Photon absorption in the mitochondria: Potential immediate and early events associated with photobiomodulation

Denton, Michael L.; Gonzalez, Cherry C.; Noojin, Gary D.; Rickman, John M.; O’Connor, Sean P.; Lalonde, Joshua W.; Yakovlev, Vladislav V.; Pope, Nathaniel J.

doi:10.1117/12.2508518

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Experimental evidence [Karu, 2008; De Freitas and Hamblin, 2016; Denton et al, 2019] shows that very important reactions take place inside mitochondria due to PBM [Scheffler, 2008]. The same was proven by experiments involving mitochondria in vitro [Passarella and Karu, 2014].…”

Section: Introductionmentioning

confidence: 94%

“…Experimental evidence [Karu, 2008;De Freitas and Hamblin, 2016;Denton et al, 2019] shows that -----------------------------------------------------------------------------------------This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. very important reactions take place inside mitochondria due to PBM [Scheffler, 2008].…”

Section: Introductionmentioning

confidence: 99%

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Electromagnetic Dosimetry for Isolated Mitochondria Exposed to Near‐Infrared Continuous‐Wave Illumination in Photobiomodulation Experiments

Amaroli

Benedicenti

Bianco

et al. 2021

Bioelectromagnetics

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This paper presents results on the electromagnetic field computed inside isolated mitochondria when they are exposed to near-infrared illuminations with reference to photobiomodulation experiments. The accurate calculation of the electromagnetic dose is considered to be important for a better understanding of the mechanism of interaction of light with these organelles and to improve the reliability and repeatability of the experiments. To get such results, we introduce several models. Even though they refer to a well-defined experimental setup, different models are necessary to take into account the possible different dispositions of the mitochondria, and of the differences in their dimensions and in their constitutive parameters. Different wavelengths and polarizations are considered as well. The effects of all parameters on the electromagnetic field inside mitochondria are discussed. Bioelectromagnetics. 2021;42:384-397.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Electromagnetic Dosimetry for Isolated Mitochondria Exposed to Near‐Infrared Continuous‐Wave Illumination in Photobiomodulation Experiments

Amaroli

Benedicenti

Bianco

et al. 2021

Bioelectromagnetics

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Implantable and transcutaneous photobiomodulation promote neuroregeneration and recovery of lost function after spinal cord injury

Stevens,

Hadis,

Phillips

et al. 2024

Bioengineering & Transla Med

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Spinal cord injury (SCI) is a cause of profound and irreversible damage, with no effective therapy to promote functional recovery. Photobiomodulation (PBM) may provide a viable therapeutic approach using red or near‐infrared light to promote recovery after SCI by mitigating neuroinflammation and preventing neuronal apoptosis. Our current study aimed to optimize PBM dose regimens and develop and validate the efficacy of an invasive PBM delivery paradigm for SCI. Dose optimization studies were performed using a serum withdrawal model of injury in cultures of primary adult rat dorsal root ganglion neurons (DRGN). Implantable and transcutaneous PBM delivery protocols were developed and validated using cadaveric modeling. The efficacy of PBM in promoting recovery after SCI in vivo was studied in a dorsal column crush injury model of SCI in adult rats. Optimal neuroprotection in vitro was achieved between 4 and 22 mW/cm2. 11 mW/cm2 for 1 min per day (0.66 J/cm2) increased cell viability by 45% over 5 days (p <0.0001), increasing neurite outgrowth by 25% (p <0.01). A method for invasive application of PBM was developed using a diffusion‐tipped optogenetics fiber optic. Delivery methods for PBM were developed and validated for both invasive (iPBM) and noninvasive (transcutaneous) (tcPBM) application. iPBM and tcPBM (24 mW/cm2 at spinal cord, 1 min per day (1.44 J/cm2) up to 7 days) increased activation of regeneration‐associated protein at 3 days after SCI, increasing GAP43+ axons in DRGN from 18.0% (control) to 41.4% ± 10.5 (iPBM) and 45.8% ± 3.4 (tcPBM) (p <0.05). This corresponded to significant improvements at 6 weeks post‐injury in functional locomotor and sensory function recovery (p <0.01), axonal regeneration (p <0.01), and reduced lesion size (p <0.01). Our results demonstrated that PBM achieved a significant therapeutic benefit after SCI, either using iPBM or tcPBM application and can potentially be developed for clinical use in SCI patients.

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Photon absorption in the mitochondria: Potential immediate and early events associated with photobiomodulation

Cited by 2 publications

References 16 publications

Electromagnetic Dosimetry for Isolated Mitochondria Exposed to Near‐Infrared Continuous‐Wave Illumination in Photobiomodulation Experiments

Electromagnetic Dosimetry for Isolated Mitochondria Exposed to Near‐Infrared Continuous‐Wave Illumination in Photobiomodulation Experiments

Implantable and transcutaneous photobiomodulation promote neuroregeneration and recovery of lost function after spinal cord injury

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