Photoinactivation in vivo of superoxide dismutase and catalase in the cyanobacterium Microcystis aeruginosa

Tytler, Ewan M.

doi:10.1016/0378-1097(84)90216-7

FEMS Microbiology Letters

1984

DOI: 10.1016/0378-1097(84)90216-7

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Photoinactivation in vivo of superoxide dismutase and catalase in the cyanobacterium Microcystis aeruginosa

Ewan M. Tytler

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1986

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Cited by 10 publications

(11 citation statements)

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“…This is consistent with previous reports indicating that catalase is itself photosensitive (Tytler et al. , Morris et al. ).…”

supporting

confidence: 94%

“…However, the degree of attenuation and night-time consumption decreased on day 2 relative to day 1, suggesting a loss of catalase activity over the course of the experiment. This is consistent with previous reports indicating that catalase is itself photosensitive (Tytler et al 1984, Morris et al 2011. Nevertheless, it appears that 0.1-1 unit Á mL À1 of catalase may be used in conjunction with HEPES to simulate the HOOH periodicity observed in natural waters, at least for the first 1 or 2 d.…”

supporting

confidence: 91%

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Continuous hydrogen peroxide production by organic buffers in phytoplankton culture media

Morris

Zinser

2013

Journal of Phycology

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We investigated the production of hydrogen peroxide (HOOH) in illuminated seawater media containing a variety of zwitterionic buffers. Production rates varied extensively among buffers, with 4-(2-hydroxyethyl)1-piperazineethanesulfonic acid (HEPES) highest and N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS) among the lowest. The rate of HOOH accumulation was remarkably consistent over many days, and increased linearly with buffer concentration, natural seawater concentration, and light level. Concentrations of HEPES commonly used in culture media (1-10 mM) generated enough HOOH to kill the axenic Prochlorococcus strain VOL1 during growth in enriched seawater media at lower, environmentally realistic cell concentrations and/or under high light exposure. We also demonstrated that HEPES can be used experimentally to study the biological effects of chronic exposure to sublethal levels of HOOH such as may be experienced by light-exposed microorganisms.

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“…This is consistent with previous reports indicating that catalase is itself photosensitive (Tytler et al. , Morris et al. ).…”

supporting

confidence: 94%

supporting

confidence: 91%

Continuous hydrogen peroxide production by organic buffers in phytoplankton culture media

Morris

Zinser

2013

Journal of Phycology

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“…These results thus suggest that the elimination of extracellular HOOH by the periplasmic catalase of V. fischeri is a necessary component of this organism's helping phenotype. Direct tests for the sufficiency of purified catalase to help Prochlorococcus grow were complicated by the fact that catalase is vulnerable to photoinactivation (6,13,25). This loss of activity is likely very significant given the long incubation periods in the light that are required for growth on solid medium (1 to 2 months for colonies).…”

mentioning

confidence: 99%

Facilitation of Robust Growth of Prochlorococcus Colonies and Dilute Liquid Cultures by “Helper” Heterotrophic Bacteria

Morris

Kirkegaard

Szul

et al. 2008

Appl Environ Microbiol

274

308

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Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacteria. Once the Prochlorococcus cell concentration surpasses a critical threshold, the "helper" heterotrophs can be eliminated with antibiotics to produce axenic cultures. Our preliminary evidence suggests that one mechanism by which the heterotrophs help Prochlorococcus is the reduction of oxidative stress.Members of the genus Prochlorococcus are the most abundant marine photosynthetic organisms and, as such, are major contributors to photosynthesis in the ocean (20). Over 30 strains of Prochlorococcus have been brought into culture, isolated from many locations within the band from 40°N to 40°S, including the North Atlantic, the North and South Pacific Oceans, the Mediterranean Sea, and the Arabian Sea (20). Despite this success, very few pure cultures of Prochlorococcus (e.g., those of strains PCC 9511 and MIT 9313 [18,22]) have been obtained. The vast majority of cultures contain heterotrophic microbes as contaminants; these heterotrophs were cocultured from the marine environment during the isolation procedure, which has relied thus far exclusively on liquid cultivation. While plating for contiguous lawns of Prochlorococcus has proven to be productive (15), attempts at colony formation (by pour plating or surface streak plating) have thus far met with significantly less success. Recovery efficiencies of the pour plating technique of 0.1 to 10% have been reported previously for some strains (15,24), but this technique has yet to produce pure cultures (15). The inability to readily obtain clonal, pure cultures of Prochlorococcus has severely limited progress in the genetic and physiological analysis of this ecologically important lineage.The "helper" phenotype of heterotrophic bacteria. Standard dilution streaking of contaminated Prochlorococcus cultures onto semisolid medium failed to produce axenic colonies. Colonies formed only within a visible mass of the contaminant heterotrophic bacteria; such masses appeared typically at the sites of the earliest, heaviest dilution streaks (data not shown). One interpretation of these results was that Prochlorococcus was able to grow only in the presence of the contaminating bacteria, perhaps because the bacteria provide a growth factor and/or remove an inhibitory factor. Coculturing with heterotrophic bacteria is required for the growth of some bacterial isolates (9) and is known to improve the growth of dinoflagellates (2, 7), suggesting that a similar interaction may help Prochlorococcus. To test this hypothesis, a heterotrophic contaminant (designated EZ55) of a culture of the ...

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“…Photoinactivation of catalase activity by visible light was demonstrated to occur also in intact cells or tissues, such as hepatocytes (5), cyanobacteria (26,27), and leaves (6)(7)(8). The heme group of the enzyme (4) and, in green leaves, Chls as well (7) act as photoreceptors mediating catalase inactivation.…”

mentioning

confidence: 99%

Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

Hertwig

Streb²,

Feierabend³

1992

Plant Physiol.

213

136

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The enzyme catalase (EC The enzyme catalase from both animal and plant sources is light sensitive (2,4,7,23 we investigated the dynamics of the catalase polypeptide with labeling and chase experiments and immunochemical techniques; for comparison, we investigated the dynamics of the Dl protein in rye leaves exposed to different photon flux densities.The maintenance of proteins depending on rapid tumover will generally be sensitive to all environmental conditions that either overload or hamper the capacity of repair synthesis, as previously described for low temperature (29). Also, other stress conditions known to affect protein synthesis, such as salt (13, 16) or heat shock (28), can be expected to induce specific declines in catalase and possibly in the Dl protein even in only moderate light, simply by preventing repair. Therefore, their influence on catalase and on Dl was investigated. MATERIALS AND METHODS Plant Material and Growing ConditionsExperiments were performed with leaf sections of 6-d-old rye seedlings (Secale cereale L. cv Halo). Seeds were surface sterilized by a 10-min vacuum infiltration and about 30-min soaking in a freshly prepared, filtered solution of 3% (w/v) calcium hypochlorite chloride, thoroughly washed with demineralized H20, and grown at 220C in glass-covered plastic boxes on filter paper (Schleicher & Schull; No. 598

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Photoinactivation in vivo of superoxide dismutase and catalase in the cyanobacterium Microcystis aeruginosa

Cited by 10 publications

References 12 publications

Continuous hydrogen peroxide production by organic buffers in phytoplankton culture media

Continuous hydrogen peroxide production by organic buffers in phytoplankton culture media

Facilitation of Robust Growth of Prochlorococcus Colonies and Dilute Liquid Cultures by “Helper” Heterotrophic Bacteria

Light Dependence of Catalase Synthesis and Degradation in Leaves and the Influence of Interfering Stress Conditions

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