Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic β-glucosidases with industrial potential

Araújo, Juscemácia N.; Tofanello, Aryane; Silva, Viviam M. da; Sato, Juliana Alves Pereira; Squina, Fábio M.; Nantes, Iseli L.; Garcia, Wânius

doi:10.1016/j.ijbiomac.2017.04.001

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…This indicated the formation of AgCl‐NPs, as was confirmed by the surface plasmon resonance peak at 435 nm analyzed from UV–vis absorption spectra (Fig. B) . The absorption peak in the range of 400–500 nm indicated that AgCl‐NPs could be fabricated .…”

Section: Resultsmentioning

confidence: 52%

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Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities

Siritapetawee

Limphirat

Nantapong

et al. 2018

Biotech and App Biochem

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Recently, the development of "green" methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility, and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) in phosphate-buffered saline (pH 7.2), under the influence of visible light. The fabricated nanoparticles had a maximal surface plasmon resonance absorption peak at 435 nm. The size of the AgCl-NPs, estimated by scanning electron microscopy, was 57 ± 14.7 nm. Energy dispersive X-ray spectroscopy, X-ray absorption spectroscopy, and X-ray diffraction analysis confirmed that the fabricated Ag-NPs were of the AgCl type. The fabricated nanoparticles had antioxidant activity, scavenging DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals with IC of 204 ± 1.8 μg/mL. The fabricated AgCl-NPs had broad-spectrum in vitro antimicrobial activities, acting against the Gram-positive bacteria Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and Bacillus cereus, and the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. AgCl-NPs also showed antifungal activity against Candida albicans and C. tropicalis. In addition, AgCl-NPs showed antiprotozoal activity against Giardia lamblia, with IC 202 ± 2.1 μg/mL. Based on the biological activities of the fabricated AgCl-NPs, they have the potential for widespread application in medicine and industry.

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Section: Resultsmentioning

confidence: 52%

“…B) . The absorption peak in the range of 400–500 nm indicated that AgCl‐NPs could be fabricated . The amount of fabricated AgCl‐NPs increased with increasing concentration of the protease, as shown by increased UV–vis absorbance with the same peak at 435 nm (Fig.…”

Section: Resultsmentioning

confidence: 86%

Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities

Siritapetawee

Limphirat

Nantapong

et al. 2018

Biotech and App Biochem

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“…At left, the flask with the brownish solution is the seed suspension used for preparing the NRs. Other promising metallic nanostructures for the fabrication of materials with virucidal properties are the Ag/Halogens-NPs (Araújo et al 2017, Cruz et al 2018) that exhibited efficient photocatalytic activity on the removal of dyes. Considering that the bleaching of dyes is associated with the production of reactive oxygen species, these nanoparticles are promising for virus inactivation.…”

Section: Gold and Silver Nanoparticlesmentioning

confidence: 99%

Promising Nanostructured Materials against Enveloped Virus

Toledo

Lanfredi

et al. 2020

An. Acad. Bras. Ciênc.

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“…Some accounts about the effect of nanoparticles on luciferase activity have been restricted to the changes of the enzyme structure modulated by association with nanostructures. The association of enzymes to nanoparticles can provide several (Araújo et al 2017, 2018). The enzymes β-glucosidases have many processes and industrial applications.…”

Section: Enzyme-functionalized Nanostructures Of Noble Metalsmentioning

confidence: 99%

Proteins and Peptides at the Interfaces of Nanostructures

Brito

Belleti

Menezes

et al. 2019

An. Acad. Bras. Ciênc.

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The present review focuses on the proteins and peptides at the interfaces of nanostructured metals and semiconductors as a result of their use in synthesis in-situ and functionalization of nanostructures. We start the review with an introduction about the peculiar properties of nanostructured materials and their applications. In the following, the chemical and structural properties of peptides and proteins that allow their use as reducing, stabilizing, and functionalization agents are discussed. Proteins and peptides have not only the chemical groups for the metal ion reducing but also provide templates for directing the crystalline growing of nanostructures to the desired shapes and sizes. Proteins and peptides are also used mainly for the stabilization and functionalization of a diversity of nanostructured materials providing properties such as biocompatibility, plasmon-enhanced catalysis, sensing, micro/nanomotors, spin filters, and others. Nanostructured materials of metal oxides have mainly been functionalized with proteins and peptides to gain specific properties such as light harvesting and spin filters. Herein, we described the synthesis and functionalization of some types of nanostructured materials by using peptides and proteins. In the last part of the review, it is discussed the perspectives and challenges for the use of proteins and peptides in Nanotechnology.

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Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic β-glucosidases with industrial potential

Cited by 15 publications

References 44 publications

Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities

Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities

Promising Nanostructured Materials against Enveloped Virus

Proteins and Peptides at the Interfaces of Nanostructures

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