Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells

Abstract

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

One-Step FRESH Bioprinting of Low-Viscosity Silk Fibroin Inks

“…Moreover, the decreased diameter of generated SF hydrogel constructs (Figure f) was attributed to an increase in β-sheet structures. This finding is consistent with a report by Yu et al indicating that a hydrogel containing SF shrunk and lost water because of an increase in β-sheet structures . Based on a report by Ribeiro et al indicating that the stiffness of hydrogels was increased through the conformational transition from random coil to β-sheet, the mechanical properties of cell-laden SF hydrogel constructs generated in this study would also change over time.…”

Preparation, Characterization, and In Vitro Release of Curcumin-Loaded IRMOF-10 Nanoparticles and Investigation of Their Pro-Apoptotic Effects on Human Hepatoma HepG2 Cells

“…Compared to the solvothermal method, this method can be performed at room temperature, which is safer and more convenient, and can rapidly synthesize MOFs in a short time [4]. To date, many materials have been used as carrier materials for curcumin, such as CUR-loaded solid lipid nanoparticles (37% ± 2.5%) [41], CUR-loaded chitosan nanoparticles (18%) [42], CUR-loaded mesoporous silica nanoparticles (MSNs), and CUR-loaded amine-functionalized MSNs (7.9%, 24.4%) [43]. The common drug-loading methods for MOFs include the solvent adsorption method [44], covalent crosslinking method [45], and an insitu growth method [46].…”

Self-assembled silk fibroin hydrogels: from preparation to biomedical applications

“…41 As-obtained regenerated aqueous fibroin solutions can be processed into several forms, such as films, 42 meshes, 43 sponges, 44 and hydrogels. [45][46][47] 1.4 Characteristics of SF as biomaterials SF derived from the B. mori, as a natural protein biopolymer, has been widely studied in biomedical applications, mainly due to its mechanical performance, tunable degradation ability, and its biocompatibility with various biological tissues such as skin and cartilage. 48,49 The proper balance of breaking strength, modulus, and elongation makes SF ductile and tough.…”

“…41 Asobtained regenerated aqueous fibroin solutions can be processed into several forms, such as films, 42 meshes, 43 sponges, 44 and hydrogels. [45][46][47] Figure 3. A) Schematic of silk fibroin regeneration process.…”