Photobiomodulation combined with photodynamic therapy using ruthenium phthalocyanine complexes in A375 melanoma cells: Effects of nitric oxide generation and ATP production

Negri, Laísa Bonafim; Martins, Tássia Joi; Silva, Roberto Santana da; Hamblin, Michael R.

doi:10.1016/j.jphotobiol.2019.111564

Cited by 39 publications

(17 citation statements)

References 42 publications

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“…[1][2][3][4][5][6][7] Photodynamic therapy (PDT) is an emerging non-invasive technique based on the use of light, oxygen, and photosensitizing molecules to produce reactive oxygen species (ROS) for killing cancer and/or bacterial cells. [8][9][10][11][12] In particular, efficient PDT relies on the generation of sufficient ROS, especially singlet oxygen ( 1 O 2 ), to be able to disrupt the 'stubborn' microbial and cancerous cells. [13][14][15] Although some of the conventional photosensitizers are also fluorescent molecules, most of them are not soluble in water, making them easily aggregate at high concentrations in the biological medium due to p-p stacking.…”

Section: Introductionmentioning

confidence: 99%

Nanosilver-enhanced AIE photosensitizer for simultaneous bioimaging and photodynamic therapy

Yaraki

Pan

et al. 2020

Mater. Chem. Front.

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Section: Introductionmentioning

confidence: 99%

Nanosilver-enhanced AIE photosensitizer for simultaneous bioimaging and photodynamic therapy

Yaraki

Pan

et al. 2020

Mater. Chem. Front.

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“…Chamani and Zadlo et al found that apoptosis and cell cycle arrest in melanoma cells was significantly induced using photodynamic therapy (PDT) 87,88 . Hamblin et al found that treatment of A375 melanoma cells with PBM combined with photodynamic therapy further reduced cell activity more effectively 89 . In addition to PBM and PDT, it is worth noting that PBM can also be combined with gene editing and optogenetics to improve the efficiency of treating melanoma.…”

Section: Future Studiesmentioning

confidence: 99%

The review of the light parameters and mechanisms of Photobiomodulation on melanoma cells

Chen

Huang

Liu

2021

Photoderm Photoimm Photomed

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Photobiomodulation (PBM) uses low‐intensity visible or near‐infrared light to produce beneficial effects on cells or tissues, such as brain therapy, wound healing. Still there is no consistent recommendation on the parameters (dose, light mode, wavelength, irradiance) and protocols (repetition, treatment duration) for its clinical application. Herein, we summarize the current PBM parameters for the treatment of melanoma, and we also discuss the potential photoreceptors and downstream signaling mechanisms in the PBM treatment of melanoma cells. It is hypothesized that PBM may inhibit the melanoma cells by activating mitochondria, OPNs, and other receptors. Regardless of the underlying mechanisms, PBM has been shown to be beneficial in treating melanoma. Through further in‐depth studies of the underlying potential mechanisms, it can strengthen the applications of PBM for the therapy of melanoma.

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“…Photodynamic therapy (PDT) is a non-invasive approach to treat oncological and non-oncological diseases (Figueira et al, 2014;Kwiatkowski et al, 2018;Mesquita et al, 2018a,b;Gazzi et al, 2019;Negri et al, 2019;Castro et al, 2020;Gomes et al, 2020;Lee et al, 2020). PDT is a clinically approved treatment based on the activation of a photosensitizer (PS) by the light of a specific wavelength, in the presence of molecular oxygen to produce reactive oxygen species (ROS), resulting in neoplastic cell death (Agostinis et al, 2011;Bogoeva et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticles as an Efficient Porphyrin Delivery System for Cancer Cells to Enhance Photodynamic Therapy

Silva

Castro

Botteon

et al. 2021

Front. Bioeng. Biotechnol.

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Photodynamic therapy (PDT) is a potential non-invasive approach for application in oncological diseases, based on the activation of a photosensitizer (PS) by light at a specific wavelength in the presence of molecular oxygen to produce reactive oxygen species (ROS) that trigger the death tumor cells. In this context, porphyrins are interesting PS because they are robust, have high chemical, photo, thermal, and oxidative stability, and can generate singlet oxygen (1O2). However, porphyrins exhibit low solubility and a strong tendency to aggregate in a biological environment which limits their clinical application. To overcome these challenges, we developed hybrid nanostructures to immobilize 5,10,15,20-tetrakis[(4-carboxyphenyl) thio-2,3,5,6-tetrafluorophenyl] (P), a new third-generation PS. The biological effect of this system was evaluated against bladder cancer (BC) cells with or without light exposition. The nanostructure composed of lipid carriers coated by porphyrin-chitosan (P-HNP), presented a size of ca. 130 nm and low polydispersity (ca. 0.25). The presence of the porphyrin-chitosan (P-chitosan) on lipid nanoparticle surfaces increased the nanoparticle size, changed the zeta potential to positive, decreased the recrystallization index, and increased the thermal stability of nanoparticles. Furthermore, P-chitosan incorporation on nanoparticles increased the stability and enhanced the self-organization of the system and the formation of spherical structures, as observed by small-angle X-ray scattering (SAXS) analysis. Furthermore, the immobilization process maintained the P photoactivity and improved the photophysical properties of PS, minimizing its aggregation in the cell culture medium. In the photoinduction assays, the P-HNP displayed high phototoxicity with IC50 3.2-folds lower than free porphyrin. This higher cytotoxic effect can be correlated to the high cellular uptake of porphyrin immobilized, as observed by confocal images. Moreover, the coated nanoparticles showed mucoadhesive properties interesting to its application in vivo. Therefore, the physical and chemical properties of nanoparticles may be relevant to improve the porphyrin photodynamic activity in BC cells.

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Photobiomodulation combined with photodynamic therapy using ruthenium phthalocyanine complexes in A375 melanoma cells: Effects of nitric oxide generation and ATP production

Cited by 39 publications

References 42 publications

Nanosilver-enhanced AIE photosensitizer for simultaneous bioimaging and photodynamic therapy

Nanosilver-enhanced AIE photosensitizer for simultaneous bioimaging and photodynamic therapy

The review of the light parameters and mechanisms of Photobiomodulation on melanoma cells

Hybrid Nanoparticles as an Efficient Porphyrin Delivery System for Cancer Cells to Enhance Photodynamic Therapy

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