Photoreactivation of Escherichia coli is impaired at high growth temperatures

Xu, Lei; Tian, Changqing; Lü, Xiaohua; Ling, Liefeng; Lv, Jun; M, Wu; Zhu, Guoping

doi:10.1016/j.jphotobiol.2015.03.012

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…Cyanobacterial mutants have been generated via UV‐C for the purpose of genetic analysis of heterocyst‐forming cells, chemical resistance, and antibiotic resistance . A major disadvantage of UV‐C irradiation with cyanobacteria is that it is desirable to have a period of growth in dark conditions immediately after irradiation to prevent photoreactivation, which is the repair of UV‐induced lesions in DNA using light energy . Unfortunately, many cyanobacteria are obligate photoautotrophs, and cannot survive under dark conditions.…”

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

“…128 A major disadvantage of UV-C irradiation with cyanobacteria is that it is desirable to have a period of growth in dark conditions immediately after irradiation to prevent photoreactivation, which is the repair of UV-induced lesions in DNA using light energy. 129 Unfortunately, many cyanobacteria are obligate photoautotrophs, and cannot survive under dark conditions. One way to circumvent this dilemma is to incubate the irradiated culture under yellow light, which allows for growth but prevents photoreactivation.…”

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

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Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Johnson

Gibbons

et al. 2016

Biotechnology Progress

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The rapid increase in worldwide population coupled with the increasing demand for fossil fuels has led to an increased urgency to develop sustainable sources of energy and chemicals from renewable resources. Using microorganisms to produce high-value chemicals and next-generation biofuels is one sustainable option and is the focus of much current research. Cyanobacteria are ideal platform organisms for chemical and biofuel production because they can be genetically engineered to produce a broad range of products directly from CO , H O, and sunlight, and require minimal nutrient inputs. The purpose of this review is to provide an overview on advances that have been or could be made to improve strains of cyanobacteria for industrial purposes. First, the benefits of using cyanobacteria as a platform for chemical and biofuel production are discussed. Next, an overview of cyanobacterial strain improvements by genetic engineering is provided. Finally, mutagenesis techniques to improve the industrial potential of cyanobacteria are described. Along with providing an overview on various areas of research that are currently being investigated to improve the industrial potential of cyanobacteria, this review aims to elucidate potential targets for future research involving cyanobacteria as an industrial microorganism. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1357-1371, 2016.

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Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Johnson

Gibbons

et al. 2016

Biotechnology Progress

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“…Photoreactivation of wild-type R. fascians ATCC 12974, R. fascians oSPL, and R. fascians dSPL was investigated using a protocol described previously ( Xu et al 2015 ) with some modifications. The R. fascians strains were cultured in the cultivation medium (containing 20 g glucose, 1 g yeast extract, 7 g tryptone, 0.38 g K 2 HPO 4 , 0.5 g KH 2 PO 4 and 0.5 g MgSO 4 ·7H 2 O per liter) at 25 °C for ∼3 days to early stationary phase.…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel Class of Photolyases as Possible Ancestors of Their Family

Chen

Wen

et al. 2021

Molecular Biology and Evolution

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UV-irradiation induces the formation of cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts in DNA. These two types of lesions can be directly photorepaired by CPD photolyases and 6-4 photolyases, respectively. Recently, a new class of 6-4 photolyases named iron-Sulphur Bacterial Cryptochromes and Photolyases (FeS-BCPs) were found, which were considered as the ancestors of all photolyases and their homologs — cryptochromes. However, a controversy exists regarding 6-4 photoproducts only constituting ∼10-30% of the total UV-induced lesions that primordial organisms would hardly survive without a CPD repair enzyme. By extensive phylogenetic analyses, we identified a novel class of proteins, all from eubacteria. They have relatively high similarity to class I/III CPD photolyases, especially in the putative substrate-binding and FAD-binding regions. However, these proteins are shorter, and they lack the "N-terminal α/β domain" of normal photolyases. Therefore, we named them Short Photolyase-Like (SPL). Nevertheless, similar to FeS-BCPs, some of SPLs also contain four conserved cysteines, which may also coordinate an iron-sulphur cluster as FeS-BCPs. A member from Rhodococcus fascians was cloned and expressed. It was demonstrated that the protein contains a FAD cofactor and an iron-sulphur cluster, and has CPD repair activity. It was speculated that this novel class of photolyases may be the real ancestors of the cryptochrome/photolyase family.

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“…also has DNA repair enzymes and carotenoids, and their superior activities, may be responsible for the resistance to UV irradiation. In the case of bacteria belonging to the genus Pantoea, only one gene encoding photolyase has been identified in P. agglomerans [110]. It is believed that some of the DNA repair pathways (NER, BER, and MMR) are highly conserved from bacteria to human [98,101,104].…”

Section: Uv Irradiationmentioning

confidence: 99%

Growth Properties and Sensitivities to Various Bactericidal Methods of Cold-Tolerant Microorganisms Isolated from Packed Tofu

et al. 2022

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Disinfection and sterilization against cold-tolerant microorganisms are very important for enhancing food safety and hygiene management under refrigeration conditions. We isolated Exiguobacterium sp. and Pantoea sp. from an immersion solution of packed tofu and evaluated their growth properties and sensitivities to various bactericidal methods. These microorganisms were classified as psychrotrophic bacteria, according to their ability to grow at 4 °C, with an optimum growth temperature of 30 °C. Exiguobacterium sp. and Pantoea sp. were more sensitive to heat and sodium hypochlorite than Escherichia coli (E. coli). Exiguobacterium sp. was sensitive to acetic acid and citric acid, while Pantoea sp. was relatively insensitive and E. coli was insensitive to these organic acids. All microorganisms used in this study were resistant to sodium hydroxide solution (pH 8.00). Exiguobacterium sp. showed resistance to ozonated water and UV; therefore, particular attention should be paid to the contamination of this microorganism in food factories. Both Exiguobacterium sp. and Pantoea sp. were inactivated by pulsed electric field (PEF) treatment that did not cause lethal damage to E. coli. The results obtained in this study suggest that the bactericidal methods targeting the fragile cell membrane with high permeability are effective for the inactivation of cold-tolerant microorganisms.

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Photoreactivation of Escherichia coli is impaired at high growth temperatures

Cited by 14 publications

References 42 publications

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Identification of a Novel Class of Photolyases as Possible Ancestors of Their Family

Growth Properties and Sensitivities to Various Bactericidal Methods of Cold-Tolerant Microorganisms Isolated from Packed Tofu

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