Photobiomodulation restores spermatogenesis in the transient scrotal hyperthermia-induced mice

Hasani, Amirhosein; Khosravi, Amir; Rahimi, Kimia; Afshar, Azar; Fadaei-Fathabadi, Fatemeh; Raoofi, Amir; Raee, Pourya; Aghajanpour, Fakhroddin; Aliaghaei, Abbas; Abdi, Shabnam; Norouzian, Mohsen; Abdollahifar, Mohammad‐Amin

doi:10.1016/j.lfs.2020.117767

Cited by 29 publications

(24 citation statements)

References 44 publications

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“…Based on the Western blot and real-time PCR analysis, as compared to the control group, a remarkable increase was observed in protein expression levels of cell death markers such as Caspase-1, Bclin-1, Atg7, Mlkl and Acsl4, suggesting that scrotal hyperthermia can induce cell death by using pyroptosis, autophagy, necroptosis and ferroptosis signalling pathway. We found that testicular heating not only induces apoptosis (Hasani et al, 2020;Ilkhani et al, 2020), but also induces pyroptosis, autophagy, necroptosis…”

Section: Discussionmentioning

confidence: 80%

“…Germ cell apoptosis is a potential mechanism responsible for the destruction of spermatogenesis induced by testicular hyperthermia (Wang et al, 2007). Our previous research showed that scrotal hyperthermia could notably impair the sperm parameters and histological parameters; it can also decrease serum testosterone level and GSH activity and increase ROS production and expression of inflammatory markers like IL1, IL6 and TNFα genes (Hasani et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Pyroptosis is a form of cell death that is triggered by caspase-1 and the secretion of proinflammatory cytokines, causing karyokinesis, cell oedema and plasma membrane rupture, and eventually cell death (Sarhan et al, 2018). Oxidative stress can lead to different types of cell death (Hasani et al, 2020), so it is important to evaluate a standard panel of biomarkers and functional tests related to non-apoptotic cell death signalling pathways, to accurately distinguish between different forms of cell death that may occur in tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Increased testicular temperature induces germ cell depletion and reduces spermatogenesis and fertility. Also, testicular hyperthermia leads to oligospermia and azoospermia (Hasani et al, 2020;Ilkhani et al, 2020;Khosravi et al, 2021;Ziaeipour et al, 2019). Raised testicular temperature causes the atrophy of germinal epithelium and spermatogenic arrest (Ziaeipour et al, 2019), leading to lower sperm counts; Sertoli (Cai et al, 2011) and Leydig (Kanter & Aktas, 2009) cells are also impacted by heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Non‐apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice

et al. 2021

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There are two types of cell death, one is accidental cell death (ACD) and the other is regulated cell death (RCD). ACD is a biologically uncontrollable process caused by disease, while RCD includes welltuned and structured signalling cascades defined as effective molecular mechanisms (Tang et al., 2019). The scientific observation in RCD research started when researchers introduced and defined the terms apoptosis, autophagy, necroptosis, ferroptosis and pyroptosis. Cell death may occur in response to oxidative stress, which activates signalling pathways in multiple forms and can therefore contribute to human diseases such as cancer, tissue damage, autoimmune and inflammatory diseases (Tang et al., 2019). Apoptosis can activate caspase nucleus oligo DNA fragmentation eventually

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Non‐apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice

et al. 2021

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“…ROS is of great significance in maintaining the homeostasis of the body’s physiological environment. Accumulation of excess ROS causes oxidative stress and cell apoptosis [ 60 ]. SOD is an important scavenger of ROS, which prevents tissue damage caused by free radical reaction [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Liu¹,

Liu²,

Hu³

et al. 2022

IJMS

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Quercetin and kaempferol are flavonoids widely present in fruits, vegetables, and medicinal plants. They have attracted much attention due to their antioxidant, anti-inflammatory, anticancer, antibacterial, and neuroprotective properties. As the guarantee cells in direct contact with germ cells, Sertoli cells exert the role of support, nutrition, and protection in spermatogenesis. In the current study, network pharmacology was used to explore the targets and signaling pathways of quercetin and kaempferol in treating spermatogenic disorders. In vitro experiments were integrated to verify the results of quercetin and kaempferol against heat stress-induced Sertoli cell injury. The online platform was used to analyze the GO biological pathway and KEGG pathway. The results of the network pharmacology showed that quercetin and kaempferol intervention in spermatogenesis disorders were mostly targeting the oxidative response to oxidative stress, the ROS metabolic process and the NFκB pathway. The results of the cell experiment showed that Quercetin and kaempferol can prevent the decline of cell viability induced by heat stress, reduce the expression levels of HSP70 and ROS in Sertoli cells, reduce p-NF-κB-p65 and p-IκB levels, up-regulate the expression of occludin, vimentin and F-actin in Sertoli cells, and protect cell structure. Our research is the first to demonstrate that quercetin and kaempferol may exert effects in resisting the injury of cell viability and structure under heat stress.

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Photobiomodulation therapy reverses spermatogenesis arrest in hyperthermia-induced azoospermia mouse model

et al. 2023

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Photobiomodulation restores spermatogenesis in the transient scrotal hyperthermia-induced mice

Cited by 29 publications

References 44 publications

Non‐apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice

Non‐apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Photobiomodulation therapy reverses spermatogenesis arrest in hyperthermia-induced azoospermia mouse model

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