Photoactive extracts from Thevetia peruviana with antifungal properties against Cladosporium cucumerinum

Gata-Gonçalves, Lı́gia; Nogueira, J.M.F.; Matos, Olı́via; Sousa, R. Bruno de

doi:10.1016/s1011-1344(03)00024-1

Cited by 51 publications

(28 citation statements)

References 7 publications

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“…Recently, some compounds extracted from several medicinal plants were investigated for their antifungal activity against C. cucumerinum . However, these compounds' activity were not quantitative in the literature 20–22. The data obtained indicated that all kinds of CS salts in this study could effectively inhibit the growth of C. cucumerinum .…”

Section: Resultsmentioning

confidence: 62%

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Antifungal properties of chitosan salts in laboratory media

Ren

Liu

et al. 2011

J of Applied Polymer Sci

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Some aliphatic carboxylic acids were used to produce chitosan (CS) salts by reaction with CS, and their antifungal activity against three kinds of phytopathogens was estimated by hypha measurement in vitro. The fungicidal assessment showed that all of the CS salts had excellent activity against the tested fungi. Their inhibitory indices were 41. 15-64.15, 56.25-76.56, and 35.94-68.75% for Cladosporium cucumerinum (Ell.) et Arthur, Monilinia fructicola (Wint.) Honey, and Fusarium oxysporum sp. Cucumis sativus L., respectively, at 1000 lg/mL; these indices were higher than that of CS. It was confirmed that the amino groups' protonation was important for the antifungal activity of CS derivatives. The substituted groups with stronger electronegativity drew more electrons from the nitrogen atoms in the derivative molecules, which relatively strengthened the polycationic character of the CS derivatives. Therefore, the antifungal activity of the CS salts was improved.

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Section: Resultsmentioning

confidence: 62%

“…However, these compounds' activity were not quantitative in the literature. [20][21][22] The data obtained indicated that all kinds of CS salts in this study could effectively inhibit the growth of C. cucumerinum.…”

Section: Characterization Of the Cs Saltsmentioning

confidence: 99%

Antifungal properties of chitosan salts in laboratory media

Ren

Liu

et al. 2011

J of Applied Polymer Sci

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“…Despite its toxicity, this plant has been reported to promote various biological activities. For example, terpenes isolated from the seed extract exhibited antifungal properties against Cladosporium cucumerinum (25), and flavonols isolated from the leaf extract promoted human immunodeficiency virus type 1 (HIV-1) reverse transcriptase and HIV-1 integrase inhibitory activities (26). Furthermore, a previous study found that thevefolin cardenolide glycoside isolated from the bark extract of T. peruviana showed a reversal effect on TRAIL-resistance in human gastric adenocarcinoma cell lines through the enhancement of mRNA expression of death receptors, DR4 and DR5 (27).…”

Section: Ethanolic Extract Of Thevetia Peruviana Flowers Enhances Tnfmentioning

confidence: 99%

Ethanolic extract of Thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways

2017

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Abstract. Tumor necrosis factor-α (TNF-α) and TNF-related apoptosis-inducing ligand (TRAIL) are promising candidates for cancer treatment due to their ability to induce apoptosis through death receptor stimulation. However, their usage may be limited due to the resistance of cancer cells to TNF-α-and TRAIL-induced apoptosis. Currently, there is interest in screening for natural products that can sensitize cancer cells to TNF-α-and TRAIL-induced apoptosis for their use in combination with TNF-α or TRAIL. It was previously reported that the bark extract of Thevetia peruviana showed a reversal effect on TRAIL-resistance in human gastric adenocarcinoma cell lines. In the present study, the effects of the ethanolic extract of T. peruviana flowers on TNF-α-and TRAIL-induced apoptosis of human cervical cancer HeLa cells were investigated in vitro by determining cell viability and apoptosis using a WST-1 cell proliferation assay and immunoblot analysis, respectively. The ethanolic extract of T. peruviana flowers promoted TNF-α and TRAIL-mediated cell death through the activation of the caspase cascade, poly(ADP-ribose) polymerase and BH3-interacting domain death agonist cleavage. Combined treatment using the extract plus TNF-α resulted in downregulation of anti-apoptotic protein, including myeloid cell leukemia sequence-1, B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis protein and survivin, while the combined treatment with TRAIL downregulated Bcl-XL. Thus, the ethanolic extract of T. peruviana flowers has potential in sensitizing the TNF-α-and TRAIL-induced apoptosis of HeLa cells via the intrinsic and extrinsic pathways. IntroductionChemotherapy is one of the major standard treatments that is beneficial for numerous cancer types due to its efficient induction of tumor cell death by apoptosis. At present, the cancer resistance of chemotherapeutic drugs is a major drawback that leads to the failure of cancer treatments. Chemotherapeutic drugs eliminate tumor cells by the induction of apoptosis via two distinct pathways, which act through the mitochondria (intrinsic signaling pathway) or through death receptors on the cell surface (extrinsic signaling pathway) (1). Induction of apoptosis via the intrinsic signaling pathway is initiated by severe cell stress, including DNA damage, mitotic catastrophe, hypoxia and cell cycle checkpoint defects. The majority of conventional chemotherapeutic drugs trigger DNA damage and induce apoptosis via the intrinsic signaling pathway by the activation of tumor suppressor protein, p53, which leads to the breakdown of mitochondrial membrane and the release of cytochrome c into the cytosol. Cytochrome c binds to the adaptor apoptotic protease activating factor 1, forming an apoptosome. The apoptosome subsequently activates caspase-9 and caspase-3, which results in the cleavage of death substrates (2-4). Defects in intrinsic apoptosis signal transduction, such as the mutation of p53, contributes to the chemotherapeutic drug resistance of cancer (5,6). The extrinsic pa...

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“…The entire plant contains milky juice which is poisonous. The leaves, fruits, seeds and roots are considered as potential source of biologically active compounds 4 , such as insecticides, fungicides and bactericides [4][5][6][7][8][9][10] . The root paste is used against tumours.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Root Extract of Thevetia Peruviana (Pers.) Schum

Bhoyar¹,

Biradar²

2014

Int. Res. J. Pharm.

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The present investigation has been evaluated to find out the antibacterial activity and phytochemical screening of the root extract of Thevetia peruviana (Pers.) K Schum. The antibacterial efficacy of root extracts of Thevetia peruviana was performed in different solvents like Petroleum ether, Ethanol, Methanol, Acetone and Distilled water by using the Soxhlet apparatus. The antibacterial activity was observed against test organisms like Escherichia coli, Salmonella typhi, Klebsiella pneumonae, Pseudomonas aeruginosa, Staphylococcus aureus and Shigella flexinari. The results show that the inhibitory action of petroleum ether extract was relatively higher than that of other extracts against Salmonella typhi. The Methanol and Acetone extract also shows marked inhibition activity against all test organisms. The Amoxicillin was used as a standard. The phytochemical screening reveals the presence of Alkaloids, Flavonoids, Saponins, Tannins, Carbohydrates and Terpenoids.

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Photoactive extracts from Thevetia peruviana with antifungal properties against Cladosporium cucumerinum

Cited by 51 publications

References 7 publications

Antifungal properties of chitosan salts in laboratory media

Antifungal properties of chitosan salts in laboratory media

Ethanolic extract of Thevetia peruviana flowers enhances TNF-α and TRAIL-induced apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways

Antibacterial Activity of Root Extract of Thevetia Peruviana (Pers.) Schum

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