Photoperiodic Modulation of Clock Gene Expression in the Avian Premammillary Nucleus

Abstract

The premammillary nucleus (PMM) has been shown to contain a daily endogenous dual-oscillation in dopamine (DA)/melatonin (MEL) as well as c-fos mRNA expression that is associated with the daily photo-inducible phase of gonad growth in turkeys. In the present study, the expression of clock genes (Bmal1, Clock, Cry1, Cry2, Per2 and Per3) in the PMM was determined under short (8 : 16 h light/dark cycle) and long (16 : 8 h light/dark cycle) photoperiods relative to changes associated with the diurnal rhythm of DA and MEL. Constant darkness (0 : 24 h light/dark cycle) was used to assess the endogenous response of clock genes. In addition, light pulses were given at zeitgeber time (ZT) 8, 14 and 20 to ascertain whether clock gene expression is modulated by light pulse stimulation and therefore has a daily phase-related response. In the PMM, the temporal clock gene expression profiles were similar under short and long photoperiods, except that Per3 gene was phase-delayed by approximately 16 h under long photoperiod. In addition, Cry1 and Per3 genes were light-induced at ZT 14, the photosensitive phase for gonad recrudescence, whereas the Clock gene was repressed. Gene expression in established circadian pacemakers, the visual suprachiasmatic nucleus (vSCN) and the pineal, was also determined. Clock genes in the pineal gland were rhythmic under both photoperiods, and were not altered after light pulses at ZT 14, which suggests that pineal clock genes may not be associated with the photosensitive phase and reproductive activities. In the vSCN, clock gene expression was phase-shifted depending on the photoperiod, with apexes at night under short day length and during the day under long day length. Furthermore, light pulses at ZT 14 induced the Per2 gene, whereas it repressed the Bmal1 gene. Taken together, the changes in clock gene expression observed within the PMM were unique compared to the pineal and vSCN, and were induced by long photoperiod and light during the daily photosensitive phase; stimuli that are also documented to promote reproductive activity. These results show that Cry1 and Per3 are involved in the photic response associated with the PMM neuronal activation and are coincident with an essential circadian mechanism (photosensitive phase) controlling the reproductive neuroendocrine system.

Avian circadian organization: A chorus of clocks

“…It is not clear, at this stage, what molecular components link the circadian clock to Dio2 or Dio3. The MBH of quail and the PMM of turkeys rhythmically express clock genes [76,77,92]. In humans, Dio2 is regulated by CCAAT/enhancer-binding proteins, and per2 is known to be a target of these transcription factors as well [61,140].…”

The Ventral Premammillary Nucleus Links Metabolic Cues and Reproduction

“…The PMH of turkeys also presents a distinct circadian expression of clock genes compared to the pineal gland and the brain master clock, the suprachiasmatic nucleus. In particular, Cry1 and Per3 seem to mediate the photic responses associated with the control of the reproductive system (Leclerc et al, 2010). …”

GABAergic Neurotransmission in the Premammillary Nucleus of the Turkey Hypothalamus Regulates Reproductive Seasonality and the Onset of Photorefractoriness

“…The established pathway from the PMM to the pars tuberalis (PT) [15] suggests that the output of the photoperiodic signal is transmitted to the neuroendocrine system cascade that facilitates the release of GnRH-I. Indeed, the photic input for circadian photoentrainment is detected by melanopsin and measured by clock genes localized within DA-Mel neurons in the PMM [15,16]. The encoded photoperiodic information is transmitted via the DAergic system to the PT of the endocrine system as DAergic fibers from PMM DA-Mel neurons project to the PT [15].…”