Photophysics of a protein-bound derivative of malachite green that sensitizes the production of singlet oxygen

Dichmann, Lea; Bregnhøj, Mikkel; Westberg, Michael; Poulsen, Thomas B.; Etzerodt, Michael; Ogilby, Peter R.

doi:10.1007/s43630-021-00032-y

Cited by 6 publications

(13 citation statements)

References 67 publications

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“…12), and this yields a O 2 (a 1 Δ g ) lifetime of ˜ 60 µs. Although the noise on the signal precludes an accurate determination, the latter is nevertheless consistent with the expectation for O 2 (a 1 Δ g ) in a D 2 O‐based solution with some quenching by the dissolved protein (51,88) (the lifetime of O 2 (a 1 Δ g ) in neat D 2 O is 67 µs (56)).…”

Section: Resultssupporting

confidence: 80%

“…12), yielding a much longer formation time constant of ˜ 215 µs. A subtle irradiation‐induced change either in the protein and/or in the protein‐encased sensitizer could result in such a large difference in O 2 (a 1 Δ g ) formation time constants (50,59,88). Despite the appreciable change in O 2 (a 1 Δ g ) kinetics seen in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A potential advantage of this approach is that the chromophore would then be exogenous, thus fully exploiting the localization effects of the optogenetic protein (37). The latter approach, however, is not a panacea; the systems available thus far likewise have problems that need resolution (88).…”

Section: The Futurementioning

confidence: 99%

“…Monitoring the kinetics of O 2 (a 1 D g ) formation and removal in time-resolved 1275 nm O 2 (a 1 D g ) ? O 2 (X 3 Σ g -) phosphorescence experiments can likewise provide insight into irradiation-induced changes that may occur with a protein-encased sensitizer (50,59,88). Representative data thus recorded from SOPP3 dissolved in air-saturated D 2 O-based PBS are shown in Fig.…”

Section: Experiments To Help Elucidate the Cause Of Fmn Bleachingmentioning

confidence: 99%

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Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Mogensen

Westberg

Breitenbach

et al. 2021

Photochem & Photobiology

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Protein-encased chromophores that photosensitize the production of reactive oxygen species, ROS, have been the center of recent activity in studies of oxidative stress. One potential attribute of such systems is that the local environment surrounding the chromophore, and that determines the chromophore's photophysics, ideally remains constant and independent of the global environment into which the system is placed. Therefore, a protein-encased sensitizer localized in the mitochondria would arguably have the same photophysics as that protein-encased sensitizer at the plasma membrane, for example. One thus obtains a useful tool to study processes modulated by spatially localized ROS. One ROS of interest is singlet oxygen, O 2 (a 1 D g ). We recently developed a singlet oxygen photosensitizing protein, SOPP, in which flavin mononucleotide, FMN, is encased in a re-engineered lightoxygen-voltage protein. One goal was to ascertain how a version of this system, SOPP3, which selectively makes O 2 (a 1 D g ), in vitro, behaves in a cell. We now demonstrate that SOPP3 undergoes exacerbated irradiation-mediated bleaching when expressed at either the plasma membrane or mitochondria in stable cell lines. We find that the environment around the SOPP3 system affects the bleaching rate, which argues against one of the key suppositions in support of a proteinencased chromophore.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: The Futurementioning

confidence: 99%

Section: Experiments To Help Elucidate the Cause Of Fmn Bleachingmentioning

confidence: 99%

See 2 more Smart Citations

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Mogensen

Westberg

Breitenbach

et al. 2021

Photochem & Photobiology

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“…Many studies have shown that O 2 (a 1 ∆ g ) plays a crucial role in biomedical applications. For instance, it is the main species for the cancer cells inactivation, resulting in its application in cancer and other diseases therapies [10][11][12][13][14]. In addition, O 2 (a 1 ∆ g ) can significantly enhance the combustion of hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

Numerical study of singlet delta oxygen (O₂(a¹Δ_g)) generation and transport in the He/O₂ atmospheric pressure plasma jet

Jiang

Wang

Zhang

et al. 2022

J. Phys. D: Appl. Phys.

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This paper investigates the spatial-temporal evolution and the transport of the single delta oxygen (O2(a1∆g)) of an atmospheric pressure plasma jet using a 2D fluid modeling. The plasma jet is produced in pure helium or helium with small oxygen admixtures (no bigger than 2%), by applying a DC pulse voltage with the peak value of + 4 kV on the annular electrode. It is found that, at the 0.7% O2 admixture, a higher O2(a1∆g) density is obtained inside the tube before the jet impacts the substrate. After the jet propagates along the substrate surface, the peak O2(a1∆g) density is transferred from the tube to the gap. Varying the O2 admixtures percentage in the working gas changes the O2(a1∆g) spatial distribution. The O2(a1∆g) with two thinner edges is dominantly produced in the helium-air mixing layer for pure helium. The addition of O2 in the working gas leads to O2(a1∆g) production in the tube. Meanwhile, the thickness of the O2(a1∆g) edges increases in the helium-air mixing layer. But the radius of the O2(a1∆g) density channel continuously reduces with the O2 admixture. The O2(a1∆g) density and its surface flux present a nonmonotonic behavior, and a peak value is reached at the 0.7% O2 admixture. The influence of the gas flow velocity on the production and transport of O2(a1∆g) is also studied. Increasing the gas flow velocity changes the spatial distribution of the O2(a1∆g) density from the solid structure to the annular structure. At the same time, the lower volume average density and instantaneous flux are obtained at larger gas flow velocity.

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Singlet oxygen quenching by riboflavin

Insińska-Rak,

Henke,

Breitenbach

et al. 2024

Journal of Photochemistry and Photobiology A: Chemistry

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Photophysics of a protein-bound derivative of malachite green that sensitizes the production of singlet oxygen

Cited by 6 publications

References 67 publications

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Numerical study of singlet delta oxygen (O₂(a¹Δ_g)) generation and transport in the He/O₂ atmospheric pressure plasma jet

Singlet oxygen quenching by riboflavin

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Photophysics of a protein-bound derivative of malachite green that sensitizes the production of singlet oxygen

Cited by 6 publications

References 67 publications

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior†

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior†

Numerical study of singlet delta oxygen (O2(a1Δg)) generation and transport in the He/O2 atmospheric pressure plasma jet

Singlet oxygen quenching by riboflavin

Contact Info

Product

Resources

About

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Stable Transfection of the Singlet Oxygen Photosensitizing Protein SOPP3: Examining Aspects of Intracellular Behavior^†

Numerical study of singlet delta oxygen (O₂(a¹Δ_g)) generation and transport in the He/O₂ atmospheric pressure plasma jet