Photon correlation spectroscopy investigations of proteins

Gun'ko, V.М.; Klyueva, Alla V.; Levchuk, Yuri N.; Лебода, Р.

doi:10.1016/s0001-8686(03)00091-5

Cited by 79 publications

(55 citation statements)

References 297 publications

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“…DLS not only provides a route to measure particle size distributions, but also protein (and other biomolecule) molecular weight distributions in solution. 85 PEG is a flexible linear polymer. Consequently, PEG can dramatically influence the Brownian motion of particles by introducing additional frictional drag and thus reducing nanoparticle diffusivity.…”

Section: Dynamic Light Scattering (Dls)mentioning

confidence: 99%

PEGylated gold nanoparticles: polymer quantification as a function of PEG lengths and nanoparticle dimensions

et al. 2013

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Au nanoparticles with diameters ranging between 15 and 170 nm have been synthesised in aqueous solution using a seed-mediated growth method, employing hydroxylamine hydrochloride as a reducing agent. Thiolated polyethylene glycol (mPEG-SH) polymers, with molecular weights ranging from 2100 to 51 000 g mol-1, were used as efficient particle stabilising ligands. Dynamic light scattering and zeta potential measurements confirmed that the overall mean diameter and zeta potential of the capped nanoparticles increased in a non-linear way with increasing molecular weight of the mPEG-SH ligand. Electron microscopy and thermal gravimetric analysis of the polymer-capped nanoparticles, with a mean gold core diameter of 15 nm, revealed that the grafting density of the mPEG-SH ligands decreased from 3.93 to 0.31 PEG nm-2 as the molecular weight of the ligands increased from 2100 to 51 400 g mol-1 respectively, due to increased steric hindrance and polymer conformational entropy with increase in the PEG chain length. Additionally, the number of bound mPEG-SH ligands, with a molecular weight of 10 800 g mol-1, was found to increase in a non-linear way from 278 (σ = 42) to approximately 12 960 PEG (σ = 1227) when the mean Au core diameter increased from 15 to 115 nm respectively. However, the grafting density of mPEG10 000-SH ligands was higher on 15 nm Au nanoparticles and decreased slightly from 1.57 to 0.8 PEG nm-2 when the diameter increased; this effect can be attributed to the fact that smaller particles offer higher surface curvature, therefore allowing increased polymer loading per nm2. Au nanoparticles were also shown to interact with CT-26 cells without causing noticeable toxicity

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Section: Dynamic Light Scattering (Dls)mentioning

confidence: 99%

PEGylated gold nanoparticles: polymer quantification as a function of PEG lengths and nanoparticle dimensions

et al. 2013

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“…Protein molecules can form aggregates [209]. However, these structures must be smaller in size than the collagen macropores; therefore protein diffusion occurred despite the protein pre-runs, but at a lower rate (Fig.…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…The photon correlation spectroscopy (PCS) allows the investigation of mobility of microorganisms [209]. This method could be used to study interactions of mobile (e.g.…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…Notice that the mobility of bacteria was studied using PCS in detail [209,[218][219][220][221][222]. This ability of PCS is of importance in respect of elucidation of the influence of media or cryogel membrane or scaffolds on living microorganisms.…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

“…q is the scalar of the wave scattering vector ( q ) equal to the difference between the final wave vector ( f k ) and the incident ( i k ), n is the refraction index of the liquid,  is the wavelength of the incident light in vacuum, and  is the observation angle of the scattered light [209]. This method was applied to investigate the mobility of Pseudomonas putida [223], Dunaliella viridis [224] and…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

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Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

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287

205

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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Dynamic Light Scattering for Protein Characterization

Schmidt

2010

Encyclopedia of Analytical Chemistry

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Dynamic light scattering (DLS) is an analytical tool for the determination of diffusion coefficients and hydrodynamic sizes of molecules and particles in solution. It allows for the characterization in a size range from the nanometer to lower micrometer scale, which makes it especially valuable in the field of biotechnology. The present article provides an introduction to the theoretical concepts of the DLS technique with emphasis on concepts relevant for protein characterization. Applications for protein analysis are discussed and literature references given. Applications include aggregation detection, crystallization monitoring, and association studies. Specialized applications for particle detection at low scattering angles for protein samples, high‐throughput analysis, and electrophoretic light scattering (ELS) are highlighted.

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Photon correlation spectroscopy investigations of proteins

Cited by 79 publications

References 297 publications

PEGylated gold nanoparticles: polymer quantification as a function of PEG lengths and nanoparticle dimensions

PEGylated gold nanoparticles: polymer quantification as a function of PEG lengths and nanoparticle dimensions

Cryogels: Morphological, structural and adsorption characterisation

Dynamic Light Scattering for Protein Characterization

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