2022
DOI: 10.1002/cssc.202201275
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Photobiocatalytic Decarboxylation for the Synthesis of Fatty Epoxides from Renewable Fatty Acids

Abstract: Fatty epoxides are unique building blocks in organic transformations and materials production; however, their synthetic methodologies are currently not accessible from renewable fatty acids. Herein, a photoenzymatic decarboxylation of epoxy fatty acids into fatty epoxides was demonstrated using fatty acid photodecarboxylase (FAP) from Chlorella variabilis NC64A (CvFAP). Various fatty epoxides were synthesized in excellent selectivity by wild‐type CvFAP. The decarboxylation reaction was also achieved with four … Show more

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Cited by 9 publications
(3 citation statements)
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“…Overall, our results demonstrate that cell-free system is feasible to express the photoenzyme CvFAP with catalytic activity. Since its discovery in 2017, CvFAP has been quickly employed for different enzymatic bioconversions ( Ge et al, 2022 ; Li et al, 2021 ; Xu et al, 2019 ; Zeng et al, 2021 ; Zhang et al, 2019 ). Therefore, we envision that CFPS will not only can be used for the construction of CvFAP-based metabolic pathway(s) to synthesize various products in vitro , but also may serve as a rapid method to engineer, express, and evaluate CvFAP variants if the design-build-test cycles performed in cells are laborious and time-consuming.…”
Section: Resultsmentioning
confidence: 99%
“…Overall, our results demonstrate that cell-free system is feasible to express the photoenzyme CvFAP with catalytic activity. Since its discovery in 2017, CvFAP has been quickly employed for different enzymatic bioconversions ( Ge et al, 2022 ; Li et al, 2021 ; Xu et al, 2019 ; Zeng et al, 2021 ; Zhang et al, 2019 ). Therefore, we envision that CFPS will not only can be used for the construction of CvFAP-based metabolic pathway(s) to synthesize various products in vitro , but also may serve as a rapid method to engineer, express, and evaluate CvFAP variants if the design-build-test cycles performed in cells are laborious and time-consuming.…”
Section: Resultsmentioning
confidence: 99%
“…Each discipline of photobiocatalysis offers opportunities with a varied degree of associated challenges. Although the number of known natural PEs (Figure 1a) is low at the moment, the recent discovery of a new PE-class, namely FAPs [9,[93][94] indicates that there might be more to come, especially as natural light-dependent enzymes were not in the focus of research until recently. Such new enzymes would again trigger an avalanche of opportunities.…”
Section: Outlook Of Using Light In Enzymatic Systemsmentioning
confidence: 99%
“…GFP11 and a GS Linker (DGGSGGGS) was introduced in the primer while amplifying CvFAP gene. CvFAP gene was amplified with Zhl_Oli819 and Zhl_Oli820 using a plasmid containing CvFAP gene as the template [6c] . The vector was amplified with Zhl_Oli823 and Zhl_Oli824 using pCDFDuet (Novagen, V011038#) as the template.…”
Section: Experiments Sectionmentioning
confidence: 99%