Photophysical Probes of DNA Sequence‐Directed Structure and Dynamics

Murphy, Catherine J.

doi:10.1002/9780470133576.ch4

Cited by 28 publications

(3 citation statements)

References 335 publications

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“…The emission is so weak that there are no practical uses of intrinsic DNA emission. For this reason a vast array of fluorophores have been developed which bind to DNA and display UV, visible to NIR fluorescence [ [33][34][35][36]. The difficulty with DNA is not that the radiative rates are slow, but that the non-radiative rates are exceedingly fast, so that the bases return to the ground state prior to emission.…”

Section: Effects Of Pmd On Biochemical Fluorophoresmentioning

confidence: 99%

Radiative decay engineering: the role of photonic mode density in biotechnology

Lakowicz

Malicka

Gryczyński

et al. 2003

J. Phys. D: Appl. Phys.

142

165

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Fluorescence detection is a central technology in biological research and biotechnology. A vast array of fluorescent probes are available with diverse spectral properties. These properties were 'engineered' into fluorophores by modification of the chemical structures. Essentially, all present uses of fluorescence rely on the radiation of energy into optically transparent media, the free space which surrounds the fluorophores. In this paper, we summarize an opportunity for novel fluorescence technology based on modification of the photonic mode density around the fluorophore and thus control of its spectral properties. This modification can be accomplished by proximity of fluorophores to metallic particles of gold, silver and possibly others. By engineering the size and shape of the metal particles, and the location of the fluorophores relative to the surfaces, fluorophores can be quenched, display increases in quantum yield, and changes in lifetime. Fluorophore-metal surface combinations can even display directional rather than isotropic emission. We describe recent experimental results and suggest potential biomedical applications of fluorophore-metal particle interactions.

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Section: Effects Of Pmd On Biochemical Fluorophoresmentioning

confidence: 99%

Radiative decay engineering: the role of photonic mode density in biotechnology

Lakowicz

Malicka

Gryczyński

et al. 2003

J. Phys. D: Appl. Phys.

142

165

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show abstract

“…However, the geometry of kinks is not known for all the structural changes in the double helix. In particular, B-A transformations [37][38][39]), binding of the TBP to TATA-box [40,41] and the presence of A-tracts and GGCC-tracts [42][43][44] could cause the formation of kinks holding a more complicated structure than type 1 and type 2 kinks have. For example, TBP induces a strong composite bend over 8 base pairs of TATA-box which includes sharp bends (kinks) by 52 • and 39 • at the first and last base pairs, and smoother bend at 90 • within 6 central base pairs [45,46].…”

Section: Kinks In the Kratky-porod Modelmentioning

confidence: 99%

Strong deformations of DNA: Effect on the persistence length

Simonov

2018

Eur. Phys. J. E

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Extreme deformations of the DNA double helix attracted a lot of attention during the past decades. Particularly, the determination of the persistence length of DNA with extreme local disruptions, or kinks, has become a crucial problem in the studies of many important biological processes. In this paper we review an approach to calculate the persistence length of the double helix by taking into account the formation of kinks of arbitrary configuration. The reviewed approach improves the Kratky-Porod model to determine the type and nature of kinks that occur in the double helix, by measuring a reduction of the persistence length of the kinkable DNA.

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“…It can be used, as anti reflection coatings in silicon based solar cells and infrared windows [9,10] as optical filter since it possesses high transmittance in visible region [11] as sensors [12] lasers [13] planar waveguide [6] etc. Compared to other chalcogenides, ZnS is a very good luminescent material and used as an important phosphor in photoluminescent [14] electroluminescent [15] and cathodoluminescent [16] devices.…”

Section: Introductionmentioning

confidence: 99%