and display both PAM and microscopic images at the same time.…”

mentioning

confidence: 99%

In vivo virtual intraoperative surgical photoacoustic microscopy

Han

Lee

Kim

et al. 2013

Applied Physics Letters

View full text Add to dashboard Cite

We developed a virtual intraoperative surgical photoacoustic microscopy system by combining with a commercial surgical microscope and photoacoustic microscope (PAM). By sharing the common optical path in the microscope and PAM system, we could acquire the PAM and microscope images simultaneously. Moreover, by employing a beam projector to back-project 2D PAM images onto the microscope view plane as augmented reality, the conventional microscopic and 2D cross-sectional PAM images are concurrently mapped on the plane via an ocular lens of the microscope in real-time. Further, we guided needle insertion into phantom ex vivo and mice skins in vivo. A surgical microscope is a vital tool during operations since it started being used in otolaryngology.1,2 In spite of the continuous improvement of the surgical microscopy performance, it could mainly provide the enlarged surface images without the sub-surface information. Because of this limitation, surgeons are required to have significant experiences and heavy trainings in current clinical practices. Thus, noninvasive visualization of the sub-surface information should play an important role during ophthalmic, microvascular, and neuro-surgeries. 3-5Conventional optical microscopic techniques such as optical coherence tomography (OCT) and fluorescence microscopy (FM) has been applied for this purpose.6-10 OCT mainly provide microstructures of tissues based on optical scattering while the penetration depth of FM is significantly shallow and requires to use the exogenous contrast agents. However, real-time noninvasive mapping of microvasculatures are crucial to verify bleeding regions, guide vascular reconnection, or delineate angiogenesis during the surgeries. 11 To meet this purpose, we are convinced to develop a noninvasive surgical microscopy system to image microvasculatures and surgical intervention without any contrast agent beyond the skin surface in real-time.Photoacoustic microscopy (PAM) is a rising imaging technique that combines optical excitation and ultrasound detection.12-14 Because PAM can provide label-free optical absorption information in a non-invasive manner with high contrast and resolution, this modality has widely been used to image tumor angiogenesis, tumor metabolism, brain function, ocular structures, molecular information, etc. [15][16][17][18][19][20] In this letter, we developed a near-real-time virtual intraoperative surgical photoacoustic microscope (VISPAM) by integrating PAM and conventional surgical microscopy. The VISPAM system could obtain, process, and display both PAM and microscopic images at the same time. The Hilberttransformed cross-sectional PAM B-scan images were projected back onto the microscope view plane as augmented reality through a home-made optical beam splitter, and then the PAM images were shown via the ocular lens mounted on the microscope. Thus, an additional tabletop display is unnecessary, so it is significantly convenient because surgeons potentially do not need to move their sights during the surgeries. We demo...

show abstract

“…Following our development of numerous imaging agents for specifically labeling target proteins of interest in living mice, [3][4][5][6] we recently designed a new OCT-based imaging system for the retina by developing nanoparticles based on gold nanorods (GNRs), which produce a strong OCT signal. GNRs have been recently used to enhance OCT signals in tissue mimicking phantoms, [7][8][9][10][11] excised porcine eyes, 8 vitreous of mice eyes, 12 anterior chambers and cornea of mice eyes, 13 and in mouse mammary tumors.…”

Section: Introductionmentioning

confidence: 99%

High-resolution contrast-enhanced optical coherence tomography in mice retinae

et al. 2016

Self Cite

View full text Add to dashboard Cite

Abstract. Optical coherence tomography (OCT) is a noninvasive interferometric imaging modality providing anatomical information at depths of millimeters and a resolution of micrometers. Conventional OCT images limit our knowledge to anatomical structures alone, without any contrast enhancement. Therefore, here we have, for the first time, optimized an OCT-based contrast-enhanced imaging system for imaging single cells and blood vessels in vivo inside the living mouse retina at subnanomolar sensitivity. We used bioconjugated gold nanorods (GNRs) as exogenous OCT contrast agents. Specifically, we used anti-mouse CD45 coated GNRs to label mouse leukocytes and mPEG-coated GNRs to determine sensitivity of GNR detection in vivo inside mice retinae. We corroborated OCT observations with hyperspectral dark-field microscopy of formalin-fixed histological sections. Our results show that mouse leukocytes that otherwise do not produce OCT contrast can be labeled with GNRs leading to significant OCT intensity equivalent to a 0.5 nM GNR solution. Furthermore, GNRs injected intravenously can be detected inside retinal blood vessels at a sensitivity of ∼0.5 nM, and GNR-labeled cells injected intravenously can be detected inside retinal capillaries by enhanced OCT contrast. We envision the unprecedented resolution and sensitivity of functionalized GNRs coupled with OCT to be adopted for longitudinal studies of retinal disorders.

show abstract

Photoacoustic ocular imaging

Cited by 132 publications

References 11 publications

Novel Retinal Imaging Technologies

Novel Retinal Imaging Technologies

In vivo virtual intraoperative surgical photoacoustic microscopy

High-resolution contrast-enhanced optical coherence tomography in mice retinae

Contact Info

Product

Resources

About