Photoautotrophic hydrogen production of Rhodobacter sphaeroides in a microbial electrosynthesis cell

Li, Shuwei; Sakuntala, Mutyala; Song, Young Eun; Heo, Ji-ook; Kim, Min‐Soo; Lee, Soo-Youn; Kim, Min‐Sik; Oh, You-Kwan; Kim, Eun Jung

doi:10.1016/j.biortech.2020.124333

Cited by 37 publications

(10 citation statements)

References 26 publications

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“…Biohybrid electrochemical systems, where biological catalysts are coupled to abiotic electrodes, represent a sustainable approach for a variety of technological applications spanning from biosensing and water quality monitoring, − bioelectrosynthesis, − and micro to low power generation. − Additionally, the use of photosynthetic entities as the biocatalyst allows utilizing sunlight, one of the most attractive energy sources, to power such systems, paving the way to the field of semiartificial photosynthesis. − Using whole, metabolically active, microorganisms greatly simplifies the preparation of the biocatalyst (no enzyme isolation/purification required) and potentially enhances stability of the system thanks to their self-repairing and replication features. Purple nonsulfur bacteria have been used as model organisms for studying bacterial photosynthesis. , Additionally, purple bacteria are of great interest for their potential application for H 2 synthesis, , as well as bioremediation and biosensing, with Rhodobacter capsulatus ( R. capsulatus ), representing a very interesting candidate as biophotocatalyst due to their extreme metabolic versatility . However, the cell membranes of purple bacteria, and microorganisms in general, act as insulating material hindering the transfer of electrons from their redox active sites to the electrode surface .…”

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

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Interfacing intact and metabolically active photosynthetic bacteria with abiotic electrodes requires both establishing extracellular electron transfer and immobilizing the biocatalyst on electrode surfaces. Artificial approaches for photoinduced electron harvesting through redox polymers reported in literature require the separate synthesis of artificial polymeric matrices and their subsequent combination with bacterial cells, making the development of biophotoanodes complex and less sustainable. Herein, we report a one-pot biocompatible and sustainable approach, inspired by the byssus of mussels, that provides bacterial cells adhesion on multiple surfaces under wet conditions to obtain biohybrid photoanodes with facilitated photoinduced electron harvesting. Purple bacteria were utilized as a model organism, as they are of great interest for the development of photobioelectrochemical systems for H 2 and NH 3 synthesis, biosensing, and bioremediation purposes. The polydopamine matrix preparation strategy allowed the entrapment of active purple bacteria cells by initial oxygenic polymerization followed by electrochemical polymerization. Our results unveil that the deposition of bacterial cells with simultaneous polymerization of polydopamine on the electrode surface enables a 5-fold enhancement in extracellular electron transfer at the biotic/abiotic interface while maintaining the viability of the cells. The presented approach paves the way for a more sustainable development of biohybrid photoelectrodes.

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Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

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“…35 μm) on graphite cathodes, resulting in higher current consumption (≥10-fold) than that of the wild type. Recently, Li et al reported that R. sphaeroides produces H2 with its biomass growth through direct electron transfer from cathode to bacteria in the MES reactor [7]. Taken together, these may reflect that cathode modification using chitosan/carbodiimide composite may facilitate electron utilization by improving direct contact between an electrode and R. sphaeroides.…”

Section: Enhanced Co 2 Conversion By Modified Cathodesmentioning

confidence: 99%

“…Rhodobacter sphaeroides, a CO 2 -fixing chemoautotrophic bacterium, can catalyze both the production and consumption of hydrogen molecules (H 2 ) [6]. In a recent investigation, MES-driven CO 2 uptake and H 2 production in R. sphaeroides were found to occur simultaneously without additional organic carbon substrates [7].…”

Section: Introductionmentioning

confidence: 99%

“…2021, 11, x FOR PEER REVIEW 2 of 10 [6]. In a recent investigation, MES-driven CO2 uptake and H2 production in R. sphaeroides were found to occur simultaneously without additional organic carbon substrates [7]. Extracellular electron uptake mechanisms of electrotrophs from electrodes rely on: (i) indirect electron uptake by H2 or electron shuttles (i.e., natural and artificial redox mediators) or (ii) direct electron uptake via physical contact through electron-transfer proteins or apparatus [1,8].…”

Section: Introductionmentioning

confidence: 99%

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Surface Modification of a Graphite Felt Cathode with Amide-Coupling Enhances the Electron Uptake of Rhodobacter sphaeroides

Fitriana

Lee

et al. 2021

Applied Sciences

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Microbial electrosynthesis (MES) is a promising technology platform for the production of chemicals and fuels from CO2 and external conducting materials (i.e., electrodes). In this system, electroactive microorganisms, called electrotrophs, serve as biocatalysts for cathodic reaction. While several CO2-fixing microorganisms can reduce CO2 to a variety of organic compounds by utilizing electricity as reducing energy, direct extracellular electron uptake is indispensable to achieve highly energy-efficient reaction. In the work reported here, Rhodobacter sphaeroides, a CO2-fixing chemoautotroph and a potential electroactive bacterium, was adopted to perform a cathodic CO2 reduction reaction via MES. To promote direct electron uptake, the graphite felt cathode was modified with a combination of chitosan and carbodiimide compound. Robust biofilm formation promoted by amide functionality between R. sphaeroides and a graphite felt cathode showed significantly higher faradaic efficiency (98.0%) for coulomb to biomass and succinic acid production than those of the bare (34%) and chitosan-modified graphite cathode (77.8%), respectively. The results suggest that cathode modification using a chitosan/carbodiimide composite may facilitate electron utilization by improving direct contact between an electrode and R. sphaeroides.

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“…Because of its high metabolic versatility ( e.g., photoautotrophic, photoheterotrophic and chemoheterotrophic), a variety of value-added products, such as coenzyme Q 10 (Q 10 ) [ [10] , [11] , [12] , [13] ], isoprenoids ( i.e. , lycopene and β-carotene) [ [14] , [15] , [16] , [17] ], 5-amino-ketone pentyl acid [ 18 ] and even bio-hydrogen [ 19 , 20 ] can be efficiently produced in this cell factory. Therefore, developing a synthetic biology toolbox for R. sphaeroides would facilitate refactoring of synthetic pathways for bioproduct production and further expand the biotechnology application potential of R. sphaeroides .…”

Section: Introductionmentioning

confidence: 99%

Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides

Shi

Zhang

Liang

et al. 2021

Synthetic and Systems Biotechnology

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The versatile photosynthetic α-proteobacterium Rhodobacter sphaeroides , has recently been extensively engineered as a novel microbial cell factory (MCF) to produce pharmaceuticals, nutraceuticals, commodity chemicals and even hydrogen. However, there are no well-characterized high-activity promoters to modulate gene transcription during the engineering of R. sphaeroides . In this study, several native promoters from R. sphaeroides JDW-710 (JDW-710), an industrial strain producing high levels of co-enzyme Q 10 (Q 10 ) were selected on the basis of transcriptomic analysis. These candidate promoters were then characterized by using gusA as a reporter gene. Two native promoters, P rsp _ 7571 and P rsp _ 6124 , showed 620% and 800% higher activity, respectively, than the tac promoter, which has previously been used for gene overexpression in R. sphaeroides. In addition, a P rsp _ 7571 -derived synthetic promoter library with strengths ranging from 54% to 3200% of that of the tac promoter, was created on the basis of visualization of red fluorescent protein (RFP) expression in R. sphaeroides . Finally, as a demonstration, the synthetic pathway of Q 10 was modulated by the selected promoter T334* in JDW-710; the Q 10 yield in shake-flasks increased 28% and the production reached 226 mg/L . These well-characterized promoters should be highly useful in current synthetic biology platforms for refactoring the biosynthetic pathway in R. sphaeroides -derived MCFs.

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Photoautotrophic hydrogen production of Rhodobacter sphaeroides in a microbial electrosynthesis cell

Cited by 37 publications

References 26 publications

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Surface Modification of a Graphite Felt Cathode with Amide-Coupling Enhances the Electron Uptake of Rhodobacter sphaeroides

Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides

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