Photoluminescence label-free immunosensor for the detection of Aflatoxin B1 using polyacrylonitrile/zinc oxide nanofibers

Myndrul, Valerii; Coy, Emerson; Bechelany, Mikhael; Iatsunskyi, Igor

doi:10.1016/j.msec.2020.111401

Cited by 59 publications

(44 citation statements)

References 69 publications

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“…[ 2 , 3 ]. Moreover, due to its good biocompatibility, low cytotoxicity, high surface to volume ratio with enhanced surface reactivity, and antistatic, antimicrobial, antibacterial, and antifungal properties, ZnO found broad application in biomedicine as a drug carrier, a biomarker for cell labelling, a biosensor, and an antibacterial agent [ 4 , 5 , 6 ]. The material’s functional properties can be achieved by modification of their crystal structure, e.g., by incorporation of impurities into the crystal structure or by surface modification/construction of the materials with the core-shell structure, as well as by obtaining organic/inorganic or inorganic/inorganic composites [ 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Gelatin-ZnO Nanofibers for Antibacterial Applications

et al. 2020

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In this study, GNF@ZnO composites (gelatin nanofibers (GNF) with zinc oxide (ZnO) nanoparticles (NPs)) as a novel antibacterial agent were obtained using a wet chemistry approach. The physicochemical characterization of ZnO nanoparticles (NPs) and GNF@ZnO composites, as well as the evaluation of their antibacterial activity toward Gram-positive (Staphyloccocus aureus and Bacillus pumilus) and Gram-negative (Escherichia coli and Pseudomonas fluorescens) bacteria were performed. ZnO NPs were synthesized using a facile sol-gel approach. Gelatin nanofibers (GNF) were obtained by an electrospinning technique. GNF@ZnO composites were obtained by adding previously produced GNF into a Zn2+ methanol solution during ZnO NPs synthesis. Crystal structure, phase, and elemental compositions, morphology, as well as photoluminescent properties of pristine ZnO NPs, pristine GNF, and GNF@ZnO composites were characterized using powder X-ray diffraction (XRD), FTIR analysis, transmission and scanning electron microscopies (TEM/SEM), and photoluminescence spectroscopy. SEM, EDX, as well as FTIR analyses, confirmed the adsorption of ZnO NPs on the GNF surface. The pristine ZnO NPs were highly crystalline and monodispersed with a size of approximately 7 nm and had a high surface area (83 m2/g). The thickness of the pristine gelatin nanofiber was around 1 µm. The antibacterial properties of GNF@ZnO composites were investigated by a disk diffusion assay on agar plates. Results show that both pristine ZnO NPs and their GNF-based composites have the strongest antibacterial properties against Pseudomonas fluorescence and Staphylococcus aureus, with the zone of inhibition above 10 mm. Right behind them is Escherichia coli with slightly less inhibition of bacterial growth. These properties of GNF@ZnO composites suggest their suitability for a range of antimicrobial uses, such as in the food industry or in biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Gelatin-ZnO Nanofibers for Antibacterial Applications

et al. 2020

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“…ZnO is an oxide semiconductor that has been used to produce immunosensors. One example of the functionalization strategies of this oxide involves its silanization with APTES and subsequent activation with GA. After that process, Protein A can be deposited over the surface of the sensor, followed by the antibody immobilization and surface passivation with BSA [ 115 , 116 ].…”

Section: Biofunctionalization Strategies For Optical Fiber Immunosensorsmentioning

confidence: 99%

“…A plethora of other optical fiber-based immunosensors have been reported in recent years for different applications, including detection of mycotoxins relevant for food safety [ 115 , 116 , 141 ] and BSA [ 113 , 142 ], among others. In Table 2 , a summary of the immunosensors described is presented, highlighting the main figures of merit.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Immunosensing Based on Optical Fiber Technology: Recent Advances

et al. 2021

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The evolution of optical fiber technology has revolutionized a variety of fields, from optical transmission to environmental monitoring and biomedicine, given their unique properties and versatility. For biosensing purposes, the light guided in the fiber core is exposed to the surrounding media where the analytes of interest are detected by different techniques, according to the optical fiber configuration and biofunctionalization strategy employed. These configurations differ in manufacturing complexity, cost and overall performance. The biofunctionalization strategies can be carried out directly on bare fibers or on coated fibers. The former relies on interactions between the evanescent wave (EW) of the fiber and the analyte of interest, whereas the latter can comprise plasmonic methods such as surface plasmon resonance (SPR) and localized SPR (LSPR), both originating from the interaction between light and metal surface electrons. This review presents the basics of optical fiber immunosensors for a broad audience as well as the more recent research trends on the topic. Several optical fiber configurations used for biosensing applications are highlighted, namely uncladded, U-shape, D-shape, tapered, end-face reflected, fiber gratings and special optical fibers, alongside practical application examples. Furthermore, EW, SPR, LSPR and biofunctionalization strategies, as well as the most recent advances and applications of immunosensors, are also covered. Finally, the main challenges and an outlook over the future direction of the field is presented.

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“…In an attempt to early diagnose UTIs, a portable bacteria-grabbing nanochip based on surface-enhanced Raman scattering (SERS) was generated by Yang et al [99] to detect three species of uropathogens (Proteus mirabilis PRM1, Escherichia coli CFT 073, Pseudo- As mentioned above, the existing approaches of urinalysis are complex and timeconsuming and they lack both accuracy and precision [95]. Due to their fast response time and improved precision and accuracy, photoluminescence (PL)-based biosensors attracted more attention in recent years [95][96][97][98]. Concerning this, Vasudevan et al [95] developed a nanosensor based on cysteamine-attached ZnO NPs (ZnO-Cys) for the identification of N-acyl-homoserine lactones (AHLs) Gram-negative bacteria.…”

Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

et al. 2021

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The diagnosis and treatment of urinary tract infections (UTIs) remain challenging due to the lack of convenient assessment techniques and to the resistance to conventional antimicrobial therapy, showing the need for novel approaches to address such problems. In this regard, nanotechnology has a strong potential for both the diagnosis and therapy of UTIs via controlled delivery of antimicrobials upon stable, effective and sustained drug release. On one side, nanoscience allowed the production of various nanomaterial-based evaluation tools as precise, effective, and rapid procedures for the identification of UTIs. On the other side, nanotechnology brought tremendous breakthroughs for the treatment of UTIs based on the use of metallic nanoparticles (NPs) for instance, owing to the antimicrobial properties of metals, or of surface-tailored nanocarriers, allowing to overcome multidrug-resistance and prevent biofilm formation via targeted drug delivery to desired sites of action and preventing the development of cytotoxic processes in healthy cells. The goal of the current study is therefore to present the newest developments for the diagnosis and treatment of UTIs based on nanotechnology procedures in relation to the currently available techniques.

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Photoluminescence label-free immunosensor for the detection of Aflatoxin B1 using polyacrylonitrile/zinc oxide nanofibers

Cited by 59 publications

References 69 publications

Fabrication of Gelatin-ZnO Nanofibers for Antibacterial Applications

Fabrication of Gelatin-ZnO Nanofibers for Antibacterial Applications

Immunosensing Based on Optical Fiber Technology: Recent Advances

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

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