Photodynamically induced effects in colon carcinoma cells (WiDr) by endogenous photosensitizers generated by incubation with 5-aminolaevulinic acid

Gederaas, Odrun Arna; Rasch, Monique H.; Berg, Kristian; Lagerberg, Johan W.M.; Dubbelman, T. M. A. R.

doi:10.1016/s1011-1344(99)00051-2

Cited by 25 publications

(15 citation statements)

References 27 publications

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“…Interestingly, this could be in accordance with Henning et al (35) who demonstrated that acid phosphatase has a higher affinity to the lysosomal membrane than ␤-glucoronidase and therefore could be located inside the range of the photodynamic action. By measuring the activity of enzymes located in the cytosol of WiDr cells (glyceraldehyde 3-phosphate dehydrogenase and lactate dehydrogenase) and lysosomes (acid phosphatase and ␤-glu-coronidase) 3.5 h after 5-ALA and light treatment Gederaas et al (36) found that these enzymes were not photodynamically inactivated. However, ALA-PDT induced inactivation of the tetrazolium salt in the 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide assay and reduction of the intracellular ATP level, indicating mitochondria targeting.…”

Section: Discussionmentioning

confidence: 99%

5-Aminolevulinic Acid–based Photochemical Internalization of the Immunotoxin MOC31-gelonin Generates Synergistic Cytotoxic Effects In Vitro¶

Selbo¹,

Kaalhus²,

Sivam³

et al. 2001

Photochem Photobiol

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Photochemical internalization (PCI) is a novel method for the endosomal or lysosomal release of membrane-impermeable molecules into the cytosol of target cells. This novel technology is based on the photodynamically induced rupture of endocytic vesicles preloaded with molecules of therapeutic interest. PCI of the ribosome-inactivating plant toxin gelonin and the immunotoxin monoclonal antibody 31 (MOC31) gelonin has been performed previously by the use of the endocytic vesicle-localizing photosensitizers TPPS2a and AIPcS2a and light, demonstrating synergistic toxicity against the more than 20 different cell lines tested, most of them of neoplastic origin. In this study we demonstrate that 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) is also capable of inducing PCI of MOC31-gelonin in the human colon adenocarcinoma cell line WiDr. The cells were incubated with 1 mM 5-ALA for up to 8 h in serum-free medium and from 24 to 96 h in serum-containing medium. Fluorescence microscopical studies indicate a partial plasma membrane localization of PpIX when 5-ALA was applied under serum-free conditions. This plasma membrane localization was not seen when 5-ALA was given in the presence of serum. There was a granular component of the PpIX localization in addition to a diffuse cytoplasmic localization. The granular component resembled the localization of the fluorescent dye conjugate Alexa-gelonin and the lysosomal localizing dye acridine orange. Our present results provide evidence for an endocytic vesicle-associated fraction of PpIX after 5-ALA incubation of the WiDr cells. We demonstrate that PCI, by combining 5-ALA, MOC31-gelonin and light, induces a synergistic cytotoxic effect against the WiDr cells.

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Section: Discussionmentioning

confidence: 99%

5-Aminolevulinic Acid–based Photochemical Internalization of the Immunotoxin MOC31-gelonin Generates Synergistic Cytotoxic Effects In Vitro¶

Selbo¹,

Kaalhus²,

Sivam³

et al. 2001

Photochem Photobiol

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“…The photosensitizer was mainly located perinuclear. Photosensitization with ALA as a prodrug induces damage to mitochondrial function without inhibiting lysosomal and cytosolic enzymes [41]. Mitsunaga et al investigated the roles of Bax and p53 proteins in photosensitivity of human HCT116 CRC cells by using lysosome-localizing chlorin photosensitizer, ATX-S10Na (II).…”

Section: Ala-mediated Photodynamic Effect Toward Crc Cellsmentioning

confidence: 99%

Photodynamic therapy in colorectal cancer treatment—The state of the art in preclinical research

Kawczyk‐Krupka

Bugaj

Latos

et al. 2016

Photodiagnosis and Photodynamic Therapy

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“…135 Other colon cancer cell culture studies have investigated the use of the photosensitizers ALA, and methylene blue, among others, and their comparative mechanisms of inducing cell death. [136][137][138][139][140][141] The science of PDT has tried to keep pace with the enthusiasm of clinicians for treating patients. In 1987, Barr et al demonstrated the effect of aluminum sulfonated phthalocyanine PDT on normal rat colon.…”

Section: Pdt For Colorectal Cancermentioning

confidence: 99%

Uses of Photodynamic Therapy in Premalignant and Malignant Lesions of the Gastrointestinal Tract Beyond the Esophagus

Wolfsen¹

2005

Journal of Clinical Gastroenterology

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Much has recently been written regarding the use of photodynamic therapy for the treatment of esophageal carcinoma and dysplastic Barrett's esophagus. This review, however, describes the clinical experience using photodynamic therapy with various photosensitizer agents for the treatment of diseases in other areas of the gut, especially the pancreaticobiliary tract where European studies have established the role of porfimer sodium photodynamic therapy in the management of patients with cholangiocarcinoma.

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Photodynamically induced effects in colon carcinoma cells (WiDr) by endogenous photosensitizers generated by incubation with 5-aminolaevulinic acid

Cited by 25 publications

References 27 publications

5-Aminolevulinic Acid–based Photochemical Internalization of the Immunotoxin MOC31-gelonin Generates Synergistic Cytotoxic Effects In Vitro¶

5-Aminolevulinic Acid–based Photochemical Internalization of the Immunotoxin MOC31-gelonin Generates Synergistic Cytotoxic Effects In Vitro¶

Photodynamic therapy in colorectal cancer treatment—The state of the art in preclinical research

Uses of Photodynamic Therapy in Premalignant and Malignant Lesions of the Gastrointestinal Tract Beyond the Esophagus

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