PHOTOSENSITIZATION OF SINGLE‐STRAND BREAKS IN pBR322 DNA BY ROSE BENGAL

Ciulla, Thomas A.; Camp, John R. Van; Rosenfeld, Evan; Kochevar, Irene E.

doi:10.1111/j.1751-1097.1989.tb04109.x

Cited by 73 publications

(41 citation statements)

References 16 publications

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“…Photographs were taken with a digital photocamera Kodak DC256 and the quantification of the spots was achieved by the image analyzer software Quantity One (BIO RAD, Milano Italy). The fraction of supercoiled DNA was calculated as described by Ciulla et al [47] Primer extension and DNA sequencing: Plasmid pUC18 was purified from transformed Escherishia coli strain HB101 by the GeneElute plasmid miniprep kit (Sigma Saint Louis, MI USA) and stored at 4 8C in TE buffer (Tris-HCl (10 mM), EDTA (1 mM); pH 8).…”

Section: Methodsmentioning

confidence: 99%

Indolo[2,3-b]-Quinolizinium Bromide: An Efficient Intercalator with DNA-Photodamaging Properties

et al. 2002

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The associative interactions of indolo[2,3-b]-quinolizinium bromide with DNA and its DNA photocleavage properties were studied in detail. Absorption and emission spectroscopy, linear dichroism, and energy-transfer measurements indicate that the indoloquinolizinium binds to DNA primarily by intercalation, with a preference for GC base pairs. In agreement with this data, the results of primer extension analysis indicate that photocleavage occurrs prevalently at the GC nucleotides. Molecular modeling studies confirm that intercalative stacking between adjacent base pairs is energetically favorable. However, it is also observed that the location of the dye in the minor groove of the DNA is energetically even more favorable. Upon UVA irradiation, the indoloquinolizinium causes single-strand cleavage with an efficiency that varies with the dye-DNA ratio. This observation is rationalized in terms of more efficient photocleavage by the externally bound dye compared with the intercalated one. The kinetics of strand degradation under aerobic and anaerobic conditions suggest that a Type I reaction occurs, that is, radical-mediated DNA damage.

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Section: Methodsmentioning

confidence: 99%

Indolo[2,3-b]-Quinolizinium Bromide: An Efficient Intercalator with DNA-Photodamaging Properties

et al. 2002

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“…The fractions of supercoiled DNA (Form I) and open circular (Form II) were calculated as described. 25 (1)…”

Section: Methodsmentioning

confidence: 99%

DNA-binding and DNA-photocleavaging properties of 12a,14a-diazoniapentaphene

Viola¹,

Ihmels²,

Kraußer³

et al. 2004

Arkivoc

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“…Photographs were taken with a digital photocamera Kodak DC256 and the quantitation of the bands was achieved by image analyzer software Quantity One (BioRad, I-Milano). The fractions of supercoiled DNA (Form I) and open circular (Form II) were calculated as described in [46].…”

mentioning

confidence: 99%

Photophysical and Phototoxic Properties of the Antibacterial Fluoroquinolones Levofloxacin and Moxifloxacin

Viola

Facciolo

Canton

et al. 2004

Chemistry & Biodiversity

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Two antibacterial fluoroquinolones, levofloxacin and moxifloxacin, were investigated to evaluate their photophysical properties and to explore the mechanism of their phototoxicity. Photophysical experiments were carried out in aqueous solution by stationary and time-resolved fluorimetry, and by laser flash photolysis, to obtain information on the various decay pathways of the excited states of the drugs and on transient species formed upon irradiation. The results obtained show that levofloxacin is able to photosensitize red blood cell lysis in an oxygen-independent way and induce a high decrease in cell viability after UVA irradiation, although to a lesser degree than the racemic mixture ofloxacin. Moxifloxacin, which is an 8-MeO-substituted fluoroquinolone, is less phototoxic than the other compounds. Cellular phototoxicity was inhibited by the addition of superoxide dismutase, catalase, and free radical and hydroxyl radical scavengers (BHA, GSH, mannitol, and DMTU), indicating the involvement of superoxide anion and/or a radical mechanism in their cytotoxicity. A good correlation was observed between lipid peroxidation, protein photodamage, and cellular phototoxicity, indicating that test compounds exert their toxic effects mainly in the cellular membrane. Experiments carried out on pBR322 DNA show that these derivatives do not significantly photocleave DNA directly, but single-strand breaks were evidenced after treatment of photosensitized DNA by two base-excision-repair enzymes, and Endo III.

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PHOTOSENSITIZATION OF SINGLE‐STRAND BREAKS IN pBR322 DNA BY ROSE BENGAL

Cited by 73 publications

References 16 publications

Indolo[2,3-b]-Quinolizinium Bromide: An Efficient Intercalator with DNA-Photodamaging Properties

Indolo[2,3-b]-Quinolizinium Bromide: An Efficient Intercalator with DNA-Photodamaging Properties

DNA-binding and DNA-photocleavaging properties of 12a,14a-diazoniapentaphene

Photophysical and Phototoxic Properties of the Antibacterial Fluoroquinolones Levofloxacin and Moxifloxacin

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