Photoporation and cell transfection using a violet diode laser

Paterson, Lynn; Agate, B.; Comrie, M.; Ferguson, Robert; Lake, T.K.; Morris, James Harry; Carruthers, A. E.; Brown, C.T.A.; Sibbett, W.; Bryant, Peter E.; Gunn‐Moore, Frank; Riches, Andrew; Dholakia, Kishan

doi:10.1364/opex.13.000595

Cited by 78 publications

(47 citation statements)

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“…Depending on the wavelength and pulse duration, the membrane permeation mechanism varies as does the required dose and achievable optoinjection, transfection efficiency and cell viability. In terms of continuous wave lasers, a blue laser can be used for both stable [3] and transient [4] DNA phototransfection, as well as interfering RNA mediated gene silencing [5], though it is currently less mature and proven than the use of short pulse lasers.…”

Section: Biophotonicsmentioning

confidence: 99%

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Antkowiak

Torres-Mapa

Gunn‐Moore

et al. 2010

Journal of Biophotonics

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We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for the controlled and enhanced optoinjection and phototransfection of mammalian cells with a femtosecond light source. The SLM provides full control over the lateral and axial positioning of the beam with sub‐micron precision. Fast beam translation enables time‐sequenced irradiation, which is shown to enhance the optoinjection efficiency and alleviate the problem of exact beam positioning on the cell membrane. We show that irradiation in three axial positions doubles the number of viably optoinjected cells when compared with a single dose. The presented system also enables untargeted raster scan irradiation which provides a higher throughput transfection of adherent cells at the rate of 1 cell per second. Additionally, fluorescent imaging is used to demonstrate cell selective two‐step gene therapy. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Biophotonicsmentioning

confidence: 99%

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Antkowiak

Torres-Mapa

Gunn‐Moore

et al. 2010

Journal of Biophotonics

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“…Efforts towards miniaturizing electroporation have adopted manipulation of microelectrodes, micropipette and capillaries [15] or microfluidic device-based methods [16,17]. Laser-assisted photoporation has been demonstrated using various laser sources [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional opto-fluidic devices fabricated by ultrashort laser pulses for high throughput single cell detection and processing

et al. 2009

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Three-dimensional flow-through microchannels were fabricated inside bulk fused silica glass via ultrashort pulsed laser direct writing. The device fabrication sequence takes advantage of the nonlinear volumetric absorption in glass and the subsequent preferential chemical etching process. Optical waveguides were also written into the glass specimen and integrated with the fluidic conduits. Flow tests using both fluorescent particles and red blood cells (RBCs) were conducted on various three-dimensional channel configurations. Experiments showed the possibility for laser-induced cell processing inside the microchannels. To evaluate cytometer functionality, RBCs were detected inside the manufactured microchannel via both transmission and fluorescence probing.PACS

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“…Laser-assisted photoporation has been developed for contact-free, non-disruptive, and targeted transfection of individual cells using CW [7] or pulsed [8] lasers. With pulse repetition frequency (PRF) in the kHz range, cavitation bubbles are thermoelastically generated to enable nano-dissection or even membrane perforation.…”

Section: Cavitation and Its Biomedical Implicationsmentioning

confidence: 99%

Probing the bioeffects of cavitation at the single-cell level

Yuan

Sankin

Yang

et al. 2013

The Journal of the Acoustical Society of America

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The primary goal of this dissertation research is to develop an experimental system and associated techniques that can be used to investigate the bioeffects produced by cavitation bubbles at the single cell level. Such information has been lacking due to the randomness and complexity in cavitation inception and subsequent bubble-bubble interaction generated by an acoustic field typically used in therapeutic ultrasound applications.Connection between cavitation activities and bioeffects produced in cells nearby presents another challenge that has not been resolved satisfactorily. In this work, we developed a laser-based system for generating tandem bubbles with a maximum diameter about 50 µm (i.e., on the scale of a single cell) in a microfluidic channel of 25 µm in height and 800 µm in width. We further developed techniques for micropatterning of individual gold dots (15 nm thick and 6 µm in diameter) used for bubble generation, which are precisely aligned at various stand-off distances (SD) from individual islands (32 x32 µm 2 ) coated with fibronectin used for cell adhesion. The dynamics of tandem bubble interaction with resultant jet formation, microstreaming and vortex flow in the microfluidic channel were captured by high-speed imaging and particle image velocimetry (PIV). The deformation of the target cell was recorded by high-speed imaging as well (using a second camera) immediately after the tandem bubble interaction and assessment of membrane strain was aided with 2 µm sized polystyrene beads attached to the cell membrane.

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Photoporation and cell transfection using a violet diode laser

Cited by 78 publications

References 0 publications

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Three-dimensional opto-fluidic devices fabricated by ultrashort laser pulses for high throughput single cell detection and processing

Probing the bioeffects of cavitation at the single-cell level

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