Photobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regeneration

Çalış, Mert; Irmak, Gülseren; Demirtaş, Tuǧrul Tolga; Kara, Murat; Üstün, Galip Gencay; Gümüşderelı́oğlu, Menemşe; Türkkanı, Ayten; Çakar, Ayşe Nur; Özgür, Figen

doi:10.1007/s10103-021-03308-y

Cited by 17 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Near-infrared light exposure enhanced matrix mineralization, with a 79.93 ± 3.41% mineralization rate in the PBM-treated group compared to 60.62 ± 6.34% in the non-PBM group. In vivo implantation of the hydrogels in calvarial bone defects showed increased bone regeneration and osteogenic differentiation of ADSCs with PBM . These effects may be attributed to improved ATP and nitric oxide production within the cells due to light-induced mitochondrial respiratory chain reactions.…”

Section: Integration Of Additives To Improve the Osteogenic Potential...mentioning

confidence: 93%

Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities

Mamidi,

Ijadi,

Norahan

2023

Biomacromolecules

View full text Add to dashboard Cite

The field of bone tissue engineering has seen significant advancements in recent years. Each year, over two million bone transplants are performed globally, and conventional treatments, such as bone grafts and metallic implants, have their limitations. Tissue engineering offers a new level of treatment, allowing for the creation of living tissue within a biomaterial framework. Recent advances in biomaterials have provided innovative approaches to rebuilding bone tissue function after damage. Among them, gelatin methacryloyl (GelMA) hydrogel is emerging as a promising biomaterial for supporting cell proliferation and tissue regeneration, and GelMA has exhibited exceptional physicochemical and biological properties, making it a viable option for clinical translation. Various methods and classes of additives have been used in the application of GelMA for bone regeneration, with the incorporation of nanofillers or other polymers enhancing its resilience and functional performance. Despite promising results, the fabrication of complex structures that mimic the bone architecture and the provision of balanced physical properties for both cell and vasculature growth and proper stiffness for load bearing remain as challenges. In terms of utilizing osteogenic additives, the priority should be on versatile components that promote angiogenesis and osteogenesis while reinforcing the structure for bone tissue engineering applications. This review focuses on recent efforts and advantages of GelMA-based composite biomaterials for bone tissue engineering, covering the literature from the last five years.

show abstract

Section: Integration Of Additives To Improve the Osteogenic Potential...mentioning

confidence: 93%

Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities

Mamidi,

Ijadi,

Norahan

2023

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…NIR-responsive biomaterials in particular improved bone metabolism by activating pathways through enhanced oxidative metabolism in the mitochondria [ 119 ]. In addition, NIR leads to mild localized heat and a photoelectronic microenvironment that can induce osteogenic differentiation [ 120 , 121 ]. For example, an NIR-responsive hydrogel composed of methyl methacrylate, GelMA, and polydopamine nanoparticles was prepared using a free-radical polymerization method to study photothermal therapy for skull healing [ 122 ].…”

Section: Electromagnetic Radiation Smart Hydrogels For Bone Regenerationmentioning

confidence: 99%

Smart Hydrogels for Bone Reconstruction via Modulating the Microenvironment

et al. 2023

View full text Add to dashboard Cite

Rapid and effective repair of injured or diseased bone defects remains a major challenge due to shortages of implants. Smart hydrogels that respond to internal and external stimuli to achieve therapeutic actions in a spatially and temporally controlled manner have recently attracted much attention for bone therapy and regeneration. These hydrogels can be modified by introducing responsive moieties or embedding nanoparticles to increase their capacity for bone repair. Under specific stimuli, smart hydrogels can achieve variable, programmable, and controllable changes on demand to modulate the microenvironment for promoting bone healing. In this review, we highlight the advantages of smart hydrogels and summarize their materials, gelation methods, and properties. Then, we overview the recent advances in developing hydrogels that respond to biochemical signals, electromagnetic energy, and physical stimuli, including single, dual, and multiple types of stimuli, to enable physiological and pathological bone repair by modulating the microenvironment. Then, we discuss the current challenges and future perspectives regarding the clinical translation of smart hydrogels.

show abstract

“…Some studies have proved that ASCs combined with physical stimulation therapy is effective. In the rat bone defect with Frontiers in Bioengineering and Biotechnology frontiersin.org biparietal critical-sized bone defects (6 mm in size), the use of photobiomodulation (PBM) combined with ASCs wrapped in methacrylic acid gelatin hydrogel improved bone regeneration in vivo (Calis et al, 2021). Moreover, low-power laser irradiation combined with ASCs significantly enhanced the process of bone healing in the rat model of skull defect (Wang et al, 2018b).…”

Section: Physical and Chemical Stimulationmentioning

confidence: 99%

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

Zhang

Yang

Cao

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

In the field of orthopaedics, bone defects caused by severe trauma, infection, tumor resection, and skeletal abnormalities are very common. However, due to the lengthy and painful process of related surgery, people intend to shorten the recovery period and reduce the risk of rejection; as a result, more attention is being paid to bone regeneration with mesenchymal stromal cells, one of which is the adipose-derived mesenchymal stem cells (ASCs) from adipose tissue. After continuous subculture and cryopreservation, ASCs still have the potential for multidirectional differentiation. They can be implanted in the human body to promote bone repair after induction in vitro, solve the problems of scarce sources and large damage, and are expected to be used in the treatment of bone defects and non-union fractures. However, the diversity of its differentiation lineage and the lack of bone formation potential limit its current applications in bone disease. Here, we concluded the current applications of ASCs in bone repair, especially with the combination and use of physical and biological methods. ASCs alone have been proved to contribute to the repair of bone damage in vivo and in vitro. Attaching to bone scaffolds or adding bioactive molecules can enhance the formation of the bone matrix. Moreover, we further evaluated the efficiency of ASC-committed differentiation in the bone in conditions of cell experiments, animal models, and clinical trials. The results show that ASCs in combination with synthetic bone grafts and biomaterials may affect the regeneration, augmentation, and vascularization of bone defects on bone healing. The specific conclusion of different materials applied with ASCs may vary. It has been confirmed to benefit osteogenesis by regulating osteogenic signaling pathways and gene transduction. Exosomes secreted by ASCs also play an important role in osteogenesis. This review will illustrate the understanding of scientists and clinicians of the enormous promise of ASCs’ current applications and future development in bone repair and regeneration, and provide an incentive for superior employment of such strategies.

show abstract

Photobiomodulation combined with adipose-derived stem cells encapsulated in methacrylated gelatin hydrogels enhances in vivo bone regeneration

Cited by 17 publications

References 50 publications

Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities

Leveraging the Recent Advancements in GelMA Scaffolds for Bone Tissue Engineering: An Assessment of Challenges and Opportunities

Smart Hydrogels for Bone Reconstruction via Modulating the Microenvironment

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

Contact Info

Product

Resources

About