Photoacoustic spectroscopy (PAS) and its application in photosynthesis research

Buschmann, Claus; Prehn, H.; Lichtenthaler, H. K.

doi:10.1007/bf00018373

Cited by 52 publications

(18 citation statements)

References 44 publications

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“…This technique is described in detail elsewhere (5,6). In brief, thermal photoacoustic signals are used to quantify the conversion of absorbed light to heat in a sample.…”

Section: Photoacoustic Energy Storage Measurementsmentioning

confidence: 99%

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Herbert¹,

Fork²,

Malkin³

1990

Plant Physiol.

119

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Energy storage by cyclic electron flow through photosystem I (PSI) was measured in vivo using the photoacoustic technique. A wide variety of photosynthetic organisms were considered and all showed measurable energy storage by PSI-cyclic electron flow except for higher plants using the C-3 carbon fixation pathway. The capacity for energy storage by PSI-cyclic electron flow alone was found to be small in comparison to that of linear and cyclic electron flows combined but may be significant, nonetheless, under conditions when photosystem 11 is damaged, particularly in cyanobacteria. Light-induced dynamics of energy storage by PSI-cyclic electron flow were evident, demonstrating regulation under changing environmental conditions.The oxygen-evolving photosynthetic apparatus contains two reaction center complexes, designated PSI electron flow through PSI in C3 plants has been proposed to generate ATP over and above that produced by linear electron flow, adjusting the ratio of ATP to NADPH generated by the light reactions of photosynthesis in accordance with the needs of the plant (1). As an example, cyclic electron flow through PSI has been proposed as a source of ATP for repair of PSII units damaged by environmental stress, since PSI is typically much less susceptible to stress than PSII (8, 9). Much of the ambiguity concerning the function and significance of cyclic electron flow through PSI is due to the difficulty of measuring it in whole cells and tissues. Most previous studies ofPSI-cyclic electron flow and accompanying phosphorylation have necessarily used either in vitro measurements of thylakoid fragments or somewhat ambiguous light-induced absorbance changes in whole cells or tissues (12,17). The photoacoustic method for measurement of photosynthetic energy storage is well suited for study of PSI-cyclic electron flow, however, because it is capable of simple, direct, and quantitative measurement of energy storage by cyclic electron flow in intact leaves and algae as well as in thylakoid preparations (9,10,16,18). Presumably, the bulk of such energy storage by PSI-cyclic electron flow represents photophosphorylation of ADP.In the present report, the occurrence, capacity, and regulation of energy storage by PSI-cyclic electron flow in whole tissues of a variety of photosynthetic organisms are characterized using the photoacoustic method. MATERIALS AND METHODS Plant Material

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“…This technique is described in detail elsewhere (5,6). In brief, thermal photoacoustic signals are used to quantify the conversion of absorbed light to heat in a sample.…”

Section: Photoacoustic Energy Storage Measurementsmentioning

confidence: 99%

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Herbert¹,

Fork²,

Malkin³

1990

Plant Physiol.

119

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“…The energy of heat emission of the photosynthetic pigments is lost for photosynthesis just as the energy of the chlorophyll fluorescence (Buschmann et al 1984, Butler 1978.…”

Section: Introductionmentioning

confidence: 99%

Induction kinetics of heat emission before and after photoinhibition in cotyledons of Raphanus sativus

Buschmann

1987

Photosynth Res

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Heat emitted during non-radiative de-excitation was determined in vivo by the photoacoustic method. The dependence of the photoacoustic signal on the length of the pulses (modulation frequency) of the excitation light and the effect of continuous light, which saturates photosynthesis but does not directly contribute to the signal, are described. The induction kinetic of heat emission measured with intact leaves differed only slightly from the induction kinetic of fluorescence (Kautsky effect) detected in parallel. The photoacoustic signal at high modulation frequencies (279 Hz), which represents the signal of heat emission, and the photoacoustic signal at low modulation frequencies (17 Hz), interpreted as a signal of pulsed oxygen evolution superimposed on the heat emission, were measured with leaves before and after photoinhibition. It was demonstrated that after photoinhibition the decrease in fluorescence yield and in photosynthetic activity (here detected as photoacoustic signal at 17 Hz) are paralleled by an increase in the yield of non-radiative deexcitation (photoacoustic signal at 279 Hz). The increase of heat emission, which has been hypothized for photoinhibited leaves, could now be proved by measuring the induction kinetics of the photoacoustic signal.

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“…This technique has been successfully applied to study the energy storage and relative ' This work has been financially supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and by Entente Auxiliaire Canada-Quebec sur le Developpement Agro-alimnentaire. quantum yield of photosynthetic oxygen evolution of an intact leaf (4,8,10,19), and a close correlation between photoacoustic and fluorescence or conventional gas exchange measurements has been presented (8,29).…”

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confidence: 85%

SO₂ Effect on Photosynthetic Activities of Intact Sugar Maple Leaves as Detected by Photoacoustic Spectroscopy

Veeranjaneyulu¹,

N’soukpoé‐Kossi²,

Leblanc³

1991

Plant Physiol.

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Short-term (4 hours) effect of different concentrations of SO2 fumigation on in vivo photochemical activities of sugar maple (Acer saccharum Marsh.) leaves was investigated using photoacoustic spectroscopy. The relative quantum yield of 02 evolution (ratio of 02 signal to the photothermal signal) and photochemical energy storage are increased by 0.05 microliter per liter of SO2. This increase is more pronounced in 5 to 7 year old saplings than in 3 month old seedlings. Both oxygen-relative quantum yield and energy storage of seedlings are inhibited by increased concentrations of SO2 and the inhibition is concentration dependent. The inhibition is greater in seedlings than in saplings at 2 microliters per liter of SO2, indicating the more susceptible nature of seedlings. The present study indicates a concentration dependent differential effect of SO2 on photochemical activities of sugar maple leaves.Sulfur dioxide is a widespread gaseous pollutant affecting plant growth and yield (6). It reduces net photosynthetic CO2 assimilation by affecting stomatal and nonstomatal components of photosynthesis (6,12,22,23,27). Several investigators reported that SO2 inhibits photosynthetic electron transport and inactivates Calvin-cycle enzymes in chloroplasts isolated from SO2 fumigated or sulfite-treated leaves (2,22,23,27). However, there are also reports indicating increased rates ofelectron transport, NADP reduction, and CO2 fixation in sulfite-treated chloroplasts or chloroplasts isolated from SO,-fumigated leaves (13,15,30). Recently, it has been demonstrated that sulfite shows an artificial inhibitory effect on photosynthetic electron transport by interacting with oxygen and certain electron acceptors in the assay medium (3). So, the observed inhibition of photosynthetic electron transport in the in vitro studies may be overestimated.Limited information is available on the effect of different concentrations of SO, on in vivo photochemical activities of an intact leaf. Hence, in the present study, we examined the short term SO2 fumigation effect on the photochemical activities of sugar maple leaves using a relatively recent technique of photoacoustic spectroscopy. This technique has been successfully applied to study the energy storage and relative '

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Photoacoustic spectroscopy (PAS) and its application in photosynthesis research

Cited by 52 publications

References 44 publications

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Induction kinetics of heat emission before and after photoinhibition in cotyledons of Raphanus sativus

SO₂ Effect on Photosynthetic Activities of Intact Sugar Maple Leaves as Detected by Photoacoustic Spectroscopy

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Photoacoustic spectroscopy (PAS) and its application in photosynthesis research

Cited by 52 publications

References 44 publications

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Photoacoustic Measurements in Vivo of Energy Storage by Cyclic Electron Flow in Algae and Higher Plants

Induction kinetics of heat emission before and after photoinhibition in cotyledons of Raphanus sativus

SO2 Effect on Photosynthetic Activities of Intact Sugar Maple Leaves as Detected by Photoacoustic Spectroscopy

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SO₂ Effect on Photosynthetic Activities of Intact Sugar Maple Leaves as Detected by Photoacoustic Spectroscopy