Photodynamic Action in Thin Sensitized Layers: Estimating the Utilization of Light Energy

Меерович, Г. А.; Romanishkin, I.D.; Akhlyustina, E. V.; Strakhovskaya, M. G.; Kogan, Evgeniya; Ангелов, Иван; Loschenov, Victor B.; Borisova, Ekaterina

doi:10.18287/jbpe21.07.040301

Cited by 5 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PSs used in this study were monocationic 3,7-bis-(Dimethylamino)-phenazathionium chloride C16H18ClN3S (methylene blue, Samaramedprom, Samara, Russia) and octacationic octakis(cholinyl)zinc phthalocyanine (Zn-PcChol 8+ , Organic Intermediates and Dyes Institute, Moscow, Russia). The stick models with equipotential electrostatic surfaces of MB, with a total charge of +1, and Zn-PcChol 8+ with a total charge of +8, are shown in Figure 1 , in accordance with our previous study [ 22 , 27 ].…”

Section: Methodssupporting

confidence: 90%

“… Cationic PSs used in this study and LEDs for their excitation: monocationic MB ( a , b ), octacationic Zn-PcChol 8+ ( c , d ); the stick models of PSs with equipotential electrostatic surfaces colored by red (−7 mV) and blue (+7 mV) in accordance with [ 22 ] ( a , c ); normalized spectral contours of the absorption bands of PSs (1) and normalized emission spectra of LED sources (2) in accordance with [ 27 ] ( b , d ). …”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Infectivity and Morphology of Bovine Coronavirus Inactivated In Vitro by Cationic Photosensitizers

Zhukhovitsky

Shevlyagina²,

Zubasheva³

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

Bovine coronaviruses (BCoVs), which cause gastrointestinal and respiratory diseases in cattle, and are genetically related to the human coronavirus HCoV-OC43, which is responsible for up to 10% of common colds, attract increased attention. We applied the method of photodynamic inactivation with cationic photosensitizers (PSs) to reduce the titers of BCoV and studied the morphological structure of viral particles under various modes of photodynamic exposure. The samples of virus containing liquid with an initial virus titer of 5 Log10 TCID50/mL were incubated with methylene blue (MB) or octakis(cholinyl)zinc phthalocyanine (Zn-PcChol8+) at concentrations of 1–5 μM for 10 min in the dark at room temperature. After incubation, samples were irradiated with LED (emission with maximum at 663 nm for MB or at 686 nm for Zn-PcChol8+) with light doses of 1.5 or 4 J/cm2. Next, the irradiation titrated virus containing liquid was studied using negative staining transmission electron microscopy. MB and Zn-PcChol8+ at concentrations of 1–5 μM, in combination with red light from LED sources in the low doses of 1.5–4.0 J/cm2, led to a decrease in BCoV titers by at least four orders of magnitude from the initial titer 5 Log10 TCID50/mL. Morphological changes in photodamaged BCoVs with increasing PS concentrations were loss of spikes, change in shape, decreased size of virus particles, destruction of the envelope, and complete disintegration of viruses. BCoV has been found to be sensitive to MB, which is the well-known approved drug, even in the absence of light.

show abstract

Section: Methodssupporting

confidence: 90%

Section: Figurementioning

confidence: 99%

Infectivity and Morphology of Bovine Coronavirus Inactivated In Vitro by Cationic Photosensitizers

Zhukhovitsky

Shevlyagina²,

Zubasheva³

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Irradiation of PS solutions and sensitized aqueous dispersions of E. coli was carried out in optical cells with a dimensions of 1 × 1 cm 2 . The surface of the dispersions was uniformly irradiated by the LED light with a power density of 20-30 mW cm −2 and wavelength close to the spectral absorption maximum of studied PS [22]. The control of the light sources was carried out using a programmable timer, which made it possible to study the dependence of the effect on the light dose with a step of 0.3 J cm −2 .…”

Section: Methodsmentioning

confidence: 99%

Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers

Меерович¹,

Akhlyustina²,

Denisov³

et al. 2021

Laser Phys. Lett.

View full text Add to dashboard Cite

The aim of this work is the study of antibacterial photodynamic inactivation (APDI) of Escherichia coli (on the example of a model E. coli K12 TG1), the influence of the charge of photosensitizer (PS) molecules on the efficiency of binding with these Gram-negative bacteria. The results obtained confirm that PSs based on polycationic phthalocyanines and synthetic bacteriochlorins provide effective photodynamic inactivation of E. coli, much higher than monocationic methylene blue and electro-neutral bacteriochlorin derivative. The efficiency of APDI, assessed by the intensity of bioluminescence of model E. coli K12 TG1 bacteria, correlates with the charge of PS molecules and the efficiency of electrostatic binding of PS to bacterial cells.

show abstract

Photodynamic inactivation of bacteria: Why it is not enough to excite a photosensitizer

Meerovich,

Akhlyustina,

Romanishkin

et al. 2023

Photodiagnosis and Photodynamic Therapy

View full text Add to dashboard Cite

Photodynamic Action in Thin Sensitized Layers: Estimating the Utilization of Light Energy

Cited by 5 publications

References 14 publications

Infectivity and Morphology of Bovine Coronavirus Inactivated In Vitro by Cationic Photosensitizers

Infectivity and Morphology of Bovine Coronavirus Inactivated In Vitro by Cationic Photosensitizers

Photodynamic inactivation of Escherichia coli bacteria by cationic photosensitizers

Photodynamic inactivation of bacteria: Why it is not enough to excite a photosensitizer

Contact Info

Product

Resources

About