Photoautotrophic growth in suspension culture of cells from the moss, <i>Barbula unguiculata</i>

Takami, Shinji; Nishikawa, S.; Takio, Susumu; Hino, Seiichi

doi:10.1111/j.1399-3054.1988.tb00590.x

Cited by 10 publications

(10 citation statements)

References 17 publications

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“…In contrast to vascular plants, where autotrophy is usually only achieved with elevated levels of CO 2 (Kozai 1991) and increased light levels, many bryophyte species can grow autotrophically (Takami et al 1988;Hohe et al 2002a;Rowntree 2006). Therefore, addition of sucrose to bryophyte cultures is not strictly necessary.…”

Section: In Vitro Culture Of Bryophytesmentioning

confidence: 90%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The use of in vitro techniques for conservation has been rising steadily since their inclusion in The Convention on Biological Diversity and The Global Strategy for Plant Conservation. Unfortunately, bryophytes are often overlooked in conservation initiatives, but they are important in a number of large-scale ecosystem processes, i.e. nutrient, water and carbon cycling. There is a long history of the use of tissue culture in cultivating bryophytes, and many species respond well to in vitro techniques. For 6 yr (2000)(2001)(2002)(2003)(2004)(2005)(2006), The Royal Botanic Gardens, Kew and the UK statutory conservation agencies supported a project for the ex situ conservation of bryophytes. Living and cryopreserved collections of UK threatened species were successfully established and the cryopreserved collection continues to be maintained. Other in vitro conservation collections are maintained over Europe, at botanic gardens, museums and by individual university researchers, but there is no coherent European collection of bryophytes for conservation, or standardisation of techniques. A major issue for many in vitro collections is the maintenance of within species genetic diversity. Such diversity is considered to be important, as it is the basis by which populations of species can adapt to new conditions and evolve. We are proposing to establish a European network for in vitro conservation of bryophytes. We envisage that this will include living collections, cryopreserved collections and spore collections. Conservation of genetic diversity would be a priority and the collections would provide a valuable resource for conservation initiatives and support research into rare and threatened species.

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Section: In Vitro Culture Of Bryophytesmentioning

confidence: 90%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…Bryophytes comprise three taxonomically distinct groups: liverworts, hornworts and mosses. A report by Takio et al (1986) is so far the only one that deals with growth characteristics of cell suspension cultures of moss plants. From the moss, Barbula unguiculata, we isolated two stable cell lines that can grow as suspension cultures: one cell line utilizes both nitrate and ammonia, while the other utilizes only ammonia.…”

Section: Introductionmentioning

confidence: 99%

Establishment and growth characterization of suspension culture of cells from the moss, Sphagnum imbrication

Kajita

Takio

Takami

et al. 1987

Physiologia Plantarum

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A green‐pigmented callus of the moss, Sphagnum imbricatum Hornsch. ex. Russ., was induced and a chlorophyllous cell suspension culture was established using a modified Murashige and Skoog's medium without plant hormones. Cell growth in the light in the presence of glucose started after a short lag and was exponential for 12 days. The chlorophyll level was about 15 μg (mg cell dry weight)−1 and photosynthetic activity ca 20 to 50 μmol O2 (mg chlorophyll)−1h−1. Cell growth in the light was negligible in the absence of glucose under ordinary air, but photoautotrophic growth was possible under elevated CO2 concentrations. In the dark, the moss cells grew heterotrophically and continued to synthesize chlorophyll, although at a much reduced rate. The suspension‐cultured cells redifferentiated protonemata and shoots when transferred to solid Knop's medium. In contrast to the callus cells, which could not assimilate nitrate, redifferentiated plantlets could use nitrate as the sole nitrogen source.

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“…Calluses were induced by culturing on solid MS medium (Murashige and Skoog 1962) with 1 mM 2,4-D (2,4-dichlorophenoxyacetic acid), 4% glucose, 0.1% CaCO 3 and 0.3% Gellan Gum (Wako Pure Chemical Industries, Ltd.). Induced calluses were cultured in liquid ASMS medium (Takio et al 1986) with 1 mM 2,4-D, 4% glucose and 20 mM sodium succinate on a gyratory shaker at 130 rpm and 25°C in continuous light (2,000 lux). Media pH was adjusted to 6.0 before autoclaving.…”

Section: Plant Materialsmentioning

confidence: 99%

Inhibition of Plastid Division by Ampicillin in the Pteridophyte Selaginella nipponica Fr. et Sav.

Izumi

Ono

Takano

2003

Plant and Cell Physiology

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;We investigated the effect of the b-lactam antibiotic, ampicillin, on plastid division in the pteridophyte Selaginella nipponica. Guard cells of plantlets treated with 1 mM ampicillin only often had one plastid, whereas guard cells of untreated plantlets had two to four plastids. We generated a S. nipponica cell culture system and used it to investigate the effects of ampicillin. Treatment with 1 mM ampicillin had no effect on cell division in culture. We classified cultured cells into four types based on the number of plastids they contained: one (Type I), two (Type II), three or four (Type III) and more than five (Type IV). After 3 d in culture, the percentage of each cell type (I-IV) was 29.5, 46.7, 20.9, and 1.9%, respectively. Subsequently, the percentage of Types III and IV increased gradually, reaching 61.9 and 11.4%, respectively, after 15 d in culture in the absence of ampicillin. When 1 mM ampicillin was added, there was a minimal increase in the number of Type III and IV cells, with high percentages of Type I and II cells (32.4 and 45.7%, respectively) after 15 d. These results suggest that ampicillin inhibits plastid division in S. nipponica.

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Photoautotrophic growth in suspension culture of cells from the moss, Barbula unguiculata

Cited by 10 publications

References 17 publications

In vitro conservation of European bryophytes

In vitro conservation of European bryophytes

Establishment and growth characterization of suspension culture of cells from the moss, Sphagnum imbrication

Inhibition of Plastid Division by Ampicillin in the Pteridophyte Selaginella nipponica Fr. et Sav.

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