Photograftable Zwitterionic Coatings Prevent Staphylococcus aureus and Staphylococcus epidermidis Adhesion to PDMS Surfaces

Abstract

Due to its attractive mechanical properties and biocompatibility, poly­(dimethyl)­siloxane (PDMS) is widely used in the fabrication of biomedical materials. On the other hand, PDMS is also prone to adsorption of both proteins and bacteria, making PDMS implants susceptible to infection. Herein, we examine the use of durably cross-linked zwitterionic coatings for PDMS surfaces to mitigate bacterial adhesion. Using a single-step photografting technique, poly­(sulfobetaine methacrylate) (pSBMA) and poly­(carboxybetaine methacrylate) (pCBMA) thin films were covalently attached to PDMS substrates. The abilities of these coatings to resist the adhesion of Staphylococcus aureus and Staphylococcus epidermidis were tested in vitro under both wet and droplet conditions, as well as in subcutaneous and transcutaneous implantation models using Sprague-Dawley rats. Zwitterionic thin films effectively reduced bacterial adhesion in both in vitro and in vivo conditions. This was particularly true for pCBMA-coated materials, which exhibited significant reduction in bacterial adhesion and growth with respect to S. aureus and S. epidermidis for all in vitro conditions as well as the ability to resist bacterial growth on PDMS implants. The results of this study suggest that a simple and durable photografting process can be used to produce polymer thin films capable of preventing infection of implantable medical devices.

UV-Assisted Deposition of Antibacterial Ag–Tannic Acid Nanocomposite Coating

“…Polydimethylsiloxane (PDMS) has been widely used in contact lens, catheters, pacemaker leads, and other biomedical devices. , Unfortunately, like many other hydrophobic materials, the native PDMS surface is always susceptible to uncontrolled bacterial contamination . Many efforts have been made to reduce bacterial adhesion on the PDMS substrates through surface modification. , In this study, the UV-assisted one-step deposition of TA and Ag NPs was employed to functionalize the PDMS surface. The deposition of TA and Ag NPs in the absence of UV irradiation was compared.…”

“…34 Many efforts have been made to reduce bacterial adhesion on the PDMS substrates through surface modification. 35,36 In this study, the UV-assisted one-step deposition of TA and Ag NPs was employed to functionalize the PDMS surface. The deposition of TA and Ag NPs in the absence of UV irradiation was compared.…”

Imprinted Polydimethylsiloxane-Graphene Oxide Composite Receptor for the Biomimetic Thermal Sensing of Escherichia coli

“…As cell recognition in surface imprinted polymers is determined predominantly by chemical interactions in addition to shape complementarity, it is possible to tune the selectivity of the system by introducing simple modifications to PDMS, − in order to decrease or increase nonspecific interactions with other bacteria (e.g., hydrophobic or electrostatic).…”

Cochlear implant material effects on inflammatory cell function and foreign body response

“…This information can potentially be used to influence the design of future generations of neural prostheses. For example, thin film coatings of CI electrode arrays with ultra-low fouling biomaterials have the potential to dramatically reduce cellular adhesion to the electrode array surface (Leigh et al, 2017;Leigh et al, 2019;Shen et al, 2021). Another strategy to mitigate the cochlear tissue response to implanted electrode arrays is to delivery pharmaceuticals that modulate cellular responses.…”