Photoperiod affects the growth and development of yam plantlets obtained by in vitro propagation

Vaillant, Victor; Bade, Pascale; Constant, Camille

doi:10.1007/s10535-005-0007-8

Cited by 27 publications

(20 citation statements)

References 17 publications

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“…Traditionally, D. candidum genetic resources are preserved as whole plants either in field repositories or maintained in greenhouses (Wang and Liu 2006). For both means, preserved plants are subject to risk of loss caused by biological and climatic hazards as well as due to human error by mislabeling samples (Vaillant et al 2005). In addition, traditional maintenance of plant material is highly costly (Wu and Tang 2005).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification

Ming-hua

Senrong

2009

Plant Cell Tiss Organ Cult

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Protocorm-like bodies (PLBs) of Dendrobium candidum Wall. ex Lindl., orchid, were successfully cryopreserved using an encapsulation vitrification method. PLBs were precultured in liquid Murashige and Skoog (MS) medium containing 0.2 mg l -1 a-naphthalene acetic acid and 0.5 mg l -1 6-benzyladenine enriched with 0.75 M sucrose, and grown under continuous light (36 lmol m -2 s -1 ) at 25 ± 1°C for 5 days. PLBs were osmoprotected with a mixture of 2 M glycerol and 1 M sucrose for 80 min at 25°C and dripped in a 0.5 M CaCl 2 solution containing 0.5 M sucrose at 25 ± 1°C and left for 15 min to form Ca-alginate beads (about 4 mm in diameter). Then, these were dehydrated with a plant vitrification solution 2 (PVS 2 ) consisting of 30% (w/v) glycerol, 15% (w/v) ethylene glycol, and 15% (w/v) dimethyl sulfoxide in 0.5 M sucrose, pH 5.8, for 150 min at 0°C. Encapsulated and dehydrated PLBs were plunged directly into liquid nitrogen for 1 h. Cryopreserved PLBs were then rapidly re-warmed in a water bath at 40°C for 3 min and then washed with MS medium containing 1.2 M sucrose for three times at 10 min intervals. Within 60 days, plantlets with the cryopreserved PLBs developed normal shoots and roots, and without any observed morphological abnormalities, were obtained. The survival rate of encapsulated-vitrified PLBs was above 85%. Thus, this encapsulation-vitrification method was deemed promising for cryopreservation of PLBs of D. candidum.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification

Ming-hua

Senrong

2009

Plant Cell Tiss Organ Cult

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“…Al estudiar la respuesta en campo de plantas in vitro de D. alata clon 'Caraqueño' en distintos momentos de plantación en campo, Borges et al (2015) determinaron que el mayor desarrollo vegetativo significativo de la planta se alcanzó a los cuatro meses de cultivo (junio-septiembre), debido a que a partir de este momento se inicia el proceso de tuberización (septiembre). En este aspecto, Vaillant et al (2005) informaron que la tuberización en D. alata se produce doce semanas (tres meses) después del inicio de la brotación de las plantas, lo cual ha sido revelado a partir de estudios sobre el efecto del fotoperíodo en el desarrollo vegetativo de este cultivo.…”

Section: Diámetro Del Tallounclassified

Producción de ñame clon Criollo a partir de bulbillos aéreos

et al. 2018

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© 2018 Agronomía Mesoamericana es desarrollada en la Universidad de Costa Rica y se encuentra licenciada con Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Costa Rica. Para más información escríbanos a pccmca@ucr.ac.cr ResumenEl empleo de bulbillos aéreos de plantas de ñame como material de plantación permite el aprovechamiento del 100% de la cosecha de tubérculos para su consumo con un impacto significativo desde el punto de vista agronómico, económico, social y ambiental. El objetivo de este trabajo fue evaluar los principales indicadores morfológicos y agronómicos de ñame clon Criollo a partir de bulbillos aéreos procedentes de plantas in vitro establecidas durante un primer ciclo de cultivo en campo como fuente de semilla a nivel local. La investigación se desarrolló en el Centro de Estudios de Biotecnología Vegetal de la Universidad de Granma y las localidades colindantes "Las Tamaras" y "La Asunción", consideradas como tratamientos, en el periodo comprendido de abril a diciembre de 2014. A los treinta días se determinó el porcentaje de supervivencia y se realizó un análisis morfológico (diámetro del tallo y relación largo/ancho de la hoja), al cabo del primer, cuarto y séptimo mes de establecidas las plantas en condiciones de campo (veinte plantas por tratamiento). El análisis agronómico se efectuó a los nueve meses de efectuada la plantación, donde se determinaron las siguientes variables: número de tubérculos por planta; masa fresca de tubérculos por planta (kg); masa fresca promedio de tubérculo (kg); número y masa fresca promedio (g) de bulbillos aéreos con un 100% de supervivencia. Las plantas de ñame obtenidas a partir de bulbillos aéreos procedentes de plantas in vitro establecidas durante un primer ciclo de cultivo en campo, presentaron un 100% de supervivencia y un desarrollo morfoagronómico óptimo para la producción y recuperación sostenible de este clon.Palabras clave: Dioscorea alata, plantas in vitro, tubérculos, producción sostenible. AbstractThe employment of air bulbils in yam plants, like plantation material, allows using a 100% of the tubers crop for its consumption, the aforementioned implicates a significant agronomic, economic, social, and environmental impact. The aim of this study was to evaluate the main morphological and agronomic indicators of Creole clone yam in vitro plants obtained by using air bulbils during a first cycle field cultivation as a local level seed source. The research was developed in the Biotechnology Studies Center Plant at Granma University in collaboration with the localities of "Las Tamaras" and "La Asunción" between April and December, 2014. At thirty days, the survival percentage

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“…Many research studies (Economou and Reed 1987) have shown that its variation can influence the growth of the cultures to a greater or lesser degree depending on the genotype: some species benefit from longer photoperiods, others from shorter photoperiods and still others appear to be indifferent. Photoperiod has also shown to influence plant growth during acclimatization stage (Vaillant et al 2005).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks

Morini¹,

Perrone²

2006

Biologia plant.

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Experiments were carried out to evaluate the effects of 4/2 light-dark cycles (4 h of light followed by 2 h of dark) on the rooting responses of shoots cultivated in vitro of the fruit tree rootstocks GF 677 (peach × almond hybrid), Mr.S. 2/5 (Prunus cerasifera), MM 106 (apple Nothern Spy × Paradise M1) and BA 29 (Cydonia oblonga). Under this light regime rooting percentage of GF 677, Mr.S. 2/5 and MM 106 shoots reached 100 % as in the control treatment (16/8), while in BA 29 shoots, short light-dark cycles increased rooting response by about 65 %. Moreover, the shoots of all rootstocks submitted to the 4/2 cycle showed an appreciable increase in root number and length, and an earlier root emergence of about 4 -5 d compared to the 16/8 cycle. Finally, rooting percentage of BA 29 shoots submitted to the 4/2 light regime and treated with 0.2 mg dm -3 indolebutyric acid (IBA), was equal to that reported with 0.4 mg dm -3 IBA under the 16/8 regime, indicating that the former light regime also amplified the rhizogenic effect of auxin.

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Photoperiod affects the growth and development of yam plantlets obtained by in vitro propagation

Cited by 27 publications

References 17 publications

Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification

Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification

Producción de ñame clon Criollo a partir de bulbillos aéreos

Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks

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