Photodynamic therapy in the treatment of brain tumours. A feasibility study

Vanaclocha, Vicente; Sureda, Manuel; Azinovic, I.; Rebollo, Joseba; Cañón, Rosa; Sapena, Nieves Sáiz; Cases, Francisco García; Brugarolas, Antonio

doi:10.1016/j.pdpdt.2015.05.007

Cited by 17 publications

(19 citation statements)

References 24 publications

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“…Pcs are considered excellent candidates to be used as PSs in PDT treatment of different types of tumors. Regarding brain tumors, several studies using different PSs were reported with promising results 56,57 , but more studies are necessary to probe the effectiveness of this therapeutic strategy as an adjuvant for the treatment of these tumors. The results presented herein support that both Pcs, ZnPc and TAZnPc, have excellent properties that justify testing them in an animal model of glioblastoma.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Velazquez

Miretti

Baumgartner

et al. 2019

Sci Rep

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Glioblastoma multiforme is considered to be one of the most aggressive types of tumors of the central nervous system, with a poor prognosis and short survival periods of ~ one year. The current protocol for glioblastoma treatment includes the surgical excision of the primary tumor followed by radio and chemotherapy. Photodynamic therapy (PDT) is considered a promising strategy for the treatment of several types of tumors. Phthalocyanines (Pcs) are good photosensitizers (PSs) for PDT because they induce cell death in several cellular models. ZnPc (Zn(II)phthalocyanine) is a well-known Pc, extensively tested in different cells and tumor models, but its evaluation on a glioblastoma model has been poorly studied. Herein, we compare the capacity of ZnPc and one of its derivatives, Zn(II)tetraminephthalocyanine (TAZnPc), to photoinactivate glioblastoma cells (T98G, MO59, LN229 and U87-MG) in culture. We measured the cellular uptake, the toxicity in the dark and the subcellular localization of the different Pcs, as well as the clonogenic capacity of surviving cells after PDT. The mechanism of cell death induced after PDT was determined by measuring caspase 3 activation, DNA fragmentation, phosphatidylserine externalization, mitochondrial morphological changes and loss of mitochondrial membrane potential as well as lysosomal membrane integrity. Overall, ZnPc and TAZnPc present good properties to be used as PSs with photoinactivation capacity on glioblastoma cells.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Velazquez

Miretti

Baumgartner

et al. 2019

Sci Rep

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“…PDT could be easily integrated into standard clinical settings: maximum safe surgical resection using photofluorescence can be followed by PDT and RT to remove residual tumor cells [ 7 , 4 ]. Additionally, PDT can be both applied and repeated at relapse of previously irradiated tumors [ 3 , 8 , 9 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient cell death induction in human glioblastoma cells by photodynamic treatment with Tetrahydroporphyrin-Tetratosylat (THPTS) and ionizing irradiation

et al. 2017

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BackgroundSo far, glioblastomas cannot be cured by standard therapy and have an extremely poor median survival of about 15 months. The photodynamic therapy (PDT) with next generation photosensitizers, reaching a higher therapeutic depth, might offer a new, adjuvant treatment strategy in brain cancer therapy. Here, we investigated the effect of THPTS-PDT combined with ionizing irradiation (IR) on glioblastoma cells in vitro and in vivo.ResultsTHPTS colocalized to mitochondria and was not found in the nucleus. THPTS (2–20 μg/ml)-PDT significantly reduced the proliferation, metabolic activity and clonogenic survival and induced cell death mainly through apoptosis and autophagy. THPTS-PDT combined with IR decreased the clonogenicity significantly compared to single treatments. THPTS (≤ 300 μg/ml) alone showed no dark toxicity. The maximum therapeutic depth of THPTS-PDT in C6 glioblastomas was 13 mm.Materials and MethodsThree human glioblastoma cell lines (U-87 MG, A-172, DBTRG-05MG) were incubated with THPTS (1–300 μg/ml) 3–24 hours before laser treatment (760 nm, 30 J/cm2). THPTS localization and effects on metabolic activity, proliferation, cell death mechanisms and long-term reproductive survival were assessed. IR was conducted on an X-ray unit (0.813 Gy/min). Results were verified in vivo on a subcutaneous C6 glioblastoma model in Wistar rats.ConclusionsThis study demonstrated efficient THPTS-PDT in glioblastoma cells, in vitro and in vivo. The combinatorial effects of THPTS-PDT and IR are of specific clinical interest as enhanced eradication of infiltrating glioblastoma cells in the tumor surrounding tissue might possibly reduce the commonly occurring local relapses.

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“…[11,16,[18][19][20] A combined performance of thermal destruction and photodynamic therapy may be considered, as a highly efficient method of increasing the surgery efficiency in infiltrative intracerebral tumors. [21][22][23][24]…”

Section: Resultsmentioning

confidence: 99%

Innovative laser technologies in brain tumors surgery

Розуменко¹

2018

Photobiology and Photomedicine

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The article presents the results of application of innovative laser technologies in the surgical treatment of brain tumors. The article reviews the problems of a differentiated approach to the choice of the laser source as well as optimization of intraoperative application of laser dissection effects, vaporization, coagulation and thermal destruction of tumor tissue, providing increase of surgery efficiency degree and patients’ quality of life. The results of studies on the development of laser surgery planning method and intraoperative navigation maintenance for brain tumors laser surgery method are presented.

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Photodynamic therapy in the treatment of brain tumours. A feasibility study

Cited by 17 publications

References 24 publications

Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Efficient cell death induction in human glioblastoma cells by photodynamic treatment with Tetrahydroporphyrin-Tetratosylat (THPTS) and ionizing irradiation

Innovative laser technologies in brain tumors surgery

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