Photometric assay of methanol and formaldehyde in industrial waste-waters using alcohol oxidase and 3-methyl-2-benzothiazolinone hydrazone

Alcohol oxidase- and formaldehyde dehydrogenase-based enzymatic methods for formaldehyde assay in fish food products

“…We have tested a possibility to use AOX for enzymatic assay of FA in wastes (Gonchar et al, 1998) and even in the presence of natural substrate of AOX, methanol (Sibirny et al, 2008). This enzyme was also used for construction of amperometric biosensor that is able to measure FA in the absence of alcohols (Dmytruk et al, 2007).…”

“…Recent achievements in the field of FA analysis include enzymatic and biosensor approaches based on the use of bacterial or yeast recombinant formaldehyde dehydrogenase (Ali et al, 2006;Ali et al, 2007;Demkiv, Paryzhak, Gayda, Sibirny, & Gonchar, 2007;Demkiv et al, 2008;Ho & Richards, 1990;Nikitina et al, 2007;Paryzhak, Demkiv, Schuhmann, & Gonchar, 2008) and yeast alcohol oxidase which is able to oxidise FA (Dmytruk et al, 2007;Gonchar, Strzelczyk, Maidan, Bień , & Sibirny, 1998;Sibirny et al, 2008).…”

Spectrophotometric Estimation of Gemcitabine HCl in Pharmaceutical Dosage form via Oxidative Coupling Reaction

“…and heterocyclic bases 5 , MBTH (3-Methyl 1, 2-Benzothiazolinehy-drazone hydrochloride) is also used in the analysis of several compounds of biochemical 6 , pharmaceutical 7 and insecticidal 8 . MBTH responds to the enzymatic activity of some of the enzymes like peroxidase 9 , lactase 10 alcohol oxidase 11 and toluene-4-monooxygenase 12 in the presence of corresponding substrates. The present study documents an accurate, sensitive, rapid, selective and reproducible visible spectrophotometric assay which meets an accepted analytical validation.…”

Formaldehyde Oxidizing Enzymes and Genetically Modified Yeast Hansenula polymorpha Cells in Monitoring and Removal of Formaldehyde

“…Lower levels of FA in anti-diphtheria vaccines, obtained using the enzymatic method as compared to the chemical methods may be due to the inhibitory effect on the enzyme by the Hg-containing compound, 0.01% merthiolate, a vaccine preservative. (Sibirny et al, 2008). Pre-existing FA content is detected without treating samples by AOX (CD 0 in reaction 9); and methanol content is determined by an increase in colored product concentration due to the methanol-oxidising reaction (CD Δ in reaction 10).…”

“…As a chromogen, 3,3',5,5'-tetramethyl-benzidine dihydrochloride (TMB) was used. The principle of FA determination by AOP-method is based on the measurement of the dyeproduct accumulation in peroxidative oxidation of chromogen by H 2 O 2 (Sibirny et al, 2008) generated from FA in AOX reaction (see reactions 5, 7):…”