Photocrosslinked gelatin hydrogel improves wound healing and skin flap survival by the sustained release of basic fibroblast growth factor

Abstract

Biomaterials traditionally used for wound healing can act as a temporary barrier to halt bleeding, prevent infection, and enhance regeneration. Hydrogels are among the best candidates for wound healing owing to their moisture retention and drug-releasing properties. Photo-polymerization using visible light irradiation is a promising method for hydrogel preparation since it can easily control spatiotemporal reaction kinetics and rapidly induce a single-step reaction under mild conditions. In this study, photocrosslinked gelatin hydrogels were imparted with properties namely fast wound adherence, strong wet tissue surface adhesion, greater biocompatibility, long-term bFGF release, and importantly, ease of use through the modification and combination of natural bio-macromolecules. The production of a gelatin hydrogel made of natural gelatin (which is superior to chemically modified gelatin), crosslinked by visible light, which is more desirable than UV light irradiation, will enable its prolonged application to uneven wound surfaces. This is due to its flexible shape, along with the administration of cell growth factors, such as bFGF, for tissue regeneration. Further, the sustained release of bFGF enhances wound healing and skin flap survival. The photocrosslinking gelatin hydrogel designed in this study is a potential candidate to enhance wound healing and better skin flap survival.

Smart Polycationic Hydrogel Dressing for Dynamic Wound Healing

“…A sterile and moist healing environment was studied to accelerate wound healing (Figure 6f). [ 53,54 ]…”

“…A sterile and moist healing environment was studied to accelerate wound healing (Figure 6f). [53,54] We further evaluated the wound healing effect by H&E and Masson staining (Figure 6d,e and Figure S12a,b, Supporting Information). The results showed that both the blank control and the NIR-group developed an acute inflammatory response after 3 d of treatment.…”

An injectable cellulose acetate/sodium alginate hydrogels-loaded laponite microsphere as a potential wound healing in nursing care in perioperative period

“…The quantifiable wound closure result is shown in figure 9(B), which shows that wounds treated with CSL had the greatest wound closure rate during the entire period [51]. On day 14, wounds treated with CSL were almost entirely closed (98.9±1.0%), whereas wounds treated with CA/SA had a much lower closure rate (82.6±4.1%) [52]. These results highlighted that the CSL exhibited much better acceleration effect on wound healing.…”

Programmable DNA Hydrogels as Artificial Extracellular Matrix

“…In native tissues, the 3D cellular microenvironments are highly heterogeneous, anisotropic, and dynamic, involving complex elements such as cell populations, extracellular matrix (ECM) components, soluble factors, and physical forces. [ 1–5 ] Among all these elements, ECM guarantees the main biophysical and biochemical cues for cells in vivo, [ 6–8 ] which are indispensable for cell spreading, proliferation, migration, differentiation, and function. [ 9–11 ] To recapitulate and modulate various components of native ECM, and consequently enable in vitro cellular studies in controlled models, artificial ECM engineering has attracted booming research interest in the past two decades.…”