Rose bengal photosensitized the formation of frank single-strand breaks (SSBs) in double-stranded, supercoiled pBR322 DNA as measured by neutral agarose electrophoresis. The yield of SSBs followed first order kinetics with respect to light fluence and dye concentration. The efficiency of cleavage was more than 20 times greater in an argon atmosphere than in an oxygen atmosphere. The quantum yield in an air atmosphere was 1.7 (+/- 0.3) X 10(-8). Sodium azide quenched the cleavage more efficiently in an oxygen atmosphere than when the oxygen concentration was reduced. Isopropanol and mannitol were poor quenchers; ribose-5-phosphate and guanosine-5'-monophosphate did not quench the cleavage. Substituting D2O for H2O increased the yield of SSBs in both oxygen and oxygen-depleted atmospheres. The results are consistent with initiation of cleavage by reaction of the triplet state of rose bengal (or a radical derived from it) with DNA. In the presence of oxygen, an additional mechanism is introduced.

We apply visualization techniques to user profiles and repository metadata from the GitHub source code hosting service. Our motivation is to identify patterns within this development community that might otherwise remain obscured. Such patterns include the effect of geographic distance on developer relationships, social connectivity and influence among cities, and variation in projectspecific contribution styles (e.g., centralized vs. distributed). Our analysis examines directed graphs in which nodes represent users' geographic locations and edges represent (a) follower relationships, (b) successive commits, or (c) contributions to the same project. We inspect this data using a set of visualization techniques: geo-scatter maps, small multiple displays, and matrix diagrams. Using these representations, and tools based on them, we develop hypotheses about the larger GitHub community that would be difficult to discern using traditional lists, tables, or descriptive statistics. These methods are not intended to provide conclusive answers; instead, they provide a way for researchers to explore the question space and communicate initial insights.

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