Photodynamic therapy for the treatment of oral squamous carcinoma—Clinical implications resulting from in vitro research

Olek, Marcin; Kasperski, Jacek; Skaba, Dariusz; Wiench, Rafał; Cieślar, Grzegorz; Kawczyk‐Krupka, Aleksandra

doi:10.1016/j.pdpdt.2019.06.012

Cited by 22 publications

(16 citation statements)

References 89 publications

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“…The CAM was successfully used for the study of various types of human carcinoma cell lines, including the glioblastoma [69,70], oesophageal [71], ovarian [72], cervical [73], breast [74], and colorectal [75] tumour cells. The various carcinoma cell lines that were used on the CAM model for photodynamic therapy research were reviewed by Olek et al [76].…”

Section: Cam Model Application In Transplantations and Tumour Grafting Researchmentioning

confidence: 99%

“…Olek et al reviewed the studies that used the CAM model for the research of photodynamic therapy. The CAM was used for the testing of various photosensitive drugs, their pharmacokinetics and light conditions necessary for the photodynamic reaction, and their ability to treat vascular diseases or engrafted carcinomas of various cell lines [76].…”

Section: Cam Model Application In Microbiology and Other Areasmentioning

confidence: 99%

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Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

Kundeková

Máčajová

Meta

et al. 2021

Biology

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The chorioallantoic membrane model (CAM) of an avian embryo is used as an experimental model in various fields of research, including angiogenesis research and drug testing, xenografting and cancer research, and other scientific and commercial disciplines in microbiology, biochemistry, cosmetics, etc. It is a low-cost, low-maintenance, and well-available in vivo animal model that is non-sentient and can be used as an alternative for other mammal experimental models. It respects the principles of the “3R” rule (Replacement, Reduction, and Refinement)—conditions set out for scientific community providing an essential framework for conducting a more human animal research, which is also in line with constantly raising public awareness of welfare and the ethics related to the use of animal experimental models. In this review, we describe the chorioallantoic membrane of an avian embryo, focusing on its properties and development, its advantages and disadvantages as an experimental model, and the possibilities of its application in various fields of biological research. Since the most common chicken CAM model is already well known and described in many publications, we are particularly focusing on the advantages and application of less known avian species that are used for the CAM model—quail, turkey, and duck.

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Section: Cam Model Application In Transplantations and Tumour Grafting Researchmentioning

confidence: 99%

Section: Cam Model Application In Microbiology and Other Areasmentioning

confidence: 99%

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

Kundeková

Máčajová

Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

Wuertz

Biel

et al. 2022

Laryngoscope Investig Oto

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Objective Methylene blue (MB) is a readily available and affordable substrate that can be used as a photosensitizer for photodynamic therapy (PDT). The objective of this study was to determine if PDT with MB can downregulate matrix metalloproteinases (MMPs) related to oral carcinoma. Methods Cell cultures of oral squamous cell carcinoma (CA‐9‐22), oral leukoplakia (MSK‐Leuk1), and immortalized keratinocytes (Rhek‐1A) were photosensitized with MB and treated with PDT. MMP‐9 gene expression was interrogated via qRT‐PCR. The 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay was used to confirm the efficacy of MB PDT. Results MMP‐9 gene expression was found to be significantly decreased in oral carcinoma, leukoplakia, and immortalized keratinocytes with use of MB PDT. Conclusion This work demonstrates that MB‐mediated PDT can downregulate MMPs which are critical to the invasion and metastasis of oral cancer. These results suggest that MB PDT could be a clinically significant and cost‐effective treatment for oral leukoplakia and carcinoma. Level of Evidence NA

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“…It is successfully used in neoplastic and precancerous lesions of the gastrointestinal tract [ 12 , 13 , 14 ], as well as in dermatological [ 15 ] and urological diseases [ 16 ]. PDT is more often used in oral diseases such as lichen planus [ 17 ] or oral squamous cell carcinoma [ 18 , 19 , 20 ]. Antimicrobial use antimicrobial photodynamic therapy (aPDT) has the advantage of having broad-spectrum activity, including gram-negative bacteria, gram-positive bacteria, yeast, and fungi, with no resistance being produced [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Therapy in Orthodontics: A Literature Review

Olek

Machorowska-Pieniążęk

Stós

et al. 2021

Pharmaceutics

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Treatment of malocclusions using fixed orthodontic appliances makes it difficult for patients to perform hygiene procedures. Insufficient removal of bacterial biofilm can cause enamel demineralization, manifesting by visible white spot lesions or periodontal diseases, such as gingivitis periodontitis or gingival hyperplasia. The classic methods of preventing the above problems include, in addition to proper hygiene, ultrasonic scaling, periodontal debridement, and oral rinses based on chlorhexidine. New alternative methods of reducing plaque around brackets are being developed. There is a growing interest among researchers in the possibility of using photodynamic therapy in orthodontics. A literature search for articles corresponding to the topic of this review was performed using the PubMed and Scopus databases and the following keywords: ‘photodynamic therapy’, ‘orthodontics’, and ‘photosensitizer(s)’. Based on the literature review, two main directions of research can be distinguished: clinical research on the use of photodynamic therapy in the prevention of white spot lesions and periodontal diseases, and ex vivo research using a modified orthodontic adhesive by adding photosensitizers to them. Methylene blue is the most frequently used photosensitizer in clinical trials. The effectiveness of antimicrobial photodynamic therapy is mainly compared to the ultrasonic scaler as a single therapy or as an adjunct to the ultrasonic scaler. In their conclusions, the researchers most often emphasize the effectiveness of antimicrobial photodynamic therapy in reducing microbial levels in patients treated with fixed appliances and the possibility of using it as an alternative to routine procedures aimed at maintaining a healthy periodontium. The authors suggest further research on the use of photodynamic therapy to prove the validity of this method in orthodontics. It should also not be forgotten that proper hygiene is the basis for maintaining oral cavity health, and its neglect is a contraindication to orthodontic treatment.

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Photodynamic therapy for the treatment of oral squamous carcinoma—Clinical implications resulting from in vitro research

Cited by 22 publications

References 89 publications

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

Chorioallantoic Membrane Models of Various Avian Species: Differences and Applications

Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

Photodynamic Therapy in Orthodontics: A Literature Review

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