Photoenzymatic Activity of Artificial–Natural Bienzyme Applied in Biodegradation of Methylene Blue and Accelerating Polymerization of Dopamine

Abstract

Enzymes as biocatalysts have attracted extensive attention. In addition to immobilizing or encapsulating various enzymes for combating the easy loss of enzymatic activity, strengthening the enzymatic activity upon light irradiation is a challenge. To the best of our knowledge, the work of spatiotemporally modulating the catalytic activity of artificialnatural bienzymes with a near-infrared light irradiation has not been reported. Inspired by immobilized enzymes and nanozymes, herein a platinum nanozyme was synthesized; subsequently, the platinum nanozyme was grafted on the body of laccase, thus successfully obtaining the artificial-natural bienzyme. The threedimensional structure of the artificial-natural bienzyme was greatly different from that of the immobilized enzyme or the encapsulated enzyme. The platinum nanozyme possessed excellent laccase-like activity, which was 3.7 times higher than that of laccase. Meanwhile, the coordination between the platinum nanozyme and laccase was proved. Besides, the cascaded catalysis of artificialnatural bienzyme was verified with hydrogen peroxide as a mediator. The enzymatic activities of artificial-natural bienzyme with and without near-infrared light irradiation were, respectively, 46.2 and 29.5% higher than that of free laccase. Moreover, the reversible catalytic activity of the coupled enzyme could be manipulated with and without a near-infrared light at 808 nm. As a result, the degradation rates of methylene blue catalyzed by the coupled enzyme and the platinum nanozyme were higher than that of laccase. Furthermore, accelerating polymerization of the dopamine was also demonstrated. Briefly, this facile strategy may provide a universal approach to control the catalytic activity of other natural enzymes.

Hybrid Enzymatic/Photocatalytic Degradation of Antibiotics via Morphologically Programmable Light‐Driven ZnO Microrobots

“…In addition, the Michaelis–Menten constant ( K m ) and the maximum velocity ( V max ) were determined according to the Lineweaver–Burk plot (Figure S8, Supporting Information). [ 7,37 ] For the immobilized laccase, these calculations show K m and V max as 0.249 mM and 0.074 µmol min −1 , respectively.…”

Multifunctional composite coatings with hydrophobic, UV-resistant, anti-oxidative, and photothermal performance for healthcare

“…However, it also has lower catalytic activity, limiting its widely commercial application. Herein, the platinum nanozyme with the size ∼2.0 nm was obtained by our previous work [27] , and it can rapidly catalyze the degradation of methylene blue and the oxidation of dopamine in contrast to the natural laccase. Thus, the platinum nanozyme was selected as a high-performance catalyst for fabricating the multifunctional composite coatings.…”

“…Chitosan (CS, 80-95% degree of deacetylation), anhydrous citric acid (99.5%), sodium monohydrogen phosphate dodecahydrate (AR), hydrogen peroxide (AR), anhydrous acetic acid (AR), ammonia (AR), indigo (CP), silica dioxide powder (AR), and sodium dodecyl benzene sulfonate (SDBS, AR) were supplied by Sinopharm Chemical Reagent Co. Ltd. The platinum nanozyme solution obtained by our previous work [27] . All chemicals were used as received.…”

Study on the photocatalytic properties differences between the 1-D and 3-D W18O49 particles

“…To better solve the above problems, more and more approaches have been carried out to degrade organic dyes, such as physical absorption, solvent extraction, biodegradation, and photocatalytic oxidation. [21][22][23][24][25][26] Among these methods, photocatalytic degradation, which utilizes semiconductor and light to solve organic dyes, is undoubtedly the most promising one by virtue of its environmental protection, high efficiency and advanced oxidation in sewage disposal. [27][28][29] Having said all of the foregoing, it is very necessary to use tungsten oxide material with many superior photocatalytic performance advantages to photodegrade organic dyes.…”